1. Immunology and Inflammation
  2. Microbiology and Infectious Disease

SARS-CoV-2 host-shutoff impacts innate NK cell functions, but antibody-dependent NK activity is strongly activated through non-spike antibodies

  1. Ceri Alan Fielding
  2. Pragati Sabberwal
  3. James C Williamson
  4. Edward JD Greenwood
  5. Thomas WM Crozier
  6. Wioleta Zelek
  7. Jeffrey Seow
  8. Carl Graham
  9. Isabella Huettner
  10. Jonathan D Edgeworth
  11. David A Price
  12. Paul B Morgan
  13. Kristin Ladell
  14. Matthias Eberl
  15. Ian R Humphreys
  16. Blair Merrick
  17. Katie Doores
  18. Sam J Wilson
  19. Paul J Lehner
  20. Eddie CY Wang
  21. Richard J Stanton  Is a corresponding author
  1. Division of Infection and Immunity, School of Medicine, Cardiff University, United Kingdom
  2. Cambridge Institute for Therapeutic Immunology & Infectious Disease, Jeffrey Cheah Biomedical Centre, Cambridge Biomedical Campus, University of Cambridge, United Kingdom
  3. Department of Infectious Diseases, School of Immunology & Microbial Sciences, King's College London, United Kingdom
  4. Department of Infectious Diseases, Guy’s and St Thomas’ NHS Foundation Trust, United Kingdom
  5. MRC - University of Glasgow Centre for Virus Research, United Kingdom
https://doi.org/10.7554/eLife.74489
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Cite this article
  1. Ceri Alan Fielding
  2. Pragati Sabberwal
  3. James C Williamson
  4. Edward JD Greenwood
  5. Thomas WM Crozier
  6. Wioleta Zelek
  7. Jeffrey Seow
  8. Carl Graham
  9. Isabella Huettner
  10. Jonathan D Edgeworth
  11. David A Price
  12. Paul B Morgan
  13. Kristin Ladell
  14. Matthias Eberl
  15. Ian R Humphreys
  16. Blair Merrick
  17. Katie Doores
  18. Sam J Wilson
  19. Paul J Lehner
  20. Eddie CY Wang
  21. Richard J Stanton
(2022)
SARS-CoV-2 host-shutoff impacts innate NK cell functions, but antibody-dependent NK activity is strongly activated through non-spike antibodies
eLife 11:e74489.
https://doi.org/10.7554/eLife.74489
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Abstract

The outcome of infection is dependent on the ability of viruses to manipulate the infected cell to evade immunity, and the ability of the immune response to overcome this evasion. Understanding this process is key to understanding pathogenesis, genetic risk factors, and both natural and vaccine-induced immunity. SARS-CoV-2 antagonises the innate interferon response, but whether it manipulates innate cellular immunity is unclear. An unbiased proteomic analysis determined how cell surface protein expression is altered on SARS-CoV-2-infected lung epithelial cells, showing downregulation of activating NK ligands B7-H6, MICA, ULBP2, and Nectin1, with minimal effects on MHC-I. This occurred at the level of protein synthesis, could be mediated by Nsp1 and Nsp14, and correlated with a reduction in NK cell activation. This identifies a novel mechanism by which SARS-CoV-2 host-shutoff antagonises innate immunity. Later in the disease process, strong antibody-dependent NK cell activation (ADNKA) developed. These responses were sustained for at least 6 months in most patients, and led to high levels of pro-inflammatory cytokine production. Depletion of spike-specific antibodies confirmed their dominant role in neutralisation, but these antibodies played only a minor role in ADNKA compared to antibodies to other proteins, including ORF3a, Membrane, and Nucleocapsid. In contrast, ADNKA induced following vaccination was focussed solely on spike, was weaker than ADNKA following natural infection, and was not boosted by the second dose. These insights have important implications for understanding disease progression, vaccine efficacy, and vaccine design.

Editor's evaluation

By using a systematic proteomics approach, the authors demonstrated that SARS-CoV2 remodels the plasma membrane of infected human epithelial cells. Although the study indicates the manipulation of different immune response pathways, it seems that in the focus of viral immunoevasion are natural killer (NK) cells, which play a crucial role in controlling early viral infection. However, antibody-dependent NK cell activation was observed later in the disease process. These findings could have implications for the understanding of SARS-CoV-2 control by the immune system and vaccine development.

https://doi.org/10.7554/eLife.74489.sa0

Introduction

COVID-19 studies have focused on interferon responses during the early innate phase of infection, and neutralising antibodies and virus-specific T-cells during the adaptive phase. In contrast, and despite their importance in antiviral protection (Pierce et al., 2020), considerably less is known about the role of Natural Killer (NK) cells in infection. NK cells bridge the innate and adaptive responses, and individuals with NK cell deficiencies suffer severe viral infections (Orange, 2013). NK cells respond rapidly to viruses, directly killing infected cells by releasing cytotoxic granules. They promote inflammation through the release of TNFα and IFNγ, and influence the induction of both B- and T-cell responses. Once virus-specific antibodies are induced, antibody-dependent NK activation (ADNKA) is generated through antibody linking cell-surface viral antigens with NK cell Fc receptors and subsequent antibody-dependent cellular cytotoxicity (ADCC).

