A combination of genetic and biochemical approaches suggest that yeast Rev7 promotes DNA double-strand break repair via NHEJ and inhibits homologous recombination by blocking Mre11 nuclease and Rad50’s ATPase activities.
Thomas E Forman, Marcin P Sajek ... Katherine A Fantauzzo
Upon PDGFRα signaling in mouse embryonic palatal mesenchyme cells, Srsf3 exhibits differential transcript binding, resulting in retention of the receptor in early endosomes and increased downstream PI3K-mediated Akt signaling.
Nasa Sinnott-Armstrong, Stanley Fields ... Christine Queitsch
Multiplexed assays of variant effect are an increasingly important tool in genetic research and including more context specificity in their design would aid interpretation and discovery.
Stephanie Guillet, Tomi Lazarov ... Frédéric Geissmann
Loss-of-function variants of human ACK1 and BRK kinase underlie systemic lupus erythematosus in young patients from multiplex families and disrupt the anti-inflammatory response of macrophages to apoptotic cells.
A combined approach of genome sequencing and fluctuation assays shows that antibiotic pressure does not induce adaptive mutations in Mycobacterium smegmatis, supporting drug resistance as a consequence of phenotypic tolerance.
