Browse our latest Biochemistry and Chemical Biology articles

Human induced pluripotent stem cells and embryonic stem cells express a similar set of proteins, but the levels of cytoplasmic and mitochondrial proteins vary, with concomitant effects on phenotypes including transport and mitochondrial function.

A novel pair of isobaric crosslinking reagents is described, which allow relative quantification of crosslinker-modified peptides during mass spectrometry analysis for comparative structural studies of proteins and protein complexes.

An approachable framework for the scalable implementation of proteome-wide thermal shift assays to assess drug mechanisms of action.

SLC35G1 is the first highly chloride-sensitive transporter localized on the basolateral membrane of intestinal epithelial cells, responsible for citrate absorption.

Robust NMR approach allowed simultaneous detection of substrate cleavage site and real-time kinetics of hydrolysis for Staphylococcus aureus peptidoglycan hydrolases lysostaphin and LytM, and revealed D-alanyl-glycine activity for LytM.

For mRNA decapping by enhancing DCP2's mRNA-binding affinity and regulating distinct biological processes through DCP1a and DCP1b, human DCP1 is essential.

Improved technology for counting proteins captures myosin 10 dynamics throughout the filopodial lifecycle, defines requirements for initiating filopodia, and reveals a crowded filopodial tip where motors frequently exceed available actin.