The re-assembly of chromatin following DNA replication is a critical event in the maintenance of genome integrity. Histone H3 acetylation at K56 and phosphorylation at T45 are two important chromatin modifications that accompany chromatin assembly. Here we have identified the protein kinase Pkc1 as a key regulator that coordinates the deposition of these modifications in S. cerevisiae under conditions of replicative stress. Pkc1 phosphorylates the histone acetyl transferase Rtt109 and promotes its ability to acetylate H3K56. Our data also reveal novel cross-talk between two different histone modifications as Pkc1 also enhances H3T45 phosphorylation and this modification is required for H3K56 acetylation. Our data therefore uncover an important role for Pkc1 in coordinating the deposition of two different histone modifications that are important for chromatin assembly.https://doi.org/10.7554/eLife.09886.001
Prior to cell division, DNA must be copied so that each new cell gets a complete copy of the cell’s genetic instructions. But DNA is so long that it is stored in a heavily compacted form in the nucleus of the cell, with the strands of DNA coiled around several proteins called histones. Before the DNA is copied, it must be unfurled. Then each new copy of DNA must be repackaged to fit compactly inside the nucleus of each new cell.
If errors occur in the process of copying DNA, it can lead to genetic mutations that may cause diseases like cancer. To prevent this, cells have mechanisms to identify errors and correct them before the DNA is repackaged. This requires a pause to allow the repairs to occur before the DNA recoils. However, it is not completely clear how this process is controlled.
Now, Darieva et al. show that an enzyme called protein kinase C (or Pkc1 for short) is essential to repackaging DNA after the errors are corrected. Several experiments showed that Pkc1 plays an important role when cells were exposed to stressful conditions that potentially cause errors in DNA copying. Specifically, Pkc1 helps prepare the third histone protein (histone H3) so that DNA can recoil around it. Pkc1 waits until the stressful conditions have passed and the DNA has been repaired to make the necessary changes.
Once the stress has passed, Pkc1 adds a phosphate to another enzyme called Rtt109 that prepares the histone. The Pkc1 simultaneously contributes to another necessary change to histone H3. These new details about DNA repackaging may help researchers understand how cells protect against DNA copying errors, and how this process goes wrong in cancer.https://doi.org/10.7554/eLife.09886.002
During the cell cycle one of the key processes following DNA replication is the re-assembly of chromatin on the newly replicated DNA. This involves the re-deposition of both pre-existing and newly synthesised histones into intact nucleosomes (reviewed in Groth et al., 2007; Ransom et al., 2010; Gurard-Levin et al., 2014). This process must be tightly controlled and in S. cerevisiae one key event is the Rtt109-mediated modification of histone H3 by acetylation of lysine 56 (H3K56) (Han et al., 2007; Driscoll et al., 2007). Rtt109 cooperates with two different histone chaperones Asf1 and Vps75 (Tsubota et al., 2007; Driscoll et al., 2007) and although it is generally accepted that Asf1 is the major chaperone involved in promoting H3K56 acetylation (Keck et al., 2011; Tang et al., 2011), recent evidence suggests a role for Vps75 in this process (Radovani et al., 2013). However, the signalling pathways controlling Rtt109-mediated H3K56 acetylation are unknown. Another important modification that occurs coincidentally with DNA replication is H3 phosphorylation at threonine T45 (H3T45) but current evidence suggests that this modification has no relationship with the closely located H3K56 acetylation event (Baker et al., 2010). In the context of an unperturbed cell cycle, Cdc7-Dbf4 has been shown to be a major kinase involved in controlling H3T45 phosphorylation levels (Baker et al., 2010).
S. cerevisiae possesses one protein kinase C isoform, Pkc1, and it is known to play a major role in cell wall integrity signalling (reviewed in Levin, 2005). Pkc1 has a growing number of nuclear functions and through genetic experiments this protein kinase has previously been implicated in cell cycle events (reviewed in Levin, 2005). Part of this role can be attributed to its connection to pathways sensing cell wall integrity and its effects on the activity of the downstream MAP kinase pathway. However MAP kinase-independent roles for Pkc1 have emerged and Pkc1 was recently shown to directly affect the cell cycle by inhibiting the activity of the transcriptional coactivator protein Ndd1 (Darieva et al., 2012). This finding suggested that Pkc1p might act as a checkpoint kinase under conditions of replicative stress where progression to G2 phase is inhibited. However, mutant strains containing ndd1 mutant alleles lacking the Pkc1 phosphorylation sites did not fully re-capitulate the pkc1 mutant phenotypes, indicating that additional defects are associated with loss of Pkc1 activity. Indeed, pkc1 mutants are sensitive to hydroxurea (HU) treatment (Queralt and Igual, 2005), suggesting a potential role for Pkc1 in controlling the response to replicative stress. It is unclear how Pkc1 might affect replicative stress but previous genetic studies have linked PKC1 and components of the RSC chromatin remodelling complex and Pkc1 overexpression can suppress the defects associated with RSC mutants (Chai et al., 2005; Hosotani et al., 2001; reviewed in Levin et al., 2005). This is suggestive of a potential role for Pkc1 in regulating chromatin assembly during DNA replication.
