Strain, strain background (Mus Musculus) | Tg(Htr3a-EGFP)DH30Gsat (referred as Htr3a-GFP) | GENSAT Consortium | MGI:3846657 | Maintained on a C57Bl/6 background |
Strain, strain background (Mus Musculus) | B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J (referred as R26R-tdTOMfl/fl) | The Jackson Laboratory | MGI:104735 | Maintained on a C57Bl/6 background |
Strain, strain background (Mus Musculus) | Tg(Hmx3-icre)1Kess | provided by Oscar Marin | MGI:5566775 | Maintained on a C57Bl/6 background |
Strain, strain background (Mus Musculus) | Dbx1tm2(cre)Apie (referred as Dbx1-Cre) | provided by Alessandra Pierani | MGI:3757955 | Maintained on a C57Bl/6 background |
Antibody | Anti-GFAP, rabbit polyclonal | Abcam, United Kingdom | ab7260 | (1:2000) |
Antibody | Anti-GFP, rabbit polyclonal | Millipore, Germany | AB3080 | (1:500) |
Antibody | Anti-GFP, goat polyclonal | Abcam | ab5450 | (1:2000) |
Antibody | Anti-NeuN (clone A60), mouse monoclonal | Millipore | MAB377 | (1:500) |
Antibody | Anti-NKX2.1 (H-190), rabbit polyclonal | Santa Cruz Biotechnology, Dallas, TX | sc-13040 | (1:100) |
Antibody | Anti-NPY, rabbit polyconal | Abcam | ab10980 | (1:500) |
Antibody | Anti-NR2F2, rabbit polyclonal | Abcam | ab42672 | (1:500) antigen retrieval (Citrate buffer pH 6.0; 85°C; 20 min) |
Antibody | Anti-PROX1, goat polyclonal | R&D System,Minneapolis, MN | AF2727 | (1:250) |
Antibody | Anti-PV, mouse monoclonal | Swant, Switzerland | PV235 | (1:2000) |
Antibody | Anti-Reelin, mouse monoclonal | Abcam | ab78540 | (1:500) |
Antibody | Anti-S100β, rabbit polyclonal | Abcam | ab41548 | (1:2000) |
Antibody | Anti-SST, rat monoclonal | Millipore | MAB354 | (1:500) |
Antibody | Anti-SOX6, rabbit polyclonal | Abcam | ab30455 | (1:500) |
Antibody | Anti-SOX10 (N-20), goat polyclonal | Santa Cruz Biotechnology | sc-17343 | (1:100) |
Antibody | Anti-SP8 (C-18), goat polyclonal | Santa Cruz Biotechnology | sc-104661 | (1:50) antigen retrieval (Citrate buffer pH 6.0; 85°C; 20 min) |
Antibody | Anti-tdTOM, goat polyclonal | Sicgen, Portugal | AB8181-200 | (1:500) |
Antibody | Anti-VIP, rabbit polyclonal | Abcam | ab22736 | (1:500) ASCF perfusion; 2 hr PFA 4% postfixation |
Antibody | Donkey anti-rabbit Alexa Fluor488 | Invitrogen, Carlsbad, CA | A21206 | (1:500) |
Antibody | Donkey anti-goat Alexa Fluor488 | Invitrogen | A11055 | (1:500) |
Antibody | Donkey anti-goat Alexa Fluor405 | Abcam | ab175664 | (1:500) |
Antibody | Donkey anti-rabbit Alexa Fluor405 | Abcam | ab175651 | (1:500) |
Antibody | Donkey anti-rabbit Alexa Fluor647 | Invitrogen | A31573 | (1:500) |
Antibody | Donkey anti-rat Alexa Fluor647 | Invitrogen | A21247 | (1:500) |
Antibody | Donkey anti-mouse Alexa Fluor647 | Invitrogen | A31571 | (1:500) |
Antibody | Donkey anti-goat Alexa Fluor647 | Invitrogen | A21447 | (1:500) |
Antibody | Streptavidin, Alexa Fluor647-conjugated | ThermoFisher/Invitrogen | S21374 | (1:500) |
Antibody | Anti-Digoxigenin-AP, Fab fragment | Roche, Switzerland | 11082736103 | (1:2000) |
Recombinant DNA reagent | Htr3a plasmid probe | Gift from Dr. B. Emerit | NA | Linearization: HindIII-HF; antisense synthesis: T7; concentration 1 µg |
Recombinant DNA reagent | Lhx6 plasmid probe | Gift from Dr. M. Denaxa | NA | Linearization: Not1; antisense synthesis: T3; concentration 1 µg |
Recombinant DNA reagent | RNAScope Probe-tdTomato-C2 | Affimetrix, Santa Clara, CA | 317041-C2 | |
Recombinant DNA reagent | RNAScope Probe-EGFP | Affimetrix | 400281 | |
Recombinant DNA reagent | RNAScope Probe-Car4-C3 | Affimetrix | 468421-C3 | |
Sequence-based reagent | Genotyping PCR primer forB6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J | Microsynth, Switzerland (desalted;100 µM stock) | WT-F (oIMR9020): AAG GGA GCT GCA GTG GAG TA | https://www2.jax.org/protocolsdb/f?p=116:5:0::NO:5:P5_MASTER_PROTOCOL_ID,P5_JRS_CODE:29436,007909 |
Sequence-based reagent | Genotyping PCR primer forB6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J | Microsynth (desalted;100 µM stock) | WT-R (oIMR9021): CCG AAA ATC TGT GGG AAG TC | |
Sequence-based reagent | Genotyping PCR primer forB6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J | Microsynth (desalted;100 µM stock) | Mut-R (oIMR9103): GGC ATT AAA GCA GCG TAT CC | |
