Solvent shift assays such as solvent proteome profiling and solvent-PISA are valuable tools for identifying protein-ligand interactions on a proteome-wide scale and can be used to determine drug target engagement, which should benefit future drug discovery efforts.

A drug-like molecule called ISRIB, which activates the translation initiation factor eIF2B, antagonizes stress responses as diverse as protein misfolding and nutrient deprivation, and restores protein synthesis, enhancing memory.

Kinase conformation reporters reveal how protein kinase activity conformations are influenced by regulatory protein interactions, patient mutations, and cellular drug binding, aiding in systematically anticipating treatment efficacy and designing patient-tailored therapies.

Primary auditory cortex shows population-level correlates of sound categorization, with categorical patterns emerging during sound presentation and task engagement leading to asymmetrical representation of stimuli, indicating an early involvement in stimulus categorization.

β-Catenin-mediated expansion of nephron progenitors is independent of direct β-catenin/chromatin engagement, while progenitor induction proceeds with a β-catenin-driven switch of repressive TCFL1/TCFL2 to activating TCF7/LEF1 factors on transcriptionally poised enhancers.

Modeling the dynamics of immunological synapses informs pharmacodynamics and treatment resistance to bispecific T cell engagers.

The dimer destabilizers cause a dimer-to-monomer equilibrium shift favoring the human thymidylate synthase monomer more degradable by the proteasome, thus breaking the long-standing link between inhibition and enhanced expression of the protein to fight cancer drug resistance.

An approachable framework for the scalable implementation of proteome-wide thermal shift assays to assess drug mechanisms of action.

Hypothemycin, which inhibits a number of protein kinases, kills the T. brucei parasites that cause sleeping sickness and reveals new therapeutic targets for the disorder.

A direct relationship between pupil diameter and electrophysiological correlates of attention, sensory stimulus processing and target detection was observed demonstrating that arousal has a substantial influence on perceptual decision-making.