In vivo and in vitro elongation factor-RNA interaction data provide a missing link in understanding how processive elongation complexes are formed on active genes and disassembled at the end of genes.

The elongation rate of RNA Polymerase II varies greatly between and along genes, as this enzyme accelerates from stable pausing to rapid elongation within genes, and is influenced by CG-content, exons and chromatin.

CDK9 inhibition in human cells uncovers that Pol II pause duration regulates the frequency of productive transcription initiation.

Pol II-mediated transcription and chromatin looping of CDKN1A are repressed by MAF1 through the regulation of Pol III activity.

JMJD6 helps BRD4 to recruit CDK9 to RNA Polymerase II by disrupting 7SK snRNP complex.

Cleavage/polyadenylation, the process generating mRNA 3' ends, is linked at the nucleotide level to the position of RNA polymerase II, indicating a spatial coupling so polyadenylation occurs rapidly upon emergence of the nascent RNA from the Pol II elongation complex.

NET-seq analysis of 41 S. cerevisiae transcription regulatory factors uncovers their differential and opposing effects on Pol II transcription elongation and antisense transcription, revealing that wild-type transcription is balanced by the joint impact of many regulators.

TATA-box binding protein is not required for RNA Polymerase II transcription in mouse embryonic stem cells.

Arabidopsis RNA processing factor SERRATE associates with the chromatin of intronless genes, which are usually expressed at low levels, to enhance polymerase II association.

Ssl2 processivity determines the window for transcription start site selection during promoter scanning by RNA polymerase II in Saccharomyces cerevisiae.