M1 alanyl aminopeptidase is chemically validated as an attractive antimalarial target by confirming the on-target activity of a novel selective inhibitor using chemoproteomics and metabolomics-based drug target deconvolution approaches.

Hypothemycin, which inhibits a number of protein kinases, kills the T. brucei parasites that cause sleeping sickness and reveals new therapeutic targets for the disorder.

Deciphering the mechanism of action of a large family of natural and synthetic cytotoxic lipids bioactivated by enantiospecific oxidation into protein-reactive species inspires the development of enzyme-specific prodrugs.

Myristoylation of a secreted protein, identified in a global myristoylation analysis of the eukaryotic parasite Toxoplasma gondii, is important for host cell invasion.

Cell-specific pathways regulate lipid transmitters and neuroinflammation in the brain.

An approachable framework for the scalable implementation of proteome-wide thermal shift assays to assess drug mechanisms of action.

Phosphatidylinositol 3-phosphate interacts with PfHsp70-1 and stabilizes the Plasmodium digestive vacuole under febrile temperatures.

Palmitate turnover is controlled by distinct enzyme families with different specificities.