6,826 results found
    1. Cancer Biology
    2. Genetics and Genomics

    Novel insights into breast cancer copy number genetic heterogeneity revealed by single-cell genome sequencing

    Timour Baslan, Jude Kendall ... James Hicks
    Copy number alteration heterogeneity exists in many shapes and forms in breast cancer genomes and single-cell genomics is a powerful tool to further our understanding of its nature and significance.
    1. Microbiology and Infectious Disease

    A LAMP sequencing approach for high-throughput co-detection of SARS-CoV-2 and influenza virus in human saliva

    Robert Warneford-Thomson, Parisha P Shah ... Roberto Bonasio
    COV-ID is a highly scalable technology that utilizes a two-step barcoding strategy to detect viral RNAs in saliva samples combining RT-LAMP, PCR, and next generation sequencing.
    1. Neuroscience

    Rabies virus-based barcoded neuroanatomy resolved by single-cell RNA and in situ sequencing

    Aixin Zhang, Lei Jin ... Xiaoyin Chen
    Barcoded rabies virus tracing combined with single-cell RNAseq and in situ sequencing achieves high-throughput retrograde projection mapping and synaptic connectivity mapping at cellular resolution.
    1. Ecology
    2. Microbiology and Infectious Disease

    Freshwater monitoring by nanopore sequencing

    Lara Urban, Andre Holzer ... Maximilian R Stammnitz
    From now on, nanopore metagenomics can be used to monitor aquatic environments with high spatiotemporal resolution, by use of a benchmarked, standardised and cost-effective workflow.
    1. Microbiology and Infectious Disease

    Targeted genomic sequencing with probe capture for discovery and surveillance of coronaviruses in bats

    Kevin S Kuchinski, Kara D Loos ... Andrew DS Cameron
    Hybridization probe capture is useful for discovery and surveillance of novel coronaviruses, and its unique strengths complement existing methods like amplicon sequencing and deep metagenomic sequencing.
    1. Genetics and Genomics
    2. Microbiology and Infectious Disease

    Tiled-ClickSeq for targeted sequencing of complete coronavirus genomes with simultaneous capture of RNA recombination and minority variants

    Elizabeth Jaworski, Rose M Langsjoen ... Andrew L Routh
    Tiled-ClickSeq provides a simple and novel next-generation sequencing approach for complete genome sequencing of viruses including SARS-CoV-2, whilst capturing RNA recombination events and minority variants.
    1. Computational and Systems Biology

    Gene regulatory network reconstruction using single-cell RNA sequencing of barcoded genotypes in diverse environments

    Christopher A Jackson, Dayanne M Castro ... David Gresham
    Single cell expression data can be used to determine how regulatory transcription factors and target genes are connected, and is especially useful when studying transcription factors controlling heterogeneous cell states.
    1. Computational and Systems Biology
    2. Genetics and Genomics

    Genome-wide detection of imprinted differentially methylated regions using nanopore sequencing

    Vahid Akbari, Jean-Michel Garant ... Steven JM Jones
    A genome-wide map of human allele-specific methylation using long-read sequencing detects novel imprinted DNA methylation events and reveals large blocks of subtle parent-of-origin bias in DNA methylation with mutual exclusive allelic H3K36me3 and H3K27me3 histone modifications.
    1. Epidemiology and Global Health

    Previously undetected super-spreading of Mycobacterium tuberculosis revealed by deep sequencing

    Robyn S Lee, Jean-François Proulx ... William P Hanage
    Incorporating within-host diversity in transmission, as identified by deep sequencing, can significantly change previously-held inferences, with major implications for genomic studies of transmission in tuberculosis and other pathogens as well.
    1. Developmental Biology
    2. Stem Cells and Regenerative Medicine

    Single-cell RNA sequencing reveals cellular and molecular heterogeneity in fibrocartilaginous enthesis formation

    Tao Zhang, Liyang Wan ... Hongbin Lu
    Single-cell RNA sequencing to delineate the comprehensive postnatal RC enthesis growth from as early as postnatal day 1 to postnatal day 56.

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