Here, we have investigated the potential role of Pkc1 in controlling chromatin modifications during replicative stress and found that it coordinates the deposition of two histone marks on histone H3, T45 phosphorylation and K56 acetylation. Mechanistically, Pkc1 controls H3K56 acetylation through phosphorylation-dependent activation of Rtt109. Moreover, Pkc1 also promotes H3T45 phosphorylation and this modification is important for the coordinated deposition of H3K56 acetylation.
To examine whether Pkc1 has a role in protecting cells from replicative stress we first determined cell sensitivity to hydroxyurea (HU) in the presence and absence of active Pkc1. To do this we made use of strains containing temperature sensitive pkc1 alleles which could be inactivated by incubating yeast cells at 34ºC, thereby negating the confounding effects of heat shock stress response obtained using the traditionally used pkc1ts allele (Anastasia et al., 2012). In comparison to cells containing wild-type PKC1 (DK186), temperature sensitive pkc1-14 (DK1690) cells, grown at 34ºC to inactivate Pkc1 were inviable (Figure 1A, top panel) consistent with previous studies using a pkc1Δ deletion strain (Queralt and Igual, 2005). Loss of PKC1 function results in cell lysis in the absence of osmotic support. The presence of sorbitol saved the pkc1-14 mutant phenotype (Figure 1A, middle panel) but HU sensitivity was observed under these conditions (Figure 1A, bottom panel). This suggested a role for Pkc1 other than signalling through the well studied downstream MAP kinase cascade which includes the kinase Bck1 (Lee et al., 1993). Indeed bck1Δ (Y01328) cells were not sensitive to HU, further emphasising a new alternative mechanism of action for Pkc1 under replicative stress conditions (Figure 1A). To determine whether the kinase activity of Pkc1 is needed to protect cells from replicative stress, we attempted to rescue pkc1-14 cells by re-expressing a catalytically dead (KD) version of Pkc1 in the presence of HU. However, while wild-type (WT) Pkc1 was able to rescue the growth defect of pkc1-14 cells, the catalytically dead version was unable to do so (Figure 1B).
Together, these experiments demonstrate that Pkc1 kinase activity is required for protecting cells from replicative stress.
During S phase, acetylation of histone H3 at lysine 56 (H3K56) is an important modification in ensuring the correct assembly of newly synthesised nucleosomes and their incorporation into chromatin (Li et al., 2008; Masumoto et al., 2005). We therefore examined whether Pkc1 is required for H3K56 acetylation under replicative stress conditions. Upon release from a HU block, wild-type cells showed accumulation of acetylated H3K56 (Figure 2A, lanes 1–6). In contrast, temperature sensitive pkc1-21 cells (DK1697) showed much reduced levels of H3K56 acetylation when grown at 34ºC (Figure 2A, lanes 7–12) even though they had progressed through S phase and arrested at G2 (Figure 2—figure supplement 1A). Importantly, this effect was not limited to strains harbouring the pkc1-21 allele but was also observed in a different temperature sensitive strain, harbouring the pkc1-14 allele either upon release from a HU block (Figure 2B and Figure 2—figure supplement 1B) or upon entry into S phase upon release from an α factor block (Figure 2—figure supplement 2). H3K56 acetylation is deposited by Rtt109, and this enzyme also acetylates histone H3 at lysine 9 (H3K9) (Driscoll et al., 2007; Han et al., 2007; Tsubota et al., 2007). Interestingly, in addition to H3K56 acetylation defects, pkc1-14 cells showed much reduced levels of H3K9 acetylation (Figure 2B, middle panel) which suggests a defect in Rtt109 activity.
To further explore the role of Pkc1 in promoting H3K56 acetylation, we treated wild-type cells with the Pkc1 inhibitor cercosporamide (Sussman et al., 2004) and examined H3K56 acetylation following release from a HU block. Pharmacological inhibition of Pkc1 activity reduced H3K56 acetylation but importantly did not affect another histone H3 acetylation event at lysine 18 (H3K18) (Figure 2C), demonstrating the specificity of action of Pkc1. We also examined whether combinatorial genetic inactivation and pharmacological inhibition of Pkc1 has additive effects but none were observed, indicating that the effect of cercosporamide was through Pkc1 (Figure 2—figure supplement 3). We note however that the loss of Pkc1 in the temperature sensitive strains resulted in stronger reductions in H3K56ac levels than following cercosporamide treatment, most likely due to incomplete Pkc1 inhibition in the latter case.
Together these results demonstrate an important role for Pkc1 in promoting H3K56 acetylation under replicative stress conditions.
