Electron tomography and biochemical approaches demonstrate a direct role for WDR35, beyond integrity of the IFT-A holocomplex, in the formation and fusion of electron-dense-coated vesicles to the ciliary sheath and pocket for delivery of cargos necessary for axoneme elongation.

The speed of intraflagellar transport, influenced by particle size, shows a positive correlation with ciliary length in zebrafish.

Six BBS proteins form a core BBSome transport vehicle, which is sufficient for recognizing membrane proteins for transport into the ciliary compartment.

During intraflagellar transport (IFT), kinesin-II dissociates from IFT trains at the flagellar tip and its diffusion in flagella serves as a negative feedback mechanism that facilitates flagellar length control in Chlamydomonas.

Cholesterol accessibility in the plasma membrane can be controlled by proteins and function as a signaling messenger in cell-cell communication pathways.

The combination of data and modelling shows the mechanisms underlying bidirectional flow of cerebrospinal fluid and its impact on long range transport in the CSF between brain and spinal cord.

Bardet–Biedl syndrome 3 (BBS3) protein promotes phospholipase D to load onto the BBSome at the ciliary tip for moving out of the cilium via intraflagellar transport.

The cargo adapter ARMC2 mediates the association of radial spoke complexes to IFT trains ensuring radial spoke import and assembly in Chlamydomonas flagella.

During cilium-generated signaling, ciliary membrane protein trafficking is unidirectional and ciliary membrane protein composition is regulated through action in the cytoplasm of the retrograde intraflagelllar transport (IFT) motor and shedding of ciliary ectosomes.

The patched hedgehog receptor inhibits the transmembrane transducer smoothened by reducing the accessibility of cholesterol locally at the membrane of the primary cilium.