In vitro and in cellulo characterization of oligomerization by the cytoplasmic Wnt effector dishevelled and its partner Axin provide new mechanistic principles for Wnt/β-catenin signaling.

Genome editing, advanced live-cell microscopy, and computational modeling were combined to measure WNT/CTNNB1 signaling parameters at the single molecule level, revealing critical regulatory nodes in this important signal transduction pathway.

Kif26b, a new component of the evolutionarily conserved Wnt5a-Ror signaling pathway, is controlled by regulated proteolysis and plays crucial roles in mediating vertebrate embryonic tissue morphogenesis.

Selective activation of FZD7 signaling with an engineered WNT mimetic promotes early developmental programs, including endodermal lineage specification, in human pluripotent stem cells.

Imaging of single cells within a cell population shows the correlation between subcellular accumulation of β-catenin and the transcriptional activation of a target gene.

Spatially distinct ectodermal Wnt and endodermal Shh signals serve to polarize the skate gill skeleton.

β-catenin, a key effector of the Wnt signaling pathway, is transported into the nucleus via a direct interaction between its PY-NLS and TNPO1 offering new potential targets for cancer therapeutics.

Cryo-EM structure of monomeric human Frizzled5 was determined with a universal fiducial antibody at 3.7 Å overall resolution, which supports a simple Fzd/LRP6 heterodimerization mechanism of canonical Wnt/β-catenin signaling.

Insights into the roles and mechanisms of two of the miRNAs upregulated in human breast cancer stem cells in at least some breast cancers are presented.

Analysis of axial polarity distributions shows that Wnt5a regulates collective cell migration in vivo by stabilizing vinculin at adherens junctions and fine-tuning mechanocoupling between neighbouring cells.