A new system to genetically label and manipulate plasma cells in vivo in their microenvironment resolves current technical limitations and reveals tissue-specific homeostatic population turnover.
Rui Tang, Christopher W Murray ... Monte M Winslow
A novel reporter system that employs cell surface antigen-nanobody pairing enables physical interaction-dependent labeling and functional modification between diverse cell types.
anTraX is an algorithm and software package that facilitates automated analyses of insect social behavior in species and experimental settings that are not accessible with currently existing technology.
Timothy J Duerr, Ester Comellas ... James R Monaghan
Whole mount 3D visualization of macromolecule synthesis with light sheet fluorescence microscopy enables quantitative, multiscale analysis at the organ, cellular, and molecular levels of organization.
New protein labeling strategies unravel the subcellular distribution of neurotransmitter receptor subunits and voltage-gated ion channels in motion-sensing T4/T5 neurons of the Drosophila visual system.
The DIAMonDS can automatically and sequentially identify time points of multiple life cycle events such as pupariation, eclosion, and death in individual flies at high temporal resolution and large scale.
Automated liquid handling, whole mount staining, and clearing allow unbiased 3D quantitation of cell markers in human neural organoids with diameters of up to 1 mm at the single-cell level.
Kriti Sharma, Márton Palatinszky ... Elizabeth A Shank
Two optically transparent substrates enable the exploration of the ecophysiology and spatiotemporal organization and activities of bacteria and fungi within heterogeneous soil-like environments.
Johannes Thomsen, Magnus Berg Sletfjerding ... Nikos S Hatzakis
An open-source user-friendly toolbox implementing machine learning for single-molecule FRET analysis enabling experts and non-experts to reproducibly provide dynamic structural biology insights.
Correlative imaging of the heart at multiple spatial scales has the potential to revolutionize the way we understand deficiencies in congenital heart disease.