An evolution-guided framework is proposed to accelerate the discovery and therapeutic targeting of understudied dark channels by integrating sequence, structure, and functional data from diverse organisms.
A fully computationally designed SaCas9 variant expands PAM recognition to NNNRRT, achieving up to 116-fold higher editing at noncanonical sites while matching the performance of experimentally evolved variants.
The first all-atom models of the mycobacterial outer membrane reveal how lipid organization and asymmetry generate a structurally heterogeneous barrier that underlies its unique permeability properties.
Real-time nanopore transcriptomics enables early detection of expression changes and sample quality, offering a rapid, cost-effective strategy for experimental quality control and transcriptomic analysis.