Sequence-based reagent | Genotyping PCR primer forB6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J | Microsynth (desalted;100 µM stock) | Mut-F (oIMR9105): CTG TTC CTG TAC GGC ATG G | |
Sequence-based reagent | Genotyping PCR primer for Tg(Htr3a-EGFP)DH30Gsat | Microsynth (desalted;100 µM stock) | Com-F (273): GCA AGA TGT GAC CAA GCC ACC TAT TT | http://www.med.unc.edu/mmrrc/resources/genotyping-protocols/mmrrc-273 |
Sequence-based reagent | Genotyping PCR primer for Tg(Htr3a-EGFP)DH30Gsat | Microsynth (desalted; 100 µM stock) | WT-R: CAG CCC TCA GCC CTT TGA GAC TTA AG | |
Sequence-based reagent | Genotyping PCR primer for Tg(Htr3a-EGFP)DH30Gsat | Microsynth (desalted; 100 µM stock) | Mut-R: TGA ACT TGT GGC CGT TTA CGT CG | |
Sequence-based reagent | Genotyping PCR primer forTg(Hmx3-icre)1Kess | Microsynth (desalted;100 µM stock) | Mut-F: CTC TGA CAG ATG CCA GGA CA | |
Sequence-based reagent | Genotyping PCR primer forTg(Hmx3-icre)1Kess | Microsynth (desalted;100 µM stock) | Mut-R: TCT CTG CCC AGA GTC ATC CT | |
Sequence-based reagent | Genotyping PCR primer forDbx1tm2(cre)Apie | Microsynth (desalted;100 µM stock) | WT-F (1307): GCA AGG AAA TGT CTC TGG GAC | https://www.infrafrontier.eu/sites/infrafrontier.eu/files/upload/public/pdf/genotype_protocols/EM01924_geno.pdf |
Sequence-based reagent | Genotyping PCR primer forDbx1tm2(cre)Apie | Microsynth (desalted;100 µM stock) | WT-R (1115): GAG GAT GAG GAA ATC ACG GTG | |
Sequence-based reagent | Genotyping PCR primer forDbx1tm2(cre)Apie | Microsynth (desalted;100 µM stock) | Mut-F (cre83): GTC CAA TTT ACT GAC CGT ACA CC | |
Sequence-based reagent | Genotyping PCR primer forDbx1tm2(cre)Apie | Microsynth (desalted;100 µM stock) | Mut-R (cre85): GTT ATT CGG ATC ATC AGC TAC ACC | |
Commercial assay or kit | VECTASTAIN Elite ABC-HRP Kit | VectorLab, Burlingame, CA | PK-6100 | Manufacturer's protocol |
Commercial assay or kit | DAB Peroxidase (HRP) Substrate Kit (with Nickel), 3,3’-diaminobenzidine | VectorLab | SK-4100 | Manufacturer's protocol |
Commercial assay or kit | RNAscope Fluorescence Multiplex Reagent Kit | Advanced Cell Diagnostics, Newark, CA | 320850 | Manufacturer's protocol (fresh frozen tissue) |
Chemical compound, drug | Nε-(+)-Biotinyl-L-lysine (biocytin) | Sigma Aldrich, Germany | B4261 | Used at 8.1 mM |
Chemical compound, drug | Fast Red tablets | Kem-En-Tech, Denmark | 4210 | Manufacturer's protocol |
Chemical compound, drug | Hoechst 33258 | Sigma Aldrich | 23491-45-4 | (1:10000) |
Chemical compound, drug | Thiopental Inresa 0.5 g | Inresa Arzneimittel GmbH, Germany | | Used at 50 mg/kg |
Software, algorithm | Microsoft Office 2017 (Excel, Word) | © 2017 Microsoft, Redmond, WA | v.16.9.1 | Manuscript editing |
Software, algorithm | Adobe Suit CC (Photoshop, Illustrator, Acrobat) | Adobe Systems, San José, CA | v.22.0.1 | Image treatment, figure editing |
Software, algorithm | GraphPad Prism | GraphPad software, Inc., La Jolla, CA | v.7.0 | Statistics, graph editing |
Software, algorithm | Fiji | doi:10.1038/nmeth.2019 | v.2.0.0 | Image editing, manual counting |
Software, algorithm | EndNote X | Thomson Reuters, Canada | v.7.7.1 | Reference editing, bibliography |
Software, algorithm | Neurolucida v.11.02.1 | Microbrightfield, MBF Bioscience, Williston, VT | v.11.02.1 | Neuron reconstruction |
Software, algorithm | Neurolucida Explorer | Microbrightfield, MBF Bioscience | v.11.02.1 | Morphological reconstruction editing |
Software, algorithm | MatLab | MathWorks, Natick, MA | | Electrophysiological recordings measurment/editing |
Software, algorithm | Ephus (MATLAB-based) | doi: 10.3389/fncir.2010.00100 | v. 2.1.0 | Electrophysiological recordings data aquisition |
Software, algorithm | Clampfit | Molecular Devices, San José, CA | v. 10.1.0.10 | Electrophysiological recordings offline analysis |
Software, algorithm | R programming language | www.R-project.org | v. 3.4.0 | Statistics |
Software, algorithm | R package bmrm | doi:10.1038/ncomms14219 | v. 3.5 | Prediction model |
Software, algorithm | NLMorpholoyConverter | http://neuronland.org/NLMorphologyConverter/NLMorphologyConverter.html | v. 0.8.1 | Morphological reconstruction editing |
Software, algorithm | R package NeuroAnatomy Toolbox | doi: 10.1016/j.neuron.2016.06.012 | v. 1.8.12.9000 | Morphological reconstruction editing |