A versatile invertible gene trap vector is generated and inserted into a defined genomic locus in zebrafish via homologous recombination, demonstrating an efficient genetic approach to performing conditional knockout analysis.
Kerstin Seidel, Pauline Marangoni ... Ophir D Klein
Gene co-expression analysis identifies coherent transcriptional patterns driven by distinct cell types in the mouse incisor, and functional studies of candidate genes reveal how the tissues are maintained through stem cell-fueled renewal.
Paula Freire-Pritchett, Stefan Schoenfelder ... Mikhail Spivakov
Promoter interactome maps in human embryonic stem cells (ESCs) and ESC-derived early neuroectodermal progenitors link distal enhancers to putative target genes, reveal lineage-specific cis-regulatory architecture and shed light on the logic of gene regulation by multiple enhancers.
The chromatin remodeller BRG1 is recruited to pluripotency-associated gene regulatory elements by the pioneer transcription factor OCT4 to support further transcription factor binding and gene regulation.
Genetic and biochemical analyses reveal that PERK is important for the survival of activated satellite stem cells during regeneration of injured skeletal muscle.
Mitsuhiro Endoh, Takaho A Endo ... Haruhiko Koseki
PCGF6 links sequence specific target recognition by the MAX/MGA transcription factor complex to PRC1 (polycomb repressive complex 1) -dependent transcriptional silencing of germ cell-specific genes in mouse pluripotent stem cells.
Tomoya Hasegawa, Christopher J Hall ... Atsushi Kawakami
The Interleukin 1 beta signaling and tissue inflammation act as a double-edged sword: they are required for regeneration, but when in excess, they impair tissue regeneration and induce apoptosis.
Mammalian germ layer differentiation is characterized by transitions through discrete cell states, which are transcriptionally as well as functionally distinct.
Myomaker is activated on muscle stem cells to promote their fusion with myofibers, which is essential for induction of pro-growth signaling pathways and physiological muscle hypertrophy.
