1. Steven J Sandler  Is a corresponding author
  1. University of Massachusetts, United States

We all have DNA, and we need to replicate this DNA in order to grow and reproduce. Critically, however, we also need to protect our DNA from damage, or to repair it when it gets damaged. Sources of damage include certain chemicals produced during metabolism and various forms of radiation. Some DNA repair pathways are relatively simple: an enzyme called photolyase, for example, can repair the damage caused by exposure to ultraviolet radiation from the sun in a single reaction. However, it takes many different proteins to repair the train wreck that is caused when a DNA replication fork runs into a section of DNA damage during the process of DNA replication.

Given the importance of DNA, it is no wonder that the pathways for DNA repair have been largely conserved throughout all three kingdoms of life. That being said, are we all optimized for DNA repair? Could we do it better? This is an important consideration because we live in an environment that can damage DNA in many different ways and, if this damage is not repaired, it can sometimes lead to diseases such as cancer. Now, in eLife, Michael Cox of the University of Wisconsin-Madison and co-workers—including Rose Byrne and Audrey Klingele as joint first authors—report that mutations in just three genes can allow the bacteria Escherichia coli to improve its ability to repair DNA by a factor of 1000. Might we do the same?

The ability to repair DNA is not the same for all organisms. The bacteria Deinococcus radiodurans, for example, is several orders of magnitude more resistant to ionizing radiation than E. coli. Why? Does D. rad have more or different DNA repair pathways? The D. rad genome contains approximately the same repertoire of repair enzymes as E. coli. Does this mean that D. rad has a higher concentration of repair enzymes? Or perhaps the repair enzymes in D. rad are just better at their doing their jobs because they have higher specific activities and/or binding affinities? It is well known that cells respond to DNA damage by expressing DNA repair proteins. Perhaps D. rad responds faster than E. coli, or perhaps its response has a greater amplitude? Maybe the increased resistance is due to something else, such as the way that DNA is packaged inside the nucleus. Or perhaps D. rad is better able to detoxify the reactive oxygen species created by the radiation and/or to cope with damaged proteins?

In 2009, Cox and co-workers posed a different question: is it possible to make E. coli more resistant to ionizing radiation? Given the complexity of DNA repair pathways and gene regulation, this was akin to trying to untie a Gordian Knot. To tackle this problem, they used the combined power of in vitro evolution and bacteria genetics and let the bacteria untie the knot for them. They exposed cultures of E. coli to devastating amounts of ionizing radiation, isolated those that managed to survive, and repeated this process. After 20 cycles of this process, the cultures of E. coli were 3–4 orders of magnitude more resistant to ionizing radiation than the starting E. coli strains (Harris et al., 2009).

DNA sequence analysis revealed that each evolved strain had between 40–80 mutations. Moreover, many of these mutations mapped onto genes that were known to be associated with DNA repair, recombination, DNA replication, proteolysis and cell division, although some of the mutations mapped onto genes of unknown function. But which were the important genes and mutations?

Now Cox and co-workers—who are based at the University of Wisconsin, the DOE Joint Genome Institute and Louisiana State University—have answered this question by continuing the E. coli evolution experiments and characterizing the DNA sequences of the strains that show high levels of resistance to ionizing radiation (Byrne et al., 2014).

They developed criteria to discern the important mutations from the 40–80 mutations in each strain. This narrowed their focus to single mutations in eight genes: recA, dnaB and yfjK (which are involved in DNA metabolism), rsxB and gsiB (suppression of oxidative damage), wcaK and nanE (cell wall biosynthesis), and glpD (basic respiration). By transferring these mutations, both singly and in combinations, to fresh strains of E. coli, and by removing them from evolved strains of the bacteria, they were able to determine that mutations to three genes—recA, dnaB and yfjK—accounted for about 1000-fold of the increased resistance to ionizing radiation. Moreover, recA and dnaB were in the same DNA repair pathways, while yfjK was in another repair pathway. The other mutations made measureable, but smaller contributions. Cox and co-workers also checked for global changes in the transcriptome, the metablome and metal ion content and found none, so the mutations in these three genes must be the primary reason for the increase in resistance.

The important and overwhelming conclusion from this study is that organisms can increase their resistance to ionizing radiation by 3–4 orders of magnitude and that the increase can be traced back to relatively few mutations. Once the reasons for this increase have been understood in greater detail, these results will have implications for our knowledge of DNA repair in a wide range of species, including humans.


Article and author information

Author details

  1. Steven J Sandler

    Department of Microbiology, University of Massachusetts, Amherst, United States
    For correspondence
    Competing interests
    The author declares that no competing interests exist.

Publication history

  1. Version of Record published: March 4, 2014 (version 1)


© 2014, Sandler

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.


  • 2,017
    Page views
  • 27
  • 0

Article citation count generated by polling the highest count across the following sources: Crossref, PubMed Central, Scopus.

Download links

A two-part list of links to download the article, or parts of the article, in various formats.

Downloads (link to download the article as PDF)

Open citations (links to open the citations from this article in various online reference manager services)

Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)

  1. Steven J Sandler
DNA Repair: Vive la résistance!
eLife 3:e02387.

Further reading

  1. Single mutations in three genes can dramatically increase the ability of E. coli to survive ionizing radiation.

    1. Cell Biology
    2. Chromosomes and Gene Expression
    Brandt Warecki, Simon William Abraham Titen ... William Sullivan
    Research Article

    Wolbachia, a vertically transmitted endosymbiont infecting many insects, spreads rapidly through uninfected populations by a mechanism known as cytoplasmic incompatibility (CI). In CI, a paternally delivered modification of the sperm leads to chromatin defects and lethality during and after the first mitosis of embryonic development in multiple species. However, whether CI-induced defects in later stage embryos are a consequence of the first division errors or caused by independent defects remains unresolved. To address this question, we focused on ~1/3 of embryos from CI crosses in Drosophila simulans that develop apparently normally through the first and subsequent pre-blastoderm divisions before exhibiting mitotic errors during the mid-blastula transition and gastrulation. We performed single embryo PCR and whole genome sequencing to find a large percentage of these developed CI-derived embryos bypass the first division defect. Using fluorescence in situ hybridization, we find increased chromosome segregation errors in gastrulating CI-derived embryos that had avoided the first division defect. Thus, Wolbachia action in the sperm induces developmentally deferred defects that are not a consequence of the first division errors. Like the immediate defect, the delayed defect is rescued through crosses to infected females. These studies inform current models on the molecular and cellular basis of CI.