The majority of studies on NK cells have focussed on their adaptive roles in established COVID-19 infection, with highly activated NK cells seen in both the periphery and the lung (Maucourant et al., 2020). The genetic loss of NKG2C (a hallmark of adaptive NK cells, capable of enhanced ADCC) is also a risk factor for severe disease (Vietzen et al., 2021b), implying a protective role in COVID-19 infection. In support of this, in animal models, Fc-mediated functions correlate with protection in vaccination (Gorman et al., 2021), and monoclonal antibody-mediated control of SARS-CoV-2 infection is enhanced by Fc-dependent mechanisms (Chan et al., 2020; Schäfer et al., 2021; Winkler et al., 2020; Suryadevara et al., 2021; Winkler et al., 2021; Yamin et al., 2021), which enable control of disease even in the absence of neutralising activity (Beaudoin-Bussières et al., 2021). Furthermore, although neutralising monoclonal antibodies are effective when administered prophylactically, Fc-activity is required for efficacy when administered to animals therapeutically (Yamin et al., 2021).

These animal studies demonstrate potentially important roles for adaptive NK cell responses in COVID-19. Despite this, our understanding of ADCC following infection and vaccination in humans remains limited. Studies to date have assumed spike protein is the dominant ADCC target, and have tested plate-bound protein or transfected cells (Barrett et al., 2021; Herman et al., 2021; Zohar et al., 2020; Tauzin et al., 2021; Anand et al., 2021; Lee et al., 2020; Dufloo et al., 2020; Tortorici et al., 2020; McCallum et al., 2021; Cathcart et al., 2021). Using these systems, they have shown that ADNKA-inducing antibodies are generated following SARS-CoV-2 infection (Herman et al., 2021; Anand et al., 2021; Lee et al., 2020; Dufloo et al., 2020), are induced by vaccination in humans (Barrett et al., 2021; Tauzin et al., 2021), and can be mediated by a subset of neutralising monoclonal antibodies targeting the spike protein (Tortorici et al., 2020; McCallum et al., 2021; Cathcart et al., 2021). However, these responses have not been tested against SARS-CoV-2 infected cells; the presence, abundance, accessibility, and conformation of viral glycoproteins can be very different during productive infection. Furthermore, although viral entry glycoproteins such as spike can be found on the infected cell surface, and can mediate ADCC, other viral proteins can be the dominant mediators of ADCC during infection; indeed, we have shown that in other viruses, non-structural proteins are often the dominant targets (Vlahava et al., 2021).

In addition, there is little information on the NK cell response to SARS-CoV-2 infected cells during the innate phase of infection, despite this process being important to virus pathogenesis; successful viruses must counteract robust NK activation to establish infection, and their ability to do this is critical to their ability to cause disease (Patel et al., 2018; Berry et al., 2020). Whether SARS-CoV-2’s ability to infect humans is dependent on viral evasion of innate cellular immunity, is unknown. NK cell activation is complex and depends on the balance of ligands for a wide range of inhibitory or activating receptors (Kumar, 2018). These ligands can be induced on the surface of target cells in response to stress, infection, or transformation. For example, the stress ligands MICA, MICB, and ULBP1-6, all bind to the ubiquitously expressed activating receptor NKG2D. To limit NK cell activation, many viruses manipulate these NK activating ligands, reducing NK-cell-mediated control (Patel et al., 2018). Whether similar manipulations underlie the ability of SARS-CoV-2 to cause disease in humans remains unclear.

To address these critical gaps in our understanding of the SARS-CoV-2-specific NK cell response, we used quantitative proteomics to determine how SARS-CoV-2 infection affected cell surface protein expression (Rihn et al., 2021). We found that SARS-CoV-2-infected cells downregulate multiple activating NK ligands, resulting in a reduced NK cell response to infected cells. This suggests a novel mechanism by which SARS-CoV-2 counteracts innate immunity early in infection, to establish disease. The development of humoral immunity following natural infection resulted in robust ADNKA against infected cells, and high levels of proinflammatory cytokines; these responses persisted for at least 6 months in most patients. Proteomics identified four cell-surface viral proteins capable of driving this response, leading to the surprising discovery that ADNKA is not primarily driven by spike antibody, but was dominated by antibody to other viral proteins such as nucleocapsid, membrane and ORF3a; this defines a novel role for antibodies targeting the immunodominant nucleocapsid protein. In agreement with these findings, and in contrast to plate-bound protein or transfected cells, spike was a poor activator of ADNKA following vaccination when tested using infected cells. This implies that the breadth and magnitude of ADNKA responses is limited in current vaccination strategies. The inclusion of antigens such as nucleocapsid would recruit additional effector mechanisms, and may help maintain vaccine efficacy following mutation of spike in naturally circulating virus variants.

Results

SARS-CoV-2 remodels the plasma membrane proteome

Immune cells recognise and interact with infected cells through cell surface ligands. We therefore wanted to gain a comprehensive and unbiased overview of how SARS-CoV-2 infection changes the plasma membrane protein landscape. We performed plasma membrane enrichment through selective aminooxy-biotinylation (Plasma Membrane Profiling; PMP), a methodology that we previously developed (Weekes et al., 2010; Weekes et al., 2014) and have validated extensively against human immunodeficiency virus (HIV) (Matheson et al., 2015), human cytomegalovirus (HCMV) (Vlahava et al., 2021; Weekes et al., 2014; Fielding et al., 2017; Hsu et al., 2015; Weekes et al., 2013), and herpes simplexvirus (HSV) (Soh et al., 2020) infection.

