Brooke D Huisman, Zheng Dai ... Michael E Birnbaum
Yeast surface-displayed libraries, when coupled with pooled oligonucleotide synthesis and next-generation sequencing, can be used as a platform to assess binding of whole viral proteomes to class II major histocompatibility complex proteins.
Henriette Hoffmann-Veltung, Nsoh Godwin Anabire ... Maria del Pilar Quintana
An improved 'plug-and-play' reversed FluoroSpot assay is described and validated that allows the simultaneous assessment of antibody cross-reactivity at the single-cell level against up to four variants of a polymorphic antigen of interest.
Lewis Macdonald, Gillian C Taylor ... Andrew J Wood
Auxin-inducible degradation of CRISPR-tagged endogenous proteins in mice reveals cell-type-specific dependence on the mitotic chromosome condensation proteins.
William T Redman, Nora S Wolcott ... Michael J Goard
Two-photon imaging through glass microperiscopes allows imaging of the transverse plane of the hippocampus, including all major subfields, in awake behaving mice.
A new computational approach for detecting sleep waveforms reveals that the 11–15 Hz sleep 'spindle', a neural rhythm implicated in memory consolidation, co-occurs widely across cortex much more often than previously thought.
Laura Medina-Ruiz, Robin Bartolini ... Gerard J Graham
The use of multi-chemokine receptor reporter mice helps to unravel the dynamics of receptor involvement in leukocyte migration in vivo and suggests specificity, rather than redundancy, in receptor use.
Minian is an open-source analysis pipeline for calcium imaging data that enhances usability with low memory demands and transparency with user-friendly interactive visualizations.
Partial response electromyography is a useful and reliable measure of action stopping in a stop signal task, supported by psychometric and meta-analytic evidence.
