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Page 44 of 83
    1. Immunology and Inflammation

    Studying the biology of cytotoxic T lymphocytes in vivo with a fluorescent granzyme B-mTFP knock-in mouse

    Praneeth Chitirala, Hsin-Fang Chang ... Jens Rettig
    A new knock-in mouse endogenously labels cytotoxic granules and can be used with all modern super-resolution imaging techniques to study T cell function in vitro and in vivo.
    1. Cancer Biology
    2. Cell Biology

    Tracking cells in epithelial acini by light sheet microscopy reveals proximity effects in breast cancer initiation

    Ashna Alladin, Lucas Chaible ... Martin Jechlinger
    The presented primary epithelial acinus-based platform for studying interactions between normal and malignant cells will enable in depth analysis of the tumor initiation process on a molecular level.
    1. Neuroscience

    Sensory restoration by epidural stimulation of the lateral spinal cord in upper-limb amputees

    Santosh Chandrasekaran, Ameya C Nanivadekar ... Lee E Fisher
    Cervical spinal cord stimulation evokes sensory percepts in the missing hand and arm of people with upper-limb amputation, regardless of amputation level or time post-amputation.
    1. Computational and Systems Biology
    2. Neuroscience

    A taxonomy of seizure dynamotypes

    Maria Luisa Saggio, Dakota Crisp ... William C Stacey
    Taxonomy of seizure dynamics (TSD) provides a rigorous method for classifying and quantifying seizures and a principled framework for understanding seizure initiation and propagation.
    1. Immunology and Inflammation
    2. Neuroscience

    Differential accumulation of storage bodies with aging defines discrete subsets of microglia in the healthy brain

    Jeremy Carlos Burns, Bunny Cotleur ... Michael Mingueneau
    Two novel subsets of microglia identified by their unique autofluorescence profiles differ in their subcellular organization, proteomic signatures and in their response to aging and lysosomal dysfunction.
    1. Cell Biology

    Assigning mitochondrial localization of dual localized proteins using a yeast Bi-Genomic Mitochondrial-Split-GFP

    Gaétan Bader, Ludovic Enkler ... Hubert Dominique Becker
    The Bi-Genomic Mitochondrial-Split-GFP, where both fragments of the Split-GFP are expressed by separated translation machineries, shuts off cytosolic fluorescence of dual-localized proteins, allowing visualization of their mitochondrial echoforms.
    1. Microbiology and Infectious Disease

    Droplet-based high-throughput cultivation for accurate screening of antibiotic resistant gut microbes

    William J Watterson, Melikhan Tanyeri ... Savaş Tay
    High-throughput droplet-based cultivation of gut microbes reduces biases of traditional cultivation strategies and thereby enables detection of difficult-to-culture organisms, which is required in applications such as antibiotic screening.
    1. Neuroscience

    A Matlab-based toolbox for characterizing behavior of rodents engaged in string-pulling

    Samsoon Inayat, Surjeet Singh ... Majid H Mohajerani
    Analysis methods for video data of rodents engaged in string-pulling behavior have been developed and used to highlight differences in motion profiles of Swiss Webster and C57Bl/6 mice.
    1. Cell Biology

    Nanobody-directed targeting of optogenetic tools to study signaling in the primary cilium

    Jan N Hansen, Fabian Kaiser ... Dagmar Wachten
    Trafficking of optogenetic tools to primary cilia using nanobodies allows to study cAMP signaling with spatial and temporal resolution independent of the cell body.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Nucleosome-CHD4 chromatin remodeler structure maps human disease mutations

    Lucas Farnung, Moritz Ochmann, Patrick Cramer
    The structure of the chromatin remodeller CHD4 bound to a nucleosome reveals differences to the known Chd1-nucleosome complex and maps cancer mutations.