High infection rates are critical to minimise confounding effects from bystander cells. We therefore applied PMP to SARS-CoV-2 infected A549-ACE2-TMPRSS2 (AAT) cells, which are highly permissive to SARS-CoV-2 (Rihn et al., 2021) and infection at a high multiplicity of infection (MOI) results in over 90% infection by 24 hr (Figure 1A). We utilised TMT-based quantification to compare plasma membrane (PM) protein abundance in uninfected cells, and cells infected for 12, 24, and 48 hr (Figure 1B). In total, 4953 proteins were quantitated, including 2227 proteins with gene ontology annotation associated with the cell surface and plasma membrane (Figure 1—figure supplement 1A). Importantly however, the plasma membrane annotated proteins made up nearly 80% of the total protein abundance, indicating a high degree of enrichment for plasma membrane proteins, comparable with our previous PMP datasets (Vlahava et al., 2021; Weekes et al., 2010; Weekes et al., 2014; Matheson et al., 2015; Fielding et al., 2017; Hsu et al., 2015; Weekes et al., 2013; Soh et al., 2020). The detection of low abundance proteins that are not plasma-membrane annotated reflects a combination of incorrect annotations, and contaminating intracellular proteins that were incompletely removed following PMP.

Figure 1 with 3 supplements see all
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SARS-CoV-2 remodels the plasma membrane proteome.

(A) AAT cells were infected with SARS-CoV-2 in biological triplicate (MOI = 5). Twenty-four hr later, they were detached with trypsin, fixed and permeabilised, stained for Spike protein, and analysed by flow cytometry. (B) Schematic of plasma membrane profiling and analysis pathway. (C). Examples of temporal profiles of viral and cellular genes, fold change is compared to 0 hr timepoint. Data points show mean ± SD. (D). Left – heat map of the 914 significantly changing proteins clustered by k-mean, colour indicates log2 fold change compared to 0 hr, right, Z-score normalised temporal profiles of proteins within each cluster.

Five SARS-CoV-2 viral proteins were detected (see later), including spike, ORF3A, and membrane, which have previously been annotated as having a cell surface localisation (Figure 1C). Consistent with prior reports, the viral receptor ACE2 was strongly downregulated after infection (Patra et al., 2020), while TMPRSS2 expression was unaffected (Figure 1C). Overall, of the 2227 PM proteins quantified with more than one peptide, 914 showed changes which were both significant and with a greater than 1.5-fold alteration over the course of the experiment. Clustering of proteins by their temporal pattern of change has previously shown utility in defining classes of regulated proteins and predicting underlying mechanisms (Matheson et al., 2015; Greenwood et al., 2016). Here, we used K-means clustering to define five groups of significantly altered proteins by their temporal profile.

To assess the innate immune response triggered by SARS-CoV-2 infection, the clusters were interrogated for the presence of a predefined list of interferon alpha-inducible genes. Thirteen of the 23 detected PM genes associated with the interferon response fell into cluster 5 (Figure 1—figure supplement 1B, Supplementary file 2), which describes proteins with an increase in expression at 48 hr, examples shown in Figure 1—figure supplement 1C. As the SARS-CoV-2 viral replication cycle is completed in less than 24 hr (Lei et al., 2020), SARS-CoV-2 is able to evade innate immune recognition and/or suppress IFN signalling until long after virus egress has been achieved.

To identify potential innate and adaptive immune evasion strategies employed by SARS-CoV-2, we focused on cluster 1, which describes proteins downregulated from early in the time course. This cluster was subject to gene ontology and pathway analysis to define proteins related by functional and structural similarities (Figure 2). Several classifications enriched in this cluster are particularly relevant, including the downregulation of several cytokine receptors, including IFNAR1. Much of the SARS-CoV-2 genome is dedicated to antagonising a type I interferon response within an infected cell (Lei et al., 2020) and the plasma membrane downregulation of IFNAR1 presents a previously undescribed strategy to prevent signalling from exogenous IFN.

Figure 2 with 1 supplement see all
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SARS-CoV-2 manipulates multiple classes of plasma membrane proteins.

Enriched gene ontology and pathway annotations of proteins downregulated in temporal cluster 1 were condensed to groups of related terms, bar chart shows the enrichment score of these groups. Examples of annotation terms falling within each group are shown underlined with Bejamani-Hochberg adjusted p-values in parentheses, alongside four examples of proteins for each annotation.

Also of note is the downregulation of heparan sulfate proteoglycans, the syndecan (SDC), glypican (GPC), and neurophillin (NRP) proteins, which together encompass the families of heparan sulfate modified proteins expressed on epithelial cells (Sarrazin et al., 2011). NRP1 is a cell entry factor for SARS-CoV-2 (Cantuti-Castelvetri et al., 2020; Daly et al., 2020), and SARS-CoV-2 spike is also reported to bind heparan sulphated proteins (Clausen et al., 2020). The decrease in cell surface expression of heparan sulfated proteins may therefore enable the egress of viral particles and prevent super-infection, and is reminiscent of the capacity of other viruses to downregulate both primary viral receptors and co-receptors (Landi et al., 2011).

SARS-CoV-2 inhibits the synthesis of multiple NK cell ligands and reduces NK activation

Our experience with PMP has emphasised the modulation of NK ligands as an important immune evasion mechanism (Fielding et al., 2017). As these ligands are poorly annotated in public databases, we investigated the PMP dataset for previously described ligands (Kumar, 2018), of which 18 were detected (Figure 2—figure supplement 1). MHC-I proteins HLA-B to HLA-E were upregulated in cluster 5, likely reflecting a late IFN-mediated upregulation, while HLA-A was unchanged. By contrast, the activating NK-cell ligands MICA, ULBP2, B7-H6 (NCR3LG1), and Nectin1 (which is inhibitory in mice, but recently reported as activating in humans Holmes, 2019) were significantly downregulated in cluster 1. This was confirmed by flow cytometry in multiple cell types (Figure 3A, Figure 3—figure supplement 1A). These responses were dependent on virus replication, because no alterations in ligand abundance were observed when virus was heat-inactivated (Figure 3—figure supplement 2A).

