A deep learning-based pipeline was developed for extracting cellular signals flexibly from moving cells in 3D time lapse images, and it outperformed previous methods under different imaging conditions.
David M Young, Siavash Fazel Darbandi ... Stephan J Sanders
A computational tool developed to construct three-dimensional anatomical atlases from two-dimensional data generates volumetrically complete and aligned atlases for all stages of development in the Allen Developing Mouse Brain Atlas.
The response from discrete stages of the early auditory pathway can be measured by subtle manipulations to long-form natural speech stimuli paired with deconvolution analysis of electroencephalography data.
Detection of translated lncRNAs through selective isolation of active ribosomes, ribosome-associated RNAs and corresponding de novo synthesised peptides.
Marion Rosello, Juliette Vougny ... Filippo Del Bene
Base editors, with improved specificity and expanded PAM sequence recognition, can be used in zebrafish for the rapid and efficient introduction of single base pair mutations.
Kenichi Shimada, John A Bachman ... Timothy J Mitchison
shinyDepMap helps users explore the essentiality, selectivity, and function of the genes across hundreds of cancer cell lines and identify cancer drug targets.
Combining global and local force measurements of reconstituted muscle tissue using a new chamber system that is compatible with high-resolution microscopy shows mechanical homeostasis already within a week.
Rachel Knoener, Edward Evans III ... Lloyd M Smith
The distinct protein-RNA interactomes of HIV-1 RNA splice forms are revealed using a powerful multiplex strategy for RNA capture and mass spectrometric analysis.
BisMapR reveals strand-specific R-loops with high resolution at small-scale genomic features including bidirectionally transcribed promoters and enhancers.
