Parkin ubiquitination of damaged mitochondria recruits the Rab GEF, RABGEF1, to regulate downstream Rab cycles and ATG9A recruitment to growing phagophores that are participating in mitophagy.
A new approach using combination of electron microscopy (EM) and high-speed atomic force microscopy (HS-AFM) clearly demonstrates dynamics of dynamin-amphiphysin complexes during membrane constriction and fission suggesting a novel 'clusterase' model of the dynamin-mediated membrane fission.
To promote longevity under heat stress shares yeast aging factors with progeny through a down-regulation of the diffusion barrier in the membranes between the mitotic cells.
Patrick A Murphy, Vincent L Butty ... Richard O Hynes
Transcriptome analysis reveals an alternative splicing program induced in the arterial endothelium under low-flow inflammatory conditions by platelet and macrophage recruitment and dependent upon the RNA-binding splice factor Rbfox2.
Hippocampal neurons undergo bi-stable behavior as neurites emerge, and the dominance of either state is determined by environmental mechanical properties and paxillin-associated cellular endocytic activities.
B Duygu Özpolat, Mette Handberg-Thorsager ... Guillaume Balavoine
Lineage tracing at single-cell resolution reveals the presence of mesoteloblasts, the embryonic origin of mesodermal growth zone cells, and diverse cell cycling patterns of these lineages in the 'Polychaete' annelid Platynereis.
Scott A Kanner, Travis Morgenstern, Henry M Colecraft
Engineered E3 ubiquitin ligases are utilized to elucidate mechanisms underlying ubiquitin regulation of membrane proteins, and to achieve robust post-translational functional knockdown of ion channels.
An optimized 3D fluorescence co-localization method is a useful toolkit to obtain cellular 3D separations between green and red labeled protein domains with nanometer-scale accuracy using light microscopy.
Aviv Regev, Sarah A Teichmann ... Human Cell Atlas Meeting Participants
Advances in techniques for analysing single cells and tissues have inspired an international effort to create comprehensive reference maps of all human cells - the fundamental units of life - as a basis for both understanding human health and diagnosing, monitoring and treating disease.
