During intraflagellar transport (IFT), kinesin-II dissociates from IFT trains at the flagellar tip and its diffusion in flagella serves as a negative feedback mechanism that facilitates flagellar length control in Chlamydomonas.
Valentina Galli, Rafael Sebastian ... Aurélien Roux
Uncoupling of dynamin polymerization and GTPase activity can be induced with dynamin, which allows in vivo visualization of its activity at endocytic pits.
Loading of CD95 and CD95L-derived sequences into the RNA-induced silencing complex elicits a distinct form of RNAi-mediated cell death of cancer cells that results from the targeting of multiple survival genes.
Deletion of delta-tubulin or epsilon-tubulin in human cells results in loss of centriolar triplet microtubules and defects in centriole structure and inheritance.
Nicholas R Guydosh, Philipp Kimmig ... Rachel Green
A critical component of the cellular response to unfolded proteins is the widespread rescue of ribosomes that stall on endonucleolytically-cleaved mRNA transcripts.
A detailed analysis of protein abundance and phosphorylation changes across mitotic subphases and interphase in asynchronously growing human cells has been enabled by combining FACS with quantitative MS-based proteomics.
YTHDC1 facilitates selective clearance of N6-methyladenosine methylated mRNAs from the nucleus to the cytoplasm through binding by nuclear 'reader' proteins and incorporation into the canonical mRNA export pathway.
