Alejandro J Brenes, Eva Griesser ... Angus I Lamond
Human induced pluripotent stem cells and embryonic stem cells express a similar set of proteins, but the levels of cytoplasmic and mitochondrial proteins vary, with concomitant effects on phenotypes including transport and mitochondrial function.
A novel pair of isobaric crosslinking reagents is described, which allow relative quantification of crosslinker-modified peptides during mass spectrometry analysis for comparative structural studies of proteins and protein complexes.
SLC35G1 is the first highly chloride-sensitive transporter localized on the basolateral membrane of intestinal epithelial cells, responsible for citrate absorption.
Robust NMR approach allowed simultaneous detection of substrate cleavage site and real-time kinetics of hydrolysis for Staphylococcus aureus peptidoglycan hydrolases lysostaphin and LytM, and revealed D-alanyl-glycine activity for LytM.
For mRNA decapping by enhancing DCP2's mRNA-binding affinity and regulating distinct biological processes through DCP1a and DCP1b, human DCP1 is essential.