Figure 3 with 2 supplements see all
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SARS-CoV-2 infection inhibits the expression of multiple NK ligands.

(A) AAT cells were either mock infected, or infected with SARS-CoV-2 for 24 hr (MOI = 5), detached using TrypLE, then stained for the indicated NK cell receptors before being analysed by flow cytometry (bottom). Plots for the same proteins from PMP are included for reference (top) (B) AAT cells were infected with RAd-MICA, RAd-ULBP2, or RAd-B7-H6, then after 48 hr either mock infected, or infected with SARS-CoV-2 (SCV2) for a further 24 hr (MOI = 5). Lysates were either kept undigested, or digested with EndoH (E), or PNGaseF (P), then western blotted for the indicated proteins. Mature cell surface glycoproteins are resistant to EndoH, while immature (ER resident) forms are digested and therefore run with a smaller mass following EndoH digestion (C) AAT cells were infected with RAd-MICA or RAd-B7-H6, then after 24 hr either mock infected, or infected with SARS-CoV-2 (SCV2), and treated with MG132 (M) or Leupeptin (L).Twenty-four hour later lysates were made, and analysed by western blot for the indicated proteins. (Note: The additional band seen when staining for actin in the presence of SARS-CoV-2 may be non-specific).

Many of the best characterised mechanisms by which viruses inhibit the function of NK ligands involve inhibition of cell-surface transport and/or proteasomal or lysosomal degradation of the mature protein (Patel et al., 2018; Berry et al., 2020). To determine whether SARS-CoV-2 targeted NK ligands in the same way we measured their abundance, and their sensitivity to digestion with EndoH, which cleaves N-linked glycans from proteins that have not yet transited (or have been retained in) the endoplasmic reticulum (ER). Digestion with PNGaseF (which cleaves N-linked glycans irrespective of subcellular trafficking) served as a control to show the effect of complete deglycosylation. We were unable to detect endogenous NK ligands by western blot (data not shown), and therefore overexpressed MICA, ULBP2, and B7-H6 using replication deficient adenovirus vectors (RAd). Following infection with SARS-CoV-2, levels of all three NK ligands were substantially reduced, however there was no alteration in the pattern of EndoH sensitivity (Figure 3B). Thus, SARS-CoV-2 infection does not result in intracellular retention of NK ligands, but instead reduces the overall abundance of protein.

To determine whether this occurred by inhibition of synthesis or degradation of mature protein, we treated cells with proteasomal (MG132) or lysosomal (Leupeptin) inhibitors after infection (Figure 3C). When tested with overexpressed MICA or B7-H6 (ULBP2 was not tested), MG132 did not result in recovery of NK ligand levels. Leupeptin resulted in a small recovery of B7-H6; however, this was seen in both Mock and infected cells, implying that it reflects constitutive turnover of the ligand rather than virus-specific targeting, and recovery in infected cells remained reduced compared to untreated mock infected cells. Overall, these results are consistent with the virus targeting NK ligands at the level of synthesis, rather than post-translationally. We therefore investigated whether NK ligands were particularly sensitive to inhibition of synthesis, by treating cells expressing MICA, B7-H6, or a control protein (GFP) with the translation inhibitor cycloheximide (ULBP2 was not tested). This resulted in rapid loss of MICA, ULBP2, and B7-H6, but not GFP (Figure 4A). Finally, to determine which viral proteins were responsible for these effects, we transfected cells with a library of previously validated plasmids expressing all 29 SARS-CoV-2 ORFs individually (Gordon et al., 2020), and assessed them for levels of NK ligands on the cell surface. Only two SARS-CoV-2 ORFs resulted in a reduction of staining; Nsp1 and Nsp14 (Figure 4B). For MICA and ULBP2, Nsp14 consistently resulted in the strongest reduction, with Nsp1 being weaker. For Nectin1 and B7-H6, effects were weaker, but both Nsp1 and Nsp14 resulted in similar reductions.

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SARS-CoV-2 infection restricts the expression of multiple NK ligands, inhibiting NK activation.

(A) AAT cells were infected with RAd-MICA, RAd-B7-H6, or RAd-GFP, then after 24 hr either mock infected, infected with SARS-CoV-2 (SCV2), or treated with Cycloheximide (CHX). Twenty-four hr later, cells were lysed and analysed by western blot for the indicated proteins (Note: The additional band seen when staining for actin in the presence of SARS-CoV-2 may be non-specific). (B) 293T cells were transfected with expression plasmids for Nsp1 or Nsp14, or GFP as a control. Twenty-four hr later, cells were detached with HyQtase and stained for the indicated proteins. (C–E) AAT cells were either mock infected, or infected with SARS-CoV-2 for 24 h (MOI = 5), detached with TrypLE and mixed with interferon stimulated PBMC for 5 hr in the presence of golgistop, golgiplug, and CD107a antibody, before staining for CD3/CD56 and Live/Dead Aqua. Cells were then fixed, permeabilised, and stained for TNFα and IFNγ. Cells were gated on live NK cells, and the percentage of cells positive for CD107a, TNFα, and IFNγ calculated. Individual assays were run in technical triplicate, with data shown from four assays using different donors. Kruskal–Wallis, *p<0.05, **p<0.01.

