A new high-throughput method for single-cell RNA-seq in yeast cells shows how stochastic expression of glucose-repressed genes contributes to cell-to-cell differences during adaptation to an environmental change.
Gene expression was analyzed in hippocampal principal cells, enabling cellular phenotyping and revealing novel organizational principles; complementing this, a publicly available website is released to provide outside analysis and visualization of hippocampal RNA-seq data in an accessible fashion.
RNA binding protein FOX-1 functions as a dose-dependent X-signal element to communicate X-chromosome number and determine nematode sex by controlling alternative non-productive pre-mRNA splicing of the master sex-determination switch gene.
A time-course of single nuclei RNA-seq of the mouse placenta identifies trophoblast subtypes and the genes, signaling events, and transcriptional networks important for their differentiation, maintenance, and function.
Tn5 transposase has direct tagmentation activity towards RNA/DNA hybrids, which is harnessed as a more convenient and faster RNA-seq library construction method and will benefit RNA and chromatin research.
RNA sequencing of individuals within a wild baboon population reveals extensive power to detect functional regulatory variation, and suggests that the set of genes affected by such variation may be conserved across species.