An optimized cytosine base editing system for Leishmania species improves editing efficiency, reduces toxicity, and ensures stable guide expression, enabling scalable loss-of-function screening and efficient creation of functional mutants.
Oversized mammalian cells reduce their growth efficiency by activating global protein degradation, which functions in parallel with the cell size checkpoints, to promote cell size homeostasis.
Ilya A Rybak, Natalia A Shevtsova ... Alain Frigon
Computational modeling based on experimental data reveals how spinal sensorimotor circuits control cycle and phase durations during treadmill locomotion after an incomplete spinal cord injury.
Highly accurate automated cell annotation in multi-cell imaging enables cell identification and reduces subjectivity in Caenorhabditis elegans neural image processing.
NMR and clustering results show that N-terminal acetylation of α-syn enhances its binding to the neutral phospholipid lysophosphatidylcholine, thereby promoting its function of clustering synaptic vesicles.
Mrityunjay Singh, Dinesh C Indurthi ... Shailendra Asthana
MD-simulations reveal low- to high-affinity conformational shift of orthosteric site in the nicotinic acetylcholine receptor, offering insight into agonist roles in activation dynamics, and highlighting the utility of agonist efficiency.