The reduced levels of H3K9 and H3K56 acetylation observed upon disruption of Pkc1 activity suggested that the acetyltransferase Rtt109 which deposits these modifications might be targeted by Pkc1. One mechanism might be through Pkc1-mediated control of Rtt109 phosphorylation. Inspection of Rtt109 sequence revealed a potential phosphorylation site at threonine 46 (T46) which partially matches the PKC consensus sequence (Figure 3A). To establish whether Rtt109 can be phosphorylated by Pkc1 at this site, we raised a phospho-specific antibody which specifically recognised a phosphorylated peptide surrounding T46 (Figure 3—figure supplement 1A). In vitro kinase assays revealed that Pkc1 was able to phosphorylate Rtt109 in the context of a purified recombinant Rtt109-Vps75 heterodimer (Figure 3B). This phosphorylation was reduced to background levels following treatment with lambda phosphatase (Figure 3B, lane 3). Immunoprecipitation assays demonstrated that Pkc1 and Rtt109 could interact in vitro (Figure 3—figure supplement 1B).
Rtt109 phosphorylation at T46 could also be identified in vivo following HU treatment (Figure 3C, lanes 1–4). This phosphorylation was subsequently lost upon release from HU arrest (Figure 3C, lanes 5–6). Importantly, treatment of cells with cercosporamide prior to HU addition blocked the appearance of Rtt109 phosphorylation at T46 (Figure 3D), demonstrating a role for Pkc1 in promoting Rtt109 phosphorylation under replicative stress conditions.
These data therefore indicate that in cells undergoing replicative stress, Rtt109 is phosphorylated in a Pkc1-dependent manner, most likely through direct phosphorylation by Pkc1.
To establish whether phosphorylation of Rtt109 at T46 has any functional relevance, we attempted to rescue rtt109Δ cells lacking Rtt109 protein expression with plasmids encoding mutant proteins which had either lost the ability to be phosphorylated (T46A) or carried a phosphomimetic residue in place of T45 (T46D). In the presence of HU, rtt109Δ cells grew poorly (Figure 4A). This growth defect could be rescued by re-expressing wild-type Rtt109 but this rescue was lost with the non-phosphorylatable Rtt109(T46A) mutant. In contrast, Rtt109(T46D) was able to suppress the growth defect (Figure 4A) indicating that the insertion of a phosphomimetic residue at the Pkc1 phosphorylation site reinstated the activity of Rtt109. To understand the molecular basis to this rescue, we again examined H3K56 acetylation. As expected, this was severely depleted in rtt109Δ cells but restored upon re-expression of wild-type Rtt109 (Figure 4B, left panels). Rtt109(T46A) only partially re-constituted H3K56 acetylation levels and the kinetics were delayed whereas Rtt109(T46D) promoted rapid and high levels of H3K56 acetylation (Figure 4B, left panels). These results are fully consistent with a role for Pkc1-mediated phosphorylation of Rtt109 being required for its ability to promote H3K56 acetylation.
Interestingly, we found that while wild-type Rtt109 and Rtt109(T46D) were inducibly expressed to high levels upon release from HU arrest, Rtt109(T46A) expression was delayed and reached a lower level (Figure 4B, right hand panels). This suggested that Pkc1-mediated phosphorylation might be important for stabilising Rtt109. Indeed, we found that Rtt109 protein was expressed to lower levels in pkc1-14 cells (Figure 4C). This was not due to a transcriptional defect as RTT109 mRNA levels were elevated in pkc1-14 cells (Figure 4—figure supplement 1), suggesting that these cells are attempting to compensate for the loss in Rtt109 protein. This lower level of Rtt109 likely contributes to the decreased levels of H3K56 acetylation we observed. Together, these results indicated that Pkc1-mediated Rtt109 phosphorylation is required for maintaining Rtt109 protein levels and H3K56 acetylation levels as cells recover from HU-mediated replicative stress.
One prediction from these results is that any chromatin changes should manifest themselves in similar gene expression profiles when pkc1-14 cells or cells expressing Rtt109(T46A) are compared. Indeed, in cells released from HU block, a highly significant overlap was observed for pkc1-14 cells or cells expressing Rtt109(T46A) in genes either downregulated or upregulated compared to wild-type cells (Figure 5A and B). Importantly no such significant overlaps were observed when we considered genes that were regulated in opposite directions in the two mutant strains (Figure 5B). Furthermore, when we considered the biological processes that were affected, there was a strong overlap in gene ontology (GO) terms, with over-representation of GO terms associated with processes such as 'cell wall' and 'nucleolus' strongly associated with both mutant strains and reciprocal effects seen with 'telomere maintenance' and 'DNA helicase activity' (Figure 5C and D). These data therefore further support a strong association between Pkc1 activity, phosphorylation of Rtt109 at T46 and subsequent downstream effects on chromatin structure and gene expression. One prediction of these results is that the phosphomimetic version of Rtt109 might be able to bypass the defects seen in pkc1-14 cells. However, no rescue of the growth defects of pkc1-14 cells in the presence of HU was observed (Figure 5—figure supplement 1). Therefore a working model suggested a direct role for Pkc1 in controlling H3K56 acetylation through Rtt109 phosphorylation but also that additional activities were controlled by Pkc1 (Figure 5E).