Both Nsp1 and Nsp14 are involved in the ability of SARS-CoV-2 to degrade host RNA (Nsp1), and inhibit host protein translation (Nsp1 and Nsp14), and these activities have been implicated in their ability to counteract the interferon response within the infected cell (Yuan et al., 2021; Hsu et al., 2021). Together with our biochemical analysis, this data suggests that NK ligands are highly susceptible to inhibition of synthesis, and that SARS-CoV-2 manipulation of host protein production could impact the NK response in addition to the interferon response. To determine whether this was the case, we incubated SARS-CoV-2 infected cells with PBMC, and measured (i) degranulation of CD3-CD56+ NK cells (Figure 4C; Figure 3—figure supplement 2B), (ii) production of IFNγ (Figure 4D), and (iii) TNFα (Figure 4E, Figure 3—figure supplement 2B). Consistent with data using other viruses Vlahava et al., 2021, CD107a was recirculated on a higher proportion of NK cells than IFNγ and TNFα. Nevertheless, all three markers of NK activation were significantly reduced in SARS-CoV-2 infected versus mock infected cells, suggesting that the virus has evolved to inhibit NK cell responses. Comparable results were seen at both 24 hr and 48 hr post-infection (data not shown).

NK-cell evasion is overcome by antibody-dependent activation

In addition to their role in innate immunity through interactions with activating and inhibitory NK receptors early in infection, NK cells play additional roles following the development of adaptive immunity via ADNKA/ADCC, in which CD16 interacts with the Fc portion of antibodies bound to targets on the surface of infected cells. To analyse this aspect of NK function, PBMC were incubated with infected cells in the presence of sera from individuals that were seronegative or seropositive for SARS-CoV-2. Certain NK cell subsets degranulate more effectively in response to antibody bound targets. These NK cells can be differentiated by the presence of NKG2C and CD57 on the cell surface (Stary and Stary, 2020). However, NKG2C is only found in a subset of donors who are HCMV seropositive, and a proportion of these individuals are NKG2Cnull due to a deletion in the KLRC2 gene. As a result, NKG2C cannot be used as a marker in all donors. We have previously shown that CD57 is sufficient to identify higher responding NK cells in all donors, and activation of this population correlates with the ability of antibodies to mediate ADCC (Fielding et al., 2021; Wang et al., 2018). We therefore focussed on this population here; identical patterns of activation were observed in the CD57-negative population, albeit of slightly smaller magnitude (not shown). Non-specific activation was controlled for by testing against mock-infected cells throughout.

Degranulation (Figure 5A), as well as production of IFNγ (Figure 5B) and TNFα (Figure 5C), were all significantly increased in the presence of serum containing anti-SARS-CoV-2 antibodies, across a range of different NK donors (Figure 5D–F). ADNKA was dependent on virus replication, since heat inactivated virus did not lead to serum-dependent NK degranulation (Figure 5—figure supplement 1A). NK cells were activated equivalently whether purified NK cells or PBMC were used (Figure 5—figure supplement 1B), thus these responses are due to direct stimulation of NK cells, rather than other cells responding to antibodies and releasing NK stimulating cytokines. Viral immune evasion can therefore be overcome through antibody dependent mechanisms, suggesting that in addition to neutralising virus, SARS-CoV-2 antibodies may aid virus clearance through ADCC.

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SARS-CoV-2 inhibition of NK activation can be overcome via ADNKA.

AAT cells were either mock infected, or infected with SARS-CoV-2 for 24 hr (MOI = 5), detached using TrypLE, then mixed with PBMC in the presence of golgistop, CD107a antibody, and serum from donors who were seronegative or seropositive for SARS-CoV-2. After 5 hr, cells were stained for CD3, CD56, CD57, and live/dead aqua, fixed, permeabilised and stained for IFNγ and TNFα, then analysed by flow cytometry. (A–F) assays were performed using 1% serum, and the percentage of CD57 +NK cells positive for CD107a (A, D), TNFα (B, E), and IFNγ (C, F) were calculated. Individual donors were performed in technical triplicate (A–C), two-way ANOVA **p<0.01, ****p<0.0001. Assays from multiple donors were also compared (D–F), Kruskal–Wallis **p<0.01, ***p<0.001. (G–H) assays were performed using a serial threefold dilution of serum, starting from 3.3%, then the area under the curve (AUC) calculated. (G) Longitudinal serums from donors who experienced mild (black) or severe (grey) were used. (H) In addition to AUC for ADNKA activity, serums were tested for their ability to neutralise SARS-CoV-2 infection of VeroE6 cells, and the NT50 calculated, then compared to the AUC for ADNKA; Spearman rank correlation analysis is shown.

The magnitude of the neutralising antibody response to SARS-CoV-2 correlates with severity of disease; individuals with more severe disease generate higher levels of neutralising antibody (Seow et al., 2020; Dan et al., 2021; Wajnberg et al., 2020). Although memory B-cell responses persist, levels of circulating antibody can decrease over time following resolution of disease, such that in some mildly infected people they become undetectable after a few months (Seow et al., 2020; Dan et al., 2021; Wajnberg et al., 2020). To determine whether the same was true of ADCC responses, we assessed ADNKA in a cohort of donors with known disease status, for whom longitudinal sera were available (Figure 5G). ADNKA responses were generally of greater magnitude in individuals with more severe disease, with responses remaining detectable during the 150–200 day follow-up. Amongst those with milder disease, strong initial responses were maintained over time, while weaker responses decreased, becoming virtually undetectable by around 200 days. There was a moderate correlation between the ability of antibodies to neutralise SARS-CoV-2, and to activate ADNKA in response to SARS-CoV-2 (Figure 5H), with a R2 value of 0.7. Thus, although the antibodies mediating these activities may be induced in a similar manner, the antibodies mediating the two responses may not be identical.

