Secondary metabolites rocaglates produced by plants lead to a plant‒fungus tug-of-war, utilizing unique amino acid substitutions in eIF4A, a target of the compounds.
Kevin Chun-Ho Ngan, Samuel M Hoenig ... Brian B Liau
Activity-based inhibitors, in conjunction with new experimental and computational tools, enable the discovery of mechanisms involved in DNMT1 autoinhibition.
Marco Caligaris, Raffaele Nicastro ... Claudio De Virgilio
Discovery of new Snf1/AMPK targets in the TORC1 pathway highlights the complex, multilayered crosstalk between major signaling pathways in the regulation of nutrient deprivation sensing.
The development of an integrative computational-experimental approach for identifying small molecules that can disrupt RNA:protein interactions in vitro and in cells led to the identification of several inhibitors of YBX1 in the micromolar range, including a previously approved drug.
Spatial transcriptomics analysis of rotavirus replication factories reveals principles of viral transcript partitioning and RNA stoichiometry in these granules.
Hidehiko Okuma, Jeffrey M Hord ... Kevin P Campbell
N-terminal domain of dystroglycan enables like-acetylglucosaminyl transferase to elongate matriglycan on α-dystroglycan and prevent skeletal muscle pathophysiology.
A high-throughput analysis of several self-cleaving ribozymes reveals the effect of every possible mutation, and every possible pair of mutations, and how patterns in the relative activity data can be mapped to canonical and non-canonical structural elements.
Abhinay Ramaprasad, Paul-Christian Burda ... Michael J Blackman
The malaria parasite uses an enzyme called GDPD to absorb choline, an important nutrient, from the bloodstream and this is essential for the parasite to survive inside the red blood cell.
Cryo-EM structure and functional analyses show that human iron exporter ferroportin (Fpn) can bind and transport calcium ions and the transport activity is reduced when the concentration of ferrous ions is high.
