A comprehensive toolkit enables genome-wide, recombinase-independent mosaic analysis in Drosophila, permitting clonal analysis of pericentromeric genes, deficiency chromosomes, and interspecific hybrids previously inaccessible to standard methods.
Mammalian oocytes utilize a conserved combination of cell cycle control, cytoskeletal organization, and polarity pathways that are fundamentally shared with other vertebrates and invertebrates.
Prateek D Bansal, Maia Kinnebrew ... Diwakar Shukla
Multiple competing hypotheses concerning the cholesterol accessibility of oncoprotein Smoothened are quantitatively assessed and experimentally validated, showing that cholesterol takes two pathways from either membrane leaflet to the binding sites.
Single-cell transcriptomics data from mouse bone-relevant cells was used to inform human bone mineral density genome-wide association studies and prioritize genetic targets with potential causal roles in the development of osteoporosis.
Roberta Carloni, Tadhg Devlin ... Robin C Allshire
Synthetic proteins designed to bind specific repetitive DNA sequences in the Trypanosoma brucei genome allow the identification of proteins enriched over different DNA elements.
Proteome-scale visual screening in budding yeast provides a functional catalog of plasma membrane repair proteins and reveals the coordinated cellular responses at the bud tip and the damage site.
Comprehensive mapping of wing sensory neurons in Drosophila reveals that some proprioceptors make direct connections onto flight steering motor neurons, enabling rapid feedback control during flight.
