Domenica Ippolito, Saurabh Thapliyal, Dominique A Glauser
Ca2+-dependent nuclear entry of Ca2+/calmodulin-dependent protein kinase-1 in sensory neurons, taking place with a relatively slow kinetics, contributes to couple long-lasting sensory stimulations with signaling in the nucleus over a timescale relevant for sensory history-dependent plasticity.
Ligand-specific-induced conformations of M3 muscarinic receptors reveal similar requirements for G protein-coupled receptor kinase 2 and Gq protein binding, whereas arrestin3 binding is mediated by distinct receptor conformations.
Maria Podinovskaia, Cristina Prescianotto-Baschong ... Anne Spang
A novel, inexpensive, easy-to-use method to analyse traffic along the endosomal pathway in mammalian cells shows that there is coordination between Rab conversion and acidification, but almost none between Rab conversion and recycling.
SNARE–SNARE interactions that have previously only been proposed to participate in membrane fusion on the nanoscale also serve to organize SNARE domains on the mesoscale.
A proximity labeling approach defines germ granule proteome in Caenorhabditis elegans, and identifies LOTUS domain proteins as key regulators of germ granule assembly.
Shannon Rausser, Caroline Trumpff ... Martin Picard
A high-throughput mitochondrial phenotyping approach quantifies mitochondrial features among purified human immune cell subtypes and provides foundational knowledge to map inter- and intra-individual variation in mitochondrial energetics with high biological specificity.
Condensation of cytosolic protein YBX1 into processing bodies reveals mechanisms for efficient and selective sorting of proteins and their cognate microRNAs into exosomes.
Analysis of insulin secretion patterns in pancreatic islets shows that microtubules control β-cell heterogeneity by regulating initiation and timing of secretion hot spot activity in parallel with Ca2+ signaling.
Redaction, intended to obscure personal and institutional identity, reduced the gap between Black vs. White applicants in simulated review of real NIH scientific grant applications, thus providing qualified support for double-blinded models of peer review.