Multiple SARS-CoV-2 ORFs mediate ADCC

There has been a major focus on spike as an activator of ADCC (Barrett et al., 2021; Herman et al., 2021; Zohar et al., 2020; Tauzin et al., 2021; Anand et al., 2021; Lee et al., 2020; Dufloo et al., 2020; Tortorici et al., 2020; McCallum et al., 2021; Cathcart et al., 2021). It is certainly a potential target during infection as, despite the fact that virion particles bud internally rather than from the cell membrane, both our PMP and flow cytometric analysis detected substantial levels of spike protein on the infected cell surface (Figure 6A, C, Figure 5—figure supplement 1C, D). However, our studies with other viruses have demonstrated that although viral proteins involved in virion binding and entry are found on the cell surface, and can be bound by antibody, they are not necessarily the strongest mediators of ADCC activity (Vlahava et al., 2021). PMP also detected membrane (M), nucleocapsid (N), ORF3a, and ORF1ab on the cell surface. ORF1ab is expressed as a polyprotein, which is proteolytically cleaved into multiple non-structural proteins (NSPs). Analysis of individual peptides representative of the proteolytically processed NSPs did not reveal any specific NSP enrichment. Furthermore, comparing the relative abundance of viral proteins in PMP, ORF1ab was only present at very low levels (1% of all viral proteins, significantly lower than any other SARS-CoV-2 protein). Taken together, it is unlikely that any NSPs are surface-exposed. Nevertheless, we performed bioinformatic analysis of all viral proteins for the presence of potential transmembrane domains or signal peptides, revealing five additional candidate cell-surface proteins (Nsp4, Nsp6, ORF6, ORF7a, ORF7b). We transfected cells with previously validated expression constructs for each of these genes (Gordon et al., 2020), and screened them for their ability to activate NK cells in an antibody-dependent manner. We used sera from patients with severe COVID-19 disease, that had previously generated the strongest ADNKA signals against infected cells (Figure 6B). As expected, spike primed a substantial ADNKA response. However, in support of our PMP data, expression of ORF3a, membrane, and nucleocapsid, were also capable of priming ADNKA in the presence of serum containing SARS-CoV-2 antibodies, while the Nsps did not.

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Multiple SARS-CoV-2 proteins mediate ADCC.

(A) Plots of viral proteins detected in the PMP analysis. (B) 293T cells were transfected with plasmids expressing the indicated SARS-CoV-2 proteins for 48 hr, then mixed with PBMC in the presence of golgistop, CD107a antibody, and 1% serum from donors who were seronegative or seropositive for SARS-CoV-2. After 5 hr, cells were stained for CD3, CD56, CD57, and live/dead aqua, then analysed by flow cytometry. Assays were performed in technical triplicate. Data is shown from two donor serums. **p<0.01, ****p<0.0001, two-way ANOVA. (C) 293T cells were transfected with plasmids expressing SARS-CoV-2 nucleocapsid or spike (or empty vector; light grey) for 48 hr, or AAT cells were infected with SARS-CoV-2 (MOI = 5) (or mock infected; light grey) for 24 hr, then cells were detached with TrypLE, stained for nucleocapsid or spike, and analysed by flow cytometry. (D) AAT cells were infected with SARS-CoV-2 (MOI = 5) for 24 hr, then cells were fixed in paraformaldehyde and stained for the indicated targets. Images are shown as maximum intensity projections of Z-stacks.

Interestingly, nucleocapsid readily activated ADNKA despite the fact that this protein has previously been assumed to be intracellular. We have previously observed the exquisite sensitivity of ADNKA, which can be activated in response to levels of virus protein that are difficult to detect by flow cytometry (Vlahava et al., 2021). We have also shown that antigen levels can be very different in the context of virus infection, compared with transfection (Vlahava et al., 2021). In support of this, nucleocapsid levels were much higher on the surface of infected cells than transfected cells (Figure 6C); detection of nucleocapsid was dependent on virus replication (Figure 5—figure supplement 1C), and occurred following infection of multiple different cell types (Figure 5—figure supplement 1D). The presence of nucleocapsid on the cell surface was also supported by immunofluorescence staining of non-permeabilised infected cells (Figure 6D), where distinct patches of nucleocapsid were observed that sometimes, but not always, coincided with cell-cell junctions. Given how well even very low levels of surface nucleocapsid activated ADNKA, this suggested that multiple proteins could be major contributors to ADCC during SARS-CoV-2 infection.

Monoclonal anti-spike antibodies only weakly mediate ADNKA despite binding strongly to infected cells

To determine the relative contribution of spike, and of different antigenic sites on spike, to ADNKA during SARS-CoV-2 infection, we tested a panel of 26 human anti-spike monoclonal antibodies, which had been isolated from naturally infected donors, and cloned as IgG1 constructs (Graham et al., 2021). The panel included antibodies targeting the RBD, S1, NTD, and S2 domains, neutralising and non-neutralising, and those targeting multiple distinct epitopes (Supplementary file 4).

All 26 antibodies bound efficiently to infected cells, at levels comparable to polyclonal serum (Figure 7—figure supplement 1). However, only five of these antibodies were capable of triggering degranulation (Figure 7A), of which two triggered TNFα (Figure 7—figure supplement 2A), and one IFNγ (Figure 7—figure supplement 2B). Importantly, these NK responses were at dramatically lower levels than seen with polyclonal serum from a naturally infected donor, even when combined (Figure 7B).

Figure 7 with 2 supplements see all
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Monoclonal anti-spike antibodies bind infected cells strongly, but only weakly activate ADNKA.