Previous results in mammalian cells had shown that PKCδ phosphorylates histone H3 at threonine 45 (H3T45) (Hurd et al., 2009). As this residue is conserved in S. cerevisiae (Figure 6A), and is located in the vicinity of K56, it is possible that Pkc1-mediated phosphorylation of T45 might influence acetylation events at K56. We therefore examined whether Pkc1 could phosphorylate H3T45 in vitro by using purified histone H3 and immunoprecipitated Pkc1p. Efficient H3 phosphorylation was observed (Figure 6B) and phosphorylation at T45 was confirmed as the only major phosphorylation site by mass spectrometry (Figure 6C). Next we examined H3T45 phosphorylation and its dependence on Pkc1 in vivo. As cells were released from a HU-mediated block, H3T45 became strongly phosphorylated in wild-type cells (Figure 6D). However, no such rises in H3T45 phosphorylation were observed upon ablation of Pkc1 activity in pkc1-14 cells (Figure 6D, lanes 7–12).
The results therefore indicate that Pkc1 mediates histone H3 phosphorylation under conditions of replicative stress.
Given the relatively close proximity of T45 to K56 in the histone H3 tail and the link of modifications of both of these residues to Pkc1 activity, we next asked whether there is a connection between H3T45 phosphorylation and H3K56 acetylation. Importantly, H3K56 acetylation was greatly reduced in cells containing the H3(T45A) allele upon release from a HU block (Figure 7A, lanes 3 and 4; Figure 7B). In contrast only a small change in H3K56 acetylation was observed upon release from an alpha factor block under normal growth conditions (Figure 7A, lanes 1 and 2). However, if cells were released from an alpha factor-mediated G1 arrest into HU, and the HU subsequently removed, H3K56 acetylation again accumulated following HU removal in wild-type cells. This accumulation of H3K56 acetylation did not occur in cells harbouring the H3(T45A) mutant allele (Figure 7—figure supplement 1). These results suggested functional coupling between H3T45 phosphorylation and H3K56 acetylation. To further probe this phenomenon, we asked whether the Rtt109(T46D) mutant could bypass the defects seen in cells containing the H3(T45A) mutant allele. However, only low levels of H3K56 acetylation were observed in the presence of Rtt109(T46D) (Figure 7C), demonstrating that this protein was unable to suppress the defects from H3(T45A) thereby indicating a crucial role for H3T45 phosphorylation in promoting H3K56 acetylation. Indeed, the Rtt109(T46D) mutant couldn’t bypass the growth defects of H3(T45A) cells in HU (Figure 7D). We also tested whether Rtt109(D) could bypass the HU-mediated growth defects of H3(K56R) cells but again this mutant was unable to rescue the growth defect (Figure 7—figure supplement 2). This further emphasises the importance of Rtt109-mediated H3K56 acetylation in protecting against the deleterious effects of HU treatment, even when H3T45 was available for phosphorylation. These results lead to a model whereby Pkc1 signals through promoting phosphorylation of both Rtt109 and histone H3 to promote H3K56 acetylation. One prediction from this model is that downstream gene expression defects should be at least partially shared by pkc1-14, H3(T45A) and Rtt109(T46A) mutant strains. Indeed pairwise comparisons of the gene expression changes observed between these strains following release from a HU block, showed highly significant overlaps in genes being upregulated in all cases (Figure 7E; Figure 5B). Importantly though, of the 51 genes showing significant upregulation in the pkc1 and Rtt109(T46A) mutant strains, 41 (80%) were also upregulated in the H3(T45A) mutant strain (Figure 7E). Moreover, consistent co-regulation of genes in the 'DNA helicase' GO term category could be observed across all mutant strains (Figure 7F). These mutants therefore exhibit common defects in gene expression. This finding provides further supporting evidence that adds to the mechanistic links we have uncovered between Pkc1, Rtt109, H3T45 phosphorylation and H3K56 acetylation, and strengthens our major discovery that Pkc1 coordinates histone H3 modifications during the replicative stress response.
Newly synthesised DNA must be efficiently packaged into chromatin to ensure that genome stability is maintained (reviewed in Groth et al., 2007; Ransom et al., 2010; Gurard-Levin et al., 2014). This must be achieved during both an unperturbed cell cycle and under conditions of replicative stress where the cell cycle must pause to ensure DNA repair occurs before recommencing the cycle. Histone H3 T45 phosphorylation and K56 acetylation are two important modifications that occur during S phase and are required for chromatin reassembly (Masumoto et al., 2005; Hyland et al., 2005; Ozdemir et al., 2005; Li et al., 2008; Baker et al., 2010). Here we show that in S. cerevisiae Pkc1 coordinates the deposition of these two marks under conditions of replicative stress. Previous results had implicated the Cdc7/Dbf4 kinase complex in directly controlling H3T45 phosphorylation during S phase of and unperturbed cell cycle but this kinase did not affect H3K56 acetylation (Baker et al., 2010). However, a role under replicative stress conditions was not investigated and residual levels of H3T45 phosphorylation were observed in the cdc7Δ cells, indicating the existence of another H3T45 kinase. Indeed, Cdc7/Dbf4 activity has been shown to be downregulated under conditions of replicative stress, suggesting the involvement of a different histone H3 kinase under these conditions (Weinreich and Stillman, 1999). Here we focussed on histone H3 modification control under replicative stress conditions and in combination with previous results (Baker et al., 2010) our data indicate that at least two different kinases are involved in controlling H3T45 phosphorylation, with Cdc7/Dbf4 operating during an unperturbed cell cycle and Pkc1 operating under replicative stress conditions where it also coordinates H3K56 acetylation.
