A high-throughput comparison of substrate specificities of the Src-family kinases Lck and c-Src against a library of proteome-derived phosphorylation sites reveals that Lck has evolved divergent electrostatic features reflecting its involvement in T-cell signaling.
Doubletime kinase stabilizes the PER/TIM complex and regulates its transcriptional inhibition function to delay circadian transcriptional activity, helping sustain 24-hour periodicity in the circadian clock.
A gene network analysis approach reveals a conserved small regulatory RNA that is crucial for bacterial cell survival across distinct stress conditions.
Ninning Liu, Gheorghe Chistol ... Carlos Bustamante
Probing the DNA motor SpoIIIE at the single-molecule level has revealed its force-generating step, rich translocation dynamics during motor operation and a novel, bi-phasic mechanical response to opposing force.
A new biochemical method tests whether or not the pre-existing RNA structural correlations couple small molecule binding to gene expression in a paradigmatic riboswitch.
Jieqiong Gao, Lars Langemeyer ... Christian Ungermann
The endosomal GEF Mon1-Ccz1 binds to Atg8 on autophagosomes, and recruits its substrate, the Rab7-like Ypt7, which then mediates fusion of the autophagosomes with the lysosome.
ADGRG2, an orphan GPCR, when coupled to CFTR via a regional Gq signaling on the apical membrane, acts to regulate efferent duct fluid reabsorption making it essential for male fertility.
Adriana Degrossoli, Alexandra Müller ... Lars I Leichert
The use of genetically encoded redox sensors in phagocytized bacteria reveals that, among the toxic cocktail of oxidants released into the neutrophil's phagolysosome, HOCl is the main component responsible for the oxidative modification of bacterial protein thiols.
Anassuya Ramachandran, Pedro Vizán ... Caroline S Hill
SMAD1/5 signaling is essential for the full transforming growth factor β (TGF-β)-induced transcriptional program and physiological responses and is induced via a novel receptor activation mechanism, involving two distinct type I receptors.
