Development of nanoluciferase complementation-based tau biosensors to detect tau conformational change and oligomerization in living cells in high-throughput assays.
Johannes Krug, Birgit Perner ... Christoph Englert
Using the short-lived turquoise killifish, a transparent in vivo reporter line named klara is established that will be a valuable tool to explore development, senescence, aging, and regeneration.
In the central amygdala, transcriptomic definition of cell types and corresponding spatial transcriptomic analysis reveals major regional differences in molecular organization and relates newly identified molecularly defined cell types to major axon projection targets.
Asgar H Ansari, Manoj Kumar ... Debojyoti Chakraborty
The web-server CriSNPr overcomes difficulties associated with the different CRISPR diagnostic platforms that stem from Cas-specific single guide RNA design parameters, thereby minimizing the time and effort required for individual assay design.
The Brain Extraction Network (BEN) provides a robust, accurate, and generalizable solution not only for extracting brain tissue from multimodal MRI data in rodents, non-human primates, and humans, but also for improving the accuracy of downstream neuroimaging processing tasks.
Valentyna Zinchenko, Johannes Hugger ... Anna Kreshuk
Unsupervised machine learning on the ultrastructure and shape of cells in volume electron microscopy yields a compact representation of cellular morphology that complements genetics-based cell type characterisation.
Bavat Bornstein, Lia Heinemann-Yerushalmi ... Elazar Zelzer
Transcriptomic and proteomic analyses of the murine intact muscle spindle provide comprehensive datasets of its different tissues and identify new tools to study its development and function.
Single-cell sequencing of the alveolar bone marrow of apical periodontitis reveals the cellular and molecular composition of the microenvironment and highlights an osteogenic potential within mesenchymal stem cells of inflammatory-related bone diseases.
DeCalciOn is a low-cost open-source hardware system for real-time in vivo calcium imaging that offers capabilities for online decoding of neural population activity and delivery of short latency closed-loop feedback in freely behaving animals.
