A systematic genetic analysis comprising seven genome-wide screens in haploid human cells uncovered new regulatory mechanisms at most levels in the WNT signaling pathway.

Heparan Sulfate Proteoglycans function as receptors for the R-spondins, a family of growth factors that amplify the strength of WNT signaling during development and in adult stem cells.

R-spondins 2 and 3 can potentiate WNT signaling in the absence of LGRs through interactions with ZNRF3/RNF43 E3 ubiquitin ligases and heparan sulfate proteoglycans, defining two alternative modes of R-spondin-mediated signaling.

New complex traits can evolve through the elimination of genes in multiple unrelated pathways, rather than through the creation of new gene functions.

AMDHD2 restricts the activity of the essential hexosamine pathway in mouse embryonic stem cells where GFPT2/GFAT2 instead of the common paralog GFPT1/GFAT1 controls metabolite entry.

Mating phenotypes are diverse in Schizosaccharomyces pombe and the natural variation can impact the spread of wtf meiotic drivers.

The dsDNA-dependent motor protein Rdh54 antagonizes Rad51-Rad54-mediated D-loop formation during homologous recombination and restricts the length of D-loops.

An unbiased genetic screen in human cells shows that the molecular target of a natural product with promising anticancer activity is a membrane phospholipid.

Dosage-compensated gene expression facilitates chromosomal aneuploidy, which presents a rapid route to phenotypic evolution in natural yeast isolates.

Protein kinase A (PKA) phosphorylates Raptor and inhibits mammalian target of rapamycin complex 1 (mTORC1) activity.