Weiwei Wang, Kouki K Touhara ... Roderick MacKinnon
Determining how GIRK2 activity depends on the concentration of Gβγ subunits in lipid membranes at different Na+ concentrations has allowed the Gβγ concentration generated during GABAB activation in neurons to be estimated.
The GIRK1 subunit contains a defective Na+-binding site but behaves as if it is permanently bound to a sodium ion, and therefore increases the affinity of Gβγ to GIRK1/4 hetero-tetrameric channels in lipid membranes.
Shaogeng Tang, Nicholas J Buchkovich ... Scott D Emr
Building on previous work (Tang et al., 2015), novel ESCRT-III subunit Snf7 auto-activation mutants are used to reveal two parallel ubiquitin-dependent pathways during multivesicular endosome biogenesis.
Moitrayee Bhattacharyya, Margaret M Stratton ... John Kuriyan
Closed-ring and open-spiral assembly of the multi-subunit neuronal kinase CaMKII suggest a mechanism for how active subunits shuttle between individual assemblies, propagating stimulatory signals.
Mary K Thompson, Maria F Rojas-Duran ... Wendy V Gilbert
Asc1/RACK1 promotes the translation of mRNAs associated with the translational closed loop complex, which have short open reading frames and encode proteins required for core metabolic processes.
Unlike other similar enzymes, the antimicrobial enzyme PlyC can interact with and translocate eukaryotic membranes, and then lyse and kill intracellular bacteria.
Regulatory elements in an mRNA that act in cis to influence translation can also exert their effects in trans, in a process dependent on assembly of the mRNAs into particles.