(A–B) AAT cells were either mock infected, or infected with SARS-CoV-2 (MOI = 5), or (C) 293T cells were transfected with an expression plasmid for spike. After 24 hr (A–B) or 48 hr (C), cells were detached with TrypLE and mixed with PBMC and the indicated antibodies, or a serum from a moderate case of COVID-19. Cells were incubated for 5 hr in the presence of golgistop, golgiplug, and CD107a antibody, before staining for CD3, CD56, CD57, and Live/Dead Aqua. Cells were gated on live CD57 +NK cells, and the percentage of cells positive for CD107a calculated. Assays were run in technical triplicate. Two-way ANOVA ***p<0.001, ****p<0.0001.

A number of previous publications have used cells transfected with spike expression constructs to suggest that monoclonal anti-spike antibodies can induce potent ADNKA, which would appear to contradict our data (Chan et al., 2020; Schäfer et al., 2021; Winkler et al., 2020; Suryadevara et al., 2021; Winkler et al., 2021; Yamin et al., 2021). We therefore tested a subset of our monoclonal antibodies against transfected, as opposed to infected, cells (Figure 7C). mAbs that induced ADNKA against infected cells also induced ADNKA against transfected cells (P008-014 and P054-044). However, of 12 antibodies that failed to activate ADNKA against infected cells, 5 induced potent ADNKA against transfected cells. Furthermore, in contrast to infected cells, the level of ADNKA induced by mAbs was extremely high against transfected cells, with many exceeding that of our control seropositive serum. Thus, despite a substantial ability to activate ADCC against cells transfected with spike protein, and high levels of antibody binding, spike was a relatively poor ADCC target in the context of natural infection when using mAbs.

Following natural infection, ADNKA responses are dominated by non-spike antibodies

Although our data using monoclonal antibodies indicated that spike was a comparatively weak activator of ADNKA, the antibodies that mediate ADNKA against infected cells may represent a minor proportion of the overall anti-spike response; we simply may not have captured those antibodies in our panel. To directly address this concern, we specifically depleted anti-spike antibodies from the serum of naturally infected individuals, and assessed changes in activity. ELISA analysis confirmed the effective depletion of spike-specific antibodies (Figure 8A), which was accompanied by a significant loss of neutralisation activity (Figure 8B), across multiple donors (Figure 8C). However, there was no loss of ADNKA activity from any of these samples following spike-antibody depletion (Figure 8D and E), consistent with the results of our monoclonal antibody data. We conclude that spike-specific antibodies dominate the neutralising antibody response, but play a minor roles in the ADCC response following natural infection.

Figure 8
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The ADNKA response is dominated by non-spike antibodies following natural infection.

Serums from individuals naturally infected with SARS-CoV-2 were depleted of anti-spike antibodies using spike trimer protein (A–E) or both spike trimer and nucleocapsid (G–H) conjugated to magnetic beads. (A) ELISA for spike trimer was used to measure levels of antibodies before and after depletion. (B, C) the ability of the original, or anti-spike depleted, serums to neutralise the ability of SARS-CoV-2 to infect VeroE6 cells was determined across a range of concentrations, then NT50 values calculated. Example plots (B) and NT50 values for multiple donors (C) are shown. (D–K) AAT cells were either mock infected, or infected with SARS-CoV-2 (D–H), or with SARS-CoV-2 from which ORF3 had been deleted (J, K) for 24 h (MOI = 5), detached using TrypLE, then mixed with PBMC in the presence of golgistop, CD107a antibody, and serial dilutions of serum, or monoclonal antibody. After 5 hr, cells were stained for CD3, CD56, CD57, and live/dead aqua, then analysed by flow cytometry for the percentage of CD107a-positive CD57 +NK cells. AUC values were then calculated. Example plots (D, G, J) and AUC values for multiple donors (E, H, K) are shown. Kruskal–Wallis *p<0.05, **p<0.01, ****p<0.0001. (I) Twenty-four hr after infection, cells were detached and stained for the indicated proteins after analysis by flow cytometry.

Since nucleocapsid, ORF3a, and membrane had activated ADNKA when expressed in isolation, we therefore investigated whether they also mediated ADNKA against virus infected cells. A nucleocapsid-specific human monoclonal antibody was capable of activating ADNKA against infected cells (Figure 8F) and, although not to the extent of polyclonal serum, was superior to our best anti-spike antibody. Furthermore, when we used the serums from which Spike antibodies had been depleted (Figure 8D–E), and also depleted anti-nucleocapsid antibodies, we saw a significant reduction in ADNKA activity (Figure 8G–H), although considerable activity remained. Reagents are not available to deplete antibodies targeting ORF3a or membrane, so we instead deleted ORF3a from the virus genome. In line with existing data, this resulted in a small reduction (~1 log) in virus titres (not shown). We therefore assessed levels of viral antigens on the cell surface following infection, which revealed that deletion of ORF3a resulted in a small reduction in levels of spike and nucleocapsid, but not membrane (Figure 8I). To ensure that these alterations did not affect readouts, we used serums that had been depleted for antibodies targeting both Spike and Nucleocapsid, and compared ADNKA responses against wildtype or ΔORF3a virus (Figure 8J–K). Deletion of ORF3a resulted in a dramatic reduction in ADNKA activity, although this was not reduced completely to the level of seronegative serum. We therefore conclude that nucleocapsid and ORF3a are targets for ADNKA activity against SARS-CoV-2 infected cells, with membrane potentially also contributing.