In cells subjected to replicative stress it is important to delay chromatin re-assembly until the DNA damage has been resolved, and one way to do this, would be to delay H3 modifications. It is not clear how this delay is achieved but Pkc1 promotes the re-activation of H3 modifications upon removal of replicative stress. Mechanistically, Pkc1 controls H3K56 acetylation through phosphorylating the Rtt109 acetyltransferase. This modification stabilises Rtt109 and thus safeguards its availability for modifying histone H3 following removal of replicative stress and re-entry into the cell cycle. It is possible that Pkc1 might also directly target other elements of the chromatin modification machinery to coordinate efficient chromatin assembly such as occurs with Cdc7-Dbf4 which can also target CAF-1 (chromatin assembly factor 1) (Gerard et al., 2006) as well as H3T45.
Under replicative stress conditions, H3T45 phosphorylation appears to be critically important for coupled H3K56 acetylation, and this phosphorylation event is promoted by Pkc1-mediated H3T45 phosphorylation. This is likely direct as histone H3 is efficiently phosphorylated at this site in vitro. This leads to a model (Figure 7G) whereby Pkc1 promotes the coordinate modification of these two chromatin modifications and H3T45 phosphorylation is functionally coupled to H3K56 acetylation. It is not clear how this coupling is mediated but one possibility is that H3T45 phosphorylation blocks histone binding to DNA, thus allowing H3K56 to be exposed and hence accessible for acetylation by Rtt109. More complex scenarios are of course possible such as phosphorylated H3T45 acting as binding platform for recruiting a histone mark reader which then coordinates H3K56 acetylation. Indeed, further complexities in the regulatory links between histone H3 modifications and Pkc1-mediated signalling are suggested by the observation that Pkc1 levels appear to be reduced in H3(T45A) mutant strains (data not shown). Future studies will be required to distinguish between and dissect out the underlying molecular mechanisms. One outcome of perturbed Pkc1 activity and its downstream molecular events following HU treatment is likely to be defective chromatin assembly and preliminary evidence suggest that this might be the case (data not shown). Our data show a consistent sensitivity of pkc1, rtt109(T46A) and H3(T45A) mutants to HU treatment which complements previous data showing that H3(K56A) alleles are also sensitive to HU (Matsubara et al., 2007) and supports a role in replicative stress. However, the response to other stress inducing conditions such as MMS and 6-AU treatment is less clear. For example, the H3(T45A) strain is sensitive to both MMS and 6-AU whereas the rtt109(T46A) strain is only sensitive to MMS (data not shown). Thus different elements of the regulatory network we have uncovered may be used to respond to different stress inducers.
Another open question is how Pkc1 is specifically targeted/activated during replicative stress but one potential regulator would the cell cycle-dependent changes of the cell wall and signalling through the cell wall integrity pathway. Alternatively, other upstream pathways might be involved, and one potential link might be through TOR signalling as TORC2 has been shown to phosphorylate and activate Pkc1 (Nomura and Inoue, 2015) and TOR signalling has also been linked to H3K56 acetylation (Chen et al., 2012). Whether such a route operates during replicative stress or is employed under different conditions is not clear but re-emphasises the potential widespread importance of connections between Pkc1 and histone H3 modifications we have identified.
Our work may be directly relevant to mammalian systems where PKCδ has been shown to phosphorylate H3T45 under apoptotic conditions (Hurd et al., 2009), although others have identified AKT as an additional H3T45 kinase under DNA damage conditions (Lee et al., 2015). It is not clear whether PKC is linked to H3K56 acetylation in mammals but this modification is induced by replicative stress and its deposition is mediated by p300/CBP, the functional homologue of Rtt109 (Das et al., 2009; Vempati et al., 2010). Indeed, atypical protein kinase C zeta has been shown to phosphorylate and control the histone acetylation output of CBP in the context of neuronal differentiation (Wang et al., 2010). Given these links between PKC and CBP/p300 it appears likely that our findings will be directly relevant to human cancer where oncogene-driven replicative stress is an important contributory event (Bartek et al., 2007) and therefore warrants further investigation.
For bacterial expression: pET28a+His-Rtt109 and pET3a-Tr-Vps75-Flag was kindly provided by Paul Kaufman (Tsubota et al., 2007).
