Electron cryo-tomography reveals a huge conformational change in the secretin domain of the type IV pilus machinery that occurs when the channel opens for pilus extrusion.
Giuseppina Porciello, Alessandro Monti ... Salvatore Maria Aglioti
Direct measures of inner gastrointestinal signals via ingestible capsules highlight the role of stomach pH in shaping human feelings of disgust, fear and happiness.
Andrea Serra-Marques, Maud Martin ... Anna Akhmanova
The function and distribution of kinesin motors on exocytotic vesicles is dissected and visualized through a combination of gene knockout experiments, high-resolution microscopy and advanced data analysis.
Caitlin N Spaulding, Henry Louis Schreiber IV ... Edward H Egelman
The helical rod structure and dynamic spring-like properties of the type 1 pilus are evolutionarily fine-tuned for functioning in host-pathogen interactions during urinary tract infection and gut colonization.
MT plus-end-mediated recruitment of a cortical pool of ECT2 trigger RhoA activation upon contact, which results in localized contractility during cytokinesis.
The pilus extrusion/DNA uptake system of Thermus thermophilus contains a 13-mer of the 757-residue PilQ protein and a tightly bound protein outside the outer membrane with a role in DNA binding.
Transcriptomic analysis using a novel information-based network entropy approach provides mechanistic insights into the ability of Klotho to successfully ameliorate age-related sarcopenia in old mice, but not oldest-old mice.
Samuel J Gonzalez, Julia M Heckel ... Melissa K Gardner
Computational modeling combined with biophysical and cellular experiments reveal that rapid binding to incomplete, partial microtubule binding sites facilitates tracking of the end-binding protein EB1 with growing microtubule plus-ends.