Browse our latest Biochemistry and Chemical Biology articles

Structural and biochemical analysis of the mechanism of paroxetine binding to the serotonin transporter provides a framework for transporter inhibition and design of small-molecule inhibitors.

High-throughput phenotypic screening followed by unbiased target identification reveals a new molecular glue HQ461 that induces CDK12-DDB1 interaction to promote degradation of Cyclin K via the ubiquitin proteasome system.

Simple guidelines and a checklist are provided for performing high-quality equilibrium binding measurements.

When translation stops, cells require intracellular acidification to turn on the conserved heat shock response during stress, and stress-triggered acidification (common in eukaryotes) is adaptive, promoting cell and population fitness.

Hydrogen-deuterium exchange, electron microscopy, and vesicle reconstitution show how binding of the autophagy adaptor NDP52 to the FIP200 subunit of the ULK1 complex triggers membrane binding in autophagy.

A new cellular transport mechanism splits the substrate and separately translocates its fragments to achieve selectivity.

The first atomic resolution structure of a mitochondrial respiratory complex from plants provides insight into the assembly and evolution of respiration in autotrophic eukaryotes.

In order to enter a cell, an ammonium ion must first dissociate to form an ammonia molecule and a hydrogen ion (a proton), which then pass through the cell membrane separately and recombine inside.

Cryo-EM structures of human MCU-EMRE-MICU1-MICU2 complex in the apo and Ca²⁺-bound states reveal how MICU1 and MICU2 impart their gating function to the mitochondrial calcium uniporter.

Protein stability in the cage formed by the chaperonin GroEL and its cofactor GroES is reduced by more than 5 kcal mol^-1 relative to that in bulk solution.