For yeast expression; pVD67 (pAS1995; encoding wild-type GFP-Pkc1), pVD61(empty vector) and pVD124 (kinase-dead GFP-Pkc1) were kindly provided by Martha Cyert (Denis and Cyert, 2005). pAS4104 encoding Myc-Rtt109, pAS4105 (myc-Rtt109T46A) and pAS4106 (myc-Rtt109T46D) were made by inserting BamHI-cleaved PCR product generated using the templates pAS4101-4103 and primer pair ADS4723/4724 into pCM188. pAS4101 was generated by amplifying the cDNA sequence of the RTT109 gene encoding full-length protein from yeast genomic DNA and cloning into pGBKT7 using EcoRI/SalI restriction sites. pAS4102 (encoding Rtt109[T45A]) and pAS4103 (encoding Rtt109[T45D]) were created by QuikChange mutagenesis (Stratagene) using the primer pairs ADS4727/ADS4728, ADS4729/4730 and pAS4101 as a template. pGal1Vps75-flag was kindly provided by Tom Owen-Hughes.
His-tagged fusion proteins were prepared using Ni-NTA agarose beads (Qiagen, UK) according to the manufacturer’s protocol and HA epitope-tagged Pkc1p were prepared essentially as described previously (Darieva et al., 2012). Recombinant histone H3 was made commercially (Active Motif).
Pulldown assays with immunoprecipitated HA-Pkc1 from DZ2 cells and in vitro translated Rtt109 were carried out as described previously (Pic-Taylor et al., 2004).
To detect epitope-tagged derivatives by western analysis, anti-HA (Roche) and anti-tubulin TAT-1 (CRUK), H3K56ac (Active motif), H3K9ac (Abcam), H3K18ac (Abcam), H3 (Active motif), myc epitope (Santa Cruz), H3T45-P (Active motif) antibodies and Supersignal west dura substrate (Pierce) were used.
Rabbit polyclonal monospecific antibody against the phosphothreonine at amino acid 46 on yeast Rtt109 (Rtt109 T46-P) was generated from immunizing rabbits with a KLH-conjugated peptide (H-DDKRVPKST(PO3H2)IKTC-NH2), and then cross-affinity purification of polyclonal antibodies specific to modified Rtt109 T46-P or non-modified pRtt109 was performed (Eurogentec). For immunoblotting analysis we used 1:1000 dilution for both antibodies.
Protein kinase assays were performed as described previously (Darieva et al., 2012) using HA epitope-tagged Pkc1p immunoprecipitated form DZ2 cells and recombinant H3 or His-Rtt109/Vps75 protein complex as a substrate.
Proteins were isolated from denaturing polyacrylamide gels and proteolytically digested as described previously (Darieva et al., 2012). Digested samples were analysed by LC-MS/MS using an UltiMate® 3000 Rapid Separation LC (RSLC, Dionex Corporation, Sunnyvale, CA) coupled to an Orbitrap Elite (Thermo Fisher Scientific, Waltham, MA) mass spectrometer. Peptide mixtures were separated using a gradient from 92% A (0.1% FA in water) and 8% B (0.1% FA in acetonitrile) to 33% B, in 44 min at 300 nl min-1, using a 75 mm x 250 μm i.d. 1.7 μM BEH C18, analytical column (Waters). Peptides were selected for fragmentation automatically by data dependant analysis. Data were analysed as described previously (Darieva et al., 2012) but phosphorylated peptide product ion spectra were also manually validated.
The yeast strains used are listed in Supplementary file 1. Yeast cells were grown, GAL1-promoter-driven constructs induced, and transformations performed, as described previously (Darieva et al., 2003). Yeast cultures were treated with 40 nM cercosporamide (Santa Cruz) to inhibit PKC activity, synchronised in G1 phase by treatment with α-factor and in early S phase by hydroxyurea (HU) treatment as described previously (Darieva et al., 2012). DNA content analyses were performed using propidium iodide-stained cells as described previously (Darieva et al., 2006).
Growth sensitivity tests were performed as described previously (Darieva et al., 2012) except that temperature sensitive pkc mutant strains were grown at 30°C or 34°C. The experiments were performed in duplicate and were repeated several times.
RNA extraction and real-time reverse transcription-PCR (RT-PCR) analysis were carried out as described previously (Darieva et al., 2006) using the following primer pairs: ADS1587/1588 (HTT2), ADS4732/4733 (RTT109) and ADS3978-ADS3979 (18S rRNA). All data were normalized to 18S rRNA levels in the same cells.
Duplicate RNA samples were labelled and hybridised after a single round of amplification to Affymetrix Yeast Genome 2.0 array chips. All the cell strains used in this experiment were grown in the presence of 1M sorbitol at 30ºC, treated with 200 mM HU for 2.5 hr and released from HU block at 34ºC. Samples were obtained from: (1) Wild-type (DK186) and pkc1-14 (DK1690) mutant strains. The mRNA expression data were collected at three timepoints (wild-type blocked in HU, wild-type and pkc1-14 released from HU block for 75 min. (2) rtt109Δ mutant cells (Y01490) containing vectors expressing wild-type (WT) Rtt109 (DZ5) or Rtt109(T46A) (DZ6). The mRNA expression data were collected at three timepoints (WT blocked in HU, and Rtt109(WT) and Rtt109(T46A) released from HU block for 80 min. (3) Wild-type cells (MSY748) expressing wild-type histone H3 (MSY748) or H3(T45A) (H3-T45A). The mRNA expression data were collected at three timepoints (WT blocked in HU, and both WT and H3(T45A) released from HU block for 80 min). The measurements were then normalised by using robust multi-array average (RMA) approach (Irizarry et al., 2003). The primary data are deposited in ArrayExpress; accession number E-MTAB-3359.
