In a synthetic system, spreading of chromatin-mediated silencing to nearby genes depends on distance and can bypass genetic insulators, while reactivation after release of gene targeting is coordinated by insulators and promoters.

Certain types of 3D chromatin loops are easy to predict from existing or easily obtainable 2D information, which benefits gene expression studies in tissues/cells/organisms without extensive pre-existing 3D information.

Fruit fly chromosomes are divided into discrete structural domains by regions of decompacted chromatin, suggesting a novel model for the formation of a known class of genetic elements.

A novel synthetic DNA cassette of CTCF-binding sites combined with the drug-controllable induction system of heterochromatin enabled switchable blocking of chromatin conformation and gene-enhancer interaction.

The patterns of chromatin architecture that underlie the initial embryonic cell fate decisions are established during a period of intense cell cycle activity, and these patterns are stably maintained even in highly condensed mitotic chromatin.

A chromatin boundary factor that is only active during early embryo development has been discovered in Drosophila.

Biochemical and biological characterization of insulator complex SUMM4 reveals that, in addition to disrupting enhancer–promoter interactions and establishing chromatin barriers, it functions to delay DNA replication, thus implicating architectural proteins in shaping spatiotemporal patterns of replication.

Boundary:boundary pairing generates either stem-loop or circle-loop TADs, and these loop topologies differ in how they impact chromosome structure and genetic activities.

A novel mechanism of gene regulation is discovered wherein a specific long non-coding RNA abolishes the blocking activity of chromatin insulator in the Drosophila HOX gene cluster.

How gene expression noise is regulated is critical for cell fate and tissue patterning.