Somsakul Pop Wongpalee, Ajay Vashisht ... Douglas L Black
A protein regulator of alternative pre-mRNA splicing arrests spliceosome assembly at a previously unrecognized step that defines new intermediate stages in the formation of exon complexes.
Sarah J Hainer, Kurtis N McCannell ... Thomas G Fazzio
Two methyl-binding domain proteins have interdependent functions in embryonic stem cells, and this interdependency extends to the DNA methylation/hydroxymethylation machinery.
Rachael Di Santo, Soufiane Aboulhouda, David E Weinberg
The unspliced HAC1 mRNA does not give rise to detectable protein in budding yeast, despite its cytoplasmic localization, due to a two-part post-transcriptional silencing mechanism.
Cooperative association of a histone-binding complex with pairs of appropriately modified nucleosomes, which form fundamental units of binding, mediates selective heterochromatin assembly and spreading.
Max A Horlbeck, Luke A Gilbert ... Jonathan S Weissman
Integrated modeling of sgRNA positioning, chromatin accessibility, and sequence features enables accurate prediction of effective target sites for CRISPR-mediated transcriptional modulation and design of highly active libraries for genome-scale genetic screens.
Gudrun Böhmdorfer, Shriya Sethuraman ... Andrzej T Wierzbicki
Genome-wide identification of long non-coding RNAs produced by RNA Polymerase V identifies their role in heterochromatin maintenance on the boundaries of repressed regions.
The E3 ubiquitin ligase activity of polycomb repressive complex 1 is stimulated by RYBP to support a histone modification-dependent communication between polycomb repressive complexes in mice.