Limma (Smyth, 2005) was used to provide log(foldChange), T-statistic and P-values. In all cases, fold changes were calculated relative to the wild-type strain released from HU block. Individual gene expression changes were considered significant if they satisfied the criteria of fold change >1.2 and P-value <0.05. The T-statistics, P-values and Fold Changes were used by Piano (Väremo et al., 2013) to build aggregate P-values for all of the genes in each GO term, based upon the values of these statistics for each of the probe sets matching to the term. GO terms were then ranked separately by aggregate P-values, within each of the five P-value directional classes (upregulated or downregulated in both conditions), and this ranking provided a consensus score.
To create heatmaps of expression changes of genes within GO terms z-scores were calculated for the set of arrays testing a pair of strains across different conditions and an average z-score for each condition determined. Each paired experiment was treated independently and the z-scores were clustered and depicted in a heatmap using pheatmap (http://cran.r-project.org/web/packages/pheatmap/index.html).
Control of mitotic events by Nap1 and the Gin4 kinaseThe Journal of Cell Biology 138:119–130.https://doi.org/10.1083/jcb.138.1.119
A link between mitotic entry and membrane growth suggests a novel model for cell size controlThe Journal of Cell Biology 197:89–104.https://doi.org/10.1083/jcb.201108108
Protein kinase C regulates late cell cycle-dependent gene expressionMolecular and Cellular Biology 32:4651–4661.https://doi.org/10.1128/MCB.06000-11
Histone chaperones: assisting histone traffic and nucleosome dynamicsAnnual Review of Biochemistry 83:487–517.https://doi.org/10.1146/annurev-biochem-060713-035536
The replication kinase Cdc7-Dbf4 promotes the interaction of the p150 subunit of chromatin assembly factor 1 with proliferating cell nuclear antigenEMBO Rep 8:817–823.
Phosphorylation of histone H3 thr-45 is linked to apoptosisThe Journal of Biological Chemistry 284:16575–16583.https://doi.org/10.1074/jbc.M109.005421
Insights into the role of histone H3 and histone H4 core modifiable residues in saccharomyces cerevisiaeMolecular and Cellular Biology 25:10060–10070.https://doi.org/10.1128/MCB.25.22.10060-10070.2005
A yeast mitogen-activated protein kinase homolog (mpk1p) mediates signalling by protein kinase cMolecular and Cellular Biology 13:3067–3075.https://doi.org/10.1128/MCB.13.5.3067
AKT phosphorylates H3-threonine 45 to facilitate termination of gene transcription in response to DNA damageNucleic Acids Research 43:4505–4516.https://doi.org/10.1093/nar/gkv176
Cell wall integrity signaling in saccharomyces cerevisiaeMicrobiology and Molecular Biology Reviews 69:262–291.https://doi.org/10.1128/MMBR.69.2.262-291.2005
Methylglyoxal activates the target of rapamycin complex 2-protein kinase c signaling pathway in saccharomyces cerevisiaeMolecular and Cellular Biology 35:1269–1280.https://doi.org/10.1128/MCB.01118-14
Characterization of lysine 56 of histone H3 as an acetylation site in saccharomyces cerevisiaeThe Journal of Biological Chemistry 280:25949–25952.https://doi.org/10.1074/jbc.C500181200
P300-mediated acetylation of histone H3 lysine 56 functions in DNA damage response in mammalsThe Journal of Biological Chemistry 285:28553–28564.https://doi.org/10.1074/jbc.M110.149393
Jessica TylerReviewing Editor; University of Texas MD Anderson Cancer Center, United States
eLife posts the editorial decision letter and author response on a selection of the published articles (subject to the approval of the authors). An edited version of the letter sent to the authors after peer review is shown, indicating the substantive concerns or comments; minor concerns are not usually shown. Reviewers have the opportunity to discuss the decision before the letter is sent (see review process). Similarly, the author response typically shows only responses to the major concerns raised by the reviewers.
Thank you for submitting your work entitled "Protein kinase C coordinates histone H3 phosphorylation and acetylation" for peer review at eLife. Your submission has been favorably evaluated by Jim Kadonaga (Senior editor), and two reviewers, one of whom is a member of our Board of Reviewing Editors.
The reviewers have discussed the reviews with one another and the Reviewing editor has drafted this decision to help you prepare a revised submission. The reviewers felt that your work was interesting, important and timely.
1) All MNase analyses have to be removed from the paper.
2) Show the effect on H3 K56Ac of Pkc inhibition in the pkc1-14 and pkc1-21 mutants, to establish whether they are epistatic, given their different effect on the kinetics of H3 K56Ac.
3) Indicate concentrations of HU and temperatures used for all experiments.
