108 results found
    1. Cell Biology

    NECAPs are negative regulators of the AP2 clathrin adaptor complex

    Gwendolyn M Beacham, Edward A Partlow ... Gunther Hollopeter
    Endocytosis is regulated by a protein family that recycles the AP2 clathrin adaptor by restoring the inactive form of complex.
    1. Cell Biology
    2. Structural Biology and Molecular Biophysics

    A structural mechanism for phosphorylation-dependent inactivation of the AP2 complex

    Edward A Partlow, Richard W Baker ... Gunther Hollopeter
    Cryo-EM reveals how a protein called NECAP inactivates the AP2 clathrin adaptor complex through concerted engagement of two domains which confer specificity for membrane-activated and phosphorylated AP2.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Ligand modulation of sidechain dynamics in a wild-type human GPCR

    Lindsay D Clark, Igor Dikiy ... Daniel M Rosenbaum
    Generation of a highly deuterated 13C-methyl labeled wild-type GPCR sample is used to facilitate characterization of the molecular environments and fast ps-ns dynamics of sidechains when the receptor is bound to ligands of different efficacy.

  4. Open Peer Reviewers in Africa: A ‘train-the-trainer’ program pilot recap

    As the pilot of the Open Peer Reviewers in Africa project comes to an end, AfricArXiv, Eider Africa, eLife, PREreview, and the Training Centre in Communication Africa reflect on this collaborative effort to promote best open peer-review practices in Africa.
    1. Neuroscience

    A nap to recap or how reward regulates hippocampal-prefrontal memory networks during daytime sleep in humans

    Kinga Igloi, Giulia Gaggioni ... Sophie Schwartz
    Sleep plays a critical role in the selectivity of memory by promoting the retention of important memories at the expense of less relevant ones.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Cryo-EM structure of the adenosine A2A receptor coupled to an engineered heterotrimeric G protein

    Javier García-Nafría, Yang Lee ... Christopher G Tate
    The adenosine A2a receptor couples to the heterotrimeric G protein Gs using both conserved contacts seen in other complexes and, in addition, novel contacts to the beta subunit of the G protein.
    1. Immunology and Inflammation
    2. Microbiology and Infectious Disease

    Rapid and sensitive detection of SARS-CoV-2 infection using quantitative peptide enrichment LC-MS analysis

    Andreas Hober, Khue Hua Tran-Minh ... Fredrik Edfors
    Immuno-affinity enrichment combined with liquid chromatography-mass spectrometry can be used to detect viral proteins to confirm the presence of SARS-CoV-2 in clinical samples.
    1. Structural Biology and Molecular Biophysics
    2. Plant Biology

    Kinetics of the LOV domain of ZEITLUPE determine its circadian function in Arabidopsis

    Ashutosh Pudasaini, Jae Sung Shim ... Brian D Zoltowski
    In the plant circadian clock photoreceptor ZEITLUPE, evolutionarily selected residues distinguish photocycle kinetics and allosteric signal transduction pathways to permit proper circadian timing.
    1. Structural Biology and Molecular Biophysics

    Allosteric modulation of the adenosine A2A receptor by cholesterol

    Shuya Kate Huang, Omar Almurad ... R Scott Prosser
    Cholesterol exhibits transient interaction with A2AR and regulates receptor conformational equilibria through indirect membrane effects.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    A clathrin coat assembly role for the muniscin protein central linker revealed by TALEN-mediated gene editing

    Perunthottathu K Umasankar, Li Ma ... Linton M Traub
    The muniscin protein FCHO1 interacts with, and activates, the adapter protein-2 complex (AP-2) to promote the assembly of clathrin-coated vesicles.
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