The automation of cryo-focused ion beam milling enables previously unfeasible cryo-electron tomography projects.

The PIE-scope resolves the limitations in correlative cryo-focused ion beam milling by adding robustness and ease of use to the workflow.

Implementation and characterisation of plasma-focused ion beams for volumetric imaging of cryogenically preserved cells and tissues for ultrastructural examination in their native state.

The first 3D views of the native algal chloroplast provide new insights into thylakoid biogenesis, photosynthesis, and carbon fixation.

SerialFIB is an adaptable open-source software designed for streamlining, advanced development and automation of cryo-focused ion beam-based cellular preparations for in situ cryo-electron tomography.

Integrating a cryogenic light microscope with a focused ion beam/scanning electron microscope, allows directly targeting of fluorescent structure when preparing a frozen-hydrated lamella, without the need for repositioning or fiducial markers.

Complete neuronal circuits can be reconstructed, and whole eukaryotic cells imaged, at less than 10 nm isotropic resolution.

In situ cryo-electron tomography unveils the molecular sociology of a developing sporangium in Bacillus subtilis, revealing critical information about cell wall remodeling and membrane migration in bacteria.

Mature myelin sheaths are scaffolded by the anillin-dependent assembly of septin filaments, thereby facilitating rapid nerve conduction in the healthy CNS.

Correlative microscopy and electron cryo-tomography on apoptotic HeLa cells reveal remodeling of outer and inner mitochondrial membranes, Bax cluster ultrastructure and ATP synthase reorganization in ruptured mitochondria.