4) Include H3K56A or R mutants in the epistasis analysis in Figure 7D to enable a better understanding of the codependence of H3 K56Ac, H3 T64p and Rtt109p.
5) Make the gene expression data publicly available.
6) Please show or report result of sensitivities of the pkc1, H3T45 and Rtt109T46 alleles to MMS and 6AU.
We have included the full reviews for your benefit below.
Reviewer #1: This is an interesting paper that shows that Pkc1 phosphorylates the HAT Rtt109 to stabilize Rtt109 after release from HU treatment, enabling it to acetylate H3 K56 during S phase. They also show that Pkc1 promotes phosphorylation of H3 T45, which also promotes H3 K56Ac. The findings are novel and important. Some of the westerns are rather ugly but are still convincing. The micrococcal nuclease analyses however are not technically acceptable nor credible, nor consistent with data published for the same mutants in the literature previously. Additionally, they do not add anything to the story and need to be removed, given the effect of lack of H3 K56Ac on chromatin and MNase ladders is well established already.
For future reference this is what is wrong with the MNase ladders (probably irrelevant here, because these all have to be removed from the paper), MNase accessibility analysis requires multiple time points or different MNase concentrations to be shown for each chromatin sample, not just one. The gel percentages and ladders need to be used that will enable 150bp and 300bp fragments to be seen. Also, the same amount of sample has to be digested for different samples. This is clearly not the case in these MNase analyses, especially Figure 2 and Figure 5. For the MNase digestion in Figure 1, the wild type sample is totally unconvincing. It is not a MNase ladder – there are intermediate bands between the nucleosomes and the tri-nucleosome is far brighter than the mono and di bands, but there is no tetra. This does not happen; they never look like this; and the state of overdigestion of the Pkc1 mutant would not be compatible with life! The results of the MNase digestion analysis of H3 K56R is far more extreme than what Steve Jackson published in Nature nearly ten years ago, and this degree of relative lack of chromatin would not support life. Figure 2 MNase also lacks a ladder. The labeling of the size markers on Figure 5 does not correspond to the size markers and appears to be completely arbitrary. Where is the mononucleosome on this analysis? A ladder cannot have di and tri and no mononucleosome.
Reviewer #2: Sharrocks and colleagues present a number of interesting experiments that implicate the yeast PKC kinase in chromatin assembly regulation. Yeast growth phenotypes are applied to implicate pkc1 alleles in HU resistance and H3K56 acetylation as well as to study H3 and Rtt109 PKC phosphorylation site mutant alleles. Co-IP and in vitro and in vivo phosphorylation assays suggest a network of phosphorylation whereby PKC may directly phosphorylate H3 on T45 and Rtt109 on T46. The emergent model is attractive because there appears to be a two-pronged PKC-mediated regulatory pathway, whereby direct PKC phosphorylation of H3T45 promotes acetylation of H3K56 whilst concomitant phosphorylation of the H3K56 acetylase Rtt109 on T46 also promotes H3K56 acetylation. The model appears to have been tested adequately to sustain the claims, including epistasis experiments using colony formation and gene expression as a read-outs.
The MNase experiments are scattered over three figures (1C, 2C, 6E), assessing MNase patterns in wild type, pkc1-14 (1C) H3K56R (2C) and a H3T45A mutant +/- PKC1 expression (6E). It is not clear whether as such these add substantially to the manuscript. Technically a full titration/time course of MNase for each strain/condition would be more informative. Furthermore, as different strain backgrounds are compared (1C = W303, 2C = Rothstein 1983 = W303, 6E = FY406), at different temperatures (30ºC? and 34ºC), the only result is that the chromatin structure is MNase hypersensitive and therefore 'messed up'. The assays displayed do not have the resolution to indicate in what way or to what extent the chromatin is messed up. Darieva et al. again do not systematically indicate at what temperature the control strain was grown. I feel that one dedicated accessibility figure describing a logical set, rather than the present chronological-discovery-set across three figures would render the manuscript stronger on this front. For instance, comparing WT, H3T45A and H3T45D combined with pkc mutants at 34ºC. A further set of possibilities would include the H3K56A, and R mutations. Yet another, would employ the rtt109 null and Rtt109A and Rtt109D alleles. Leaving MNase experiments out altogether is also an option, however.https://doi.org/10.7554/eLife.09886.019
- Andrew D Sharrocks
- Andrew D Sharrocks
- Andrew D Sharrocks
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
We are grateful to Karren Palmer for technical support, and to staff in the core genomics technologies, mass spectrometry, bioinformatics, and flow cytometry facilities. We also thank Catherine Millar, Alan Whitmarsh, Iain Hagan and members of our laboratories for comments on the manuscript and helpful discussions, and Catherine Millar, Martha Cyert, Paul Kaufman, Tom Owen-Hughes, and Doug Kellog for reagents. This work was supported by the BBSRC, the Wellcome Trust and a Royal Society-Wolfson award to ADS.
- Jessica Tyler, Reviewing Editor, University of Texas MD Anderson Cancer Center, United States
© 2015, Darieva et al.
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.