Neural stem cells dynamically polarize through the stepwise activity of polarized cortical targeting and cortical flows that coalescence discontinuous cortical patches into an organized cap structure.
An unbiased genetic screen in Drosophila provides evidence for a direct link between glial Ca2+ 25 signaling and classical functions of glia in buffering external K+ as a mechanism to regulate neuronal excitability.
Loïc Binan, François Bélanger ... Santiago Costantino
Single cells from a large heterogeneous population can be identified, isolated and clonally expanded using commonly available microscopy equipment and simple reagents, based solely on visual characteristics.
Neurotrophin deprivation leads to rapid caspase-independent disassembly of the actin-spectrin-based membrane skeleton and spectrin-dependent retrograde signaling in axon degeneration.
In a novel cell-in-cell interaction termed emperipolesis, neutrophils routinely transit through the cytoplasm of bone marrow megakaryocytes to pass surface membrane to newly-generated hybrid platelets.
Both exogenous and endogenous genes can be highly activated in cells by a new dCas9-based activator, CRISPR-assisted trans enhancer, that can be used to activate genes for biomedical applications.
Restoration of molecular and morphological symmetry and mechanical integrity following epithelial fusion relies on adaptive changes that are mediated by cytoskeletal tension and Bazooka dependent modulation of fusing interface geometry.
Simeon Leupold, Georg Hubmann ... Matthias Heinemann
With replicative age, S. cerevisiae shifts from a fermentative towards an unusual respiratory metabolism, accompanied by a decrease in growth and glucose uptake rate.
