Marion Rosello, Juliette Vougny ... Filippo Del Bene
Base editors, with improved specificity and expanded PAM sequence recognition, can be used in zebrafish for the rapid and efficient introduction of single base pair mutations.
Kenichi Shimada, John A Bachman ... Timothy J Mitchison
shinyDepMap helps users explore the essentiality, selectivity, and function of the genes across hundreds of cancer cell lines and identify cancer drug targets.
Combining global and local force measurements of reconstituted muscle tissue using a new chamber system that is compatible with high-resolution microscopy shows mechanical homeostasis already within a week.
Rachel Knoener, Edward Evans III ... Lloyd M Smith
The distinct protein-RNA interactomes of HIV-1 RNA splice forms are revealed using a powerful multiplex strategy for RNA capture and mass spectrometric analysis.
BisMapR reveals strand-specific R-loops with high resolution at small-scale genomic features including bidirectionally transcribed promoters and enhancers.
BLITZ system enables proximity-dependent biotin labelling in live zebrafish embryos with cell and tissue specificity, providing a versatile and valuable tool for proteomic discovery using the zebrafish model.
Rebecca C Adikes, Abraham Q Kohrman ... David Q Matus
During organismal development, CDK activity at mitotic exit is predictive of future cell behavior, indicating whether a cell will divide again or enter a quiescent state.
Alexander J Sercel, Alexander N Patananan ... Michael A Teitell
Applied pressure enables efficient isolated mitochondrial transfer and stable mitochondrial DNA (mtDNA) transplantation into a range of mtDNA-depleted cell types to restore respiration.
Computational models and software connect metagenomics to metabolic network reconstruction, assess metabolic complementarity between species, and identify critical species associated to functions of interest.
