New methods reveal that complex local splicing variations are more prevalent in animals than previously appreciated, and demonstrate that local splicing variations are relevant for studies of development, gene regulation and neurodegenerative diseases.
Loss-of-function screening identified transglutaminase 2 (TGM2) as a putative tumor suppressor in the TP53 pathway and revealed that TGM2-mediated autophagy and CDKN1A-mediated cell cycle arrest are two critical barriers that prevent oncogenic transformation.
Candice L Wike, Hillary K Graves ... Jessica K Tyler
A histone modification that alters the nucleosome structure occurs in mitosis and promotes chromosome packaging and the timely removal of condensin I and cohesion, to achieve chromosome segregation.
By properly accounting for gene copy number and cell-cycle effects, single cell snapshots of nascent and mature mRNA can be used to unveil the stochastic kinetics of gene activity.
Barbora Malecova, Alessandra Dall'Agnese ... Pier Lorenzo Puri
Genetic and biochemical evidence shows that the basal transcription machinery of muscle cells invariably relies on TBP/TFIID because TBP2 is not expressed in muscle cells, and thus resolves a longstanding issue raised by previous conflicting data.
Andrew R Conery, Richard C Centore ... Robert J Sims III
CBP/EP300 bromodomain inhibitors have a therapeutic application in oncology via targeting the IRF4 transcriptional program, a clinically validated network critical for multiple myeloma cell viability.