Spike-specific antibodies following vaccination are weak mediators of ADNKA

Since non-spike antigens are required to efficiently prime ADCC following natural infection, we investigated individuals who have been vaccinated against spike, but not exposed to the virus – since they will only have spike-specific antibodies. As with serum from naturally infected individuals, ELISA demonstrated effective depletion of spike antibodies (Figure 9A), which was accompanied by a marked loss of virus neutralisation activity (Figure 9B and C). In functional NK assays, these sera only mediated weak ADNKA (Figure 9D and E) and, unlike sera from naturally infected individuals, ADNKA activity was abrogated following depletion of spike antibodies. When we compared responses following natural infection or vaccination, vaccine mediated ADNKA was significantly weaker than responses seen following natural infection, and was not boosted following the second dose of the vaccine (Figure 9F). This was in stark contrast with the neutralisation activity of those same serum samples, which were dramatically increased following the second dose, with activity comparable to patients with severe COVID-19 disease (Figure 9G).

Figure 9
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Vaccine responses focussed on spike are weak activators of ADNKA.

Serums from donors that had been vaccinated against SARS-CoV-2 but were seronegative prior to vaccination were depleted of anti-spike antibodies using spike trimer protein conjugated to magnetic beads. (A) ELISA for spike trimer was used to measure levels of antibodies before and after depletion. (B, C) The ability of the original, or anti-spike depleted, serums to neutralise the ability of SARS-CoV-2 to infect VeroE6 cells was determined across a range of concentrations, then NT50 values calculated. Example plots (B) and NT50 values for multiple donors (C) are shown. (D, E) AAT cells were either mock infected, or infected with SARS-CoV-2 for 24 h (MOI = 5), detached using TrypLE, then mixed with PBMC in the presence of golgistop, CD107a antibody, and serial dilutions of serum. After 5 hr, cells were stained for CD3, CD56, CD57, and live/dead aqua, then analysed by flow cytometry for the percentage of CD107a positive CD57 +NK cells. AUC values were then calculated. All serums were additionally tested against mock infected cells to ensure no non-specific NK activation occurred (not shown). Example plots (D) and AUC values for multiple donors (E) are shown. Note that (D) is plotted on the same scale as Figure 8D to enable easy comparisons. Neutralisation (F) and ADNKA (G) values are compared for samples categorised according to disease or vaccination status. Kruskal–Wallis *p<0.05, **p<0.001.

Thus, anti-spike antibodies can mediate ADNKA, but this activity is significantly weaker than responses seen during natural infection, which targets a broader repertoire of viral antigens.

Discussion

The host innate immune system plays a critical role in both early SARS-CoV-2 infection, and later COVID-19 disease. This is emphasised by the identification of multiple interferon antagonists encoded by SARS-CoV-2, and the increased disease severity in patients with genetic and acquired interferon deficiencies (Lei et al., 2020; Sa Ribero et al., 2020; Miorin et al., 2020; Schroeder et al., 2021). To determine whether the virus evades other components of the host immune system we took a proteomic approach to systematically analyse how SARS-CoV-2 infection manipulates the cell surface during the time course of SARS-CoV-2 infection. We find that SARS-CoV-2 remodelling of the plasma membrane leads to the downregulation of multiple cell surface immune ligands involved with the interferon response, cytokine function, and NK activation. This may enable SARS-CoV-2 to evade numerous effector arms of the host immune system. Of particular interest, NK activating ligands appear particularly susceptible to viral shutoff of host gene expression, and this results in inhibition of NK cell activation. This provides an explanation for how small RNA viruses may be able to target the NK response without encoding the large repertoire of specific NK-evasins seen in the large DNA viruses (Patel et al., 2018; Berry et al., 2020), and indicates that shutdown of host transcription and translation is likely to play a broader role in the ability of the virus to establish initial infection than previously appreciated. Later in disease, following the production of antibody, ADNKA leads to significant degranulation, and production of pro-inflammatory cytokines. Somewhat surprisingly, spike antibody is not responsible for the robust ADNKA which develops after natural infection, and only weak ADNKA is generated following spike vaccination.

Genetic variation in both viruses and their hosts affects the ability of viruses to modulate NK responses, and influence pathogenesis (Patel et al., 2018; Vietzen et al., 2021a). SARS-CoV-2 appears to have evolved to antagonise NK activation by targeting the expression levels of multiple activating NK ligands. Nectin-1 is a ligand for CD96/TACTILE (Seth et al., 2007), which activates human NK cells (Holmes, 2019), B7-H6 is a ligand for NKp30, while ULBP2 and MICA are ligands for NKG2D. NKG2D is ubiquitously expressed on γδ cells and CD8+, as well as some CD4+, T-cells in addition to NK cells (Zingoni et al., 2018). Targeting NKG2D ligands may therefore enable the virus to impact additional effector mechanisms beyond just NK cells. Amongst NKG2D ligands, MICA is particularly polymorphic (100+alleles), A549 cells express MICA*001/004 (Seidel et al., 2015), while the truncated MICA*008 is the most common allele worldwide. This diversity is largely driven by co-evolution of humans with virus infections, and reflects the broad repertoire of viral immune-evasins (Zingoni et al., 2018). MICA variation therefore has the potential to play a role in the extremely variable outcome of SARS-CoV-2 infection.

A recent study demonstrated that NK cells stimulated with IL-12 and IL-15 were capable of controlling SARS-CoV-2 following infection of VeroE6 or Calu3 cells (Witkowski et al., 2021). NK ligands are highly diverse between species, thus VeroE6 (i.e. African green monkey) cells cannot inform on the ability of human NK cells to control infection, while expression levels of NK ligands were not assessed on Calu3 cells, making it difficult to draw comparisons between the studies. Nevertheless, NK cell activity is heavily dependent on stimulation, and it is highly likely that strongly activating cytokines such as IL-12/IL-15 are capable of stimulating NK cells sufficiently to contribute to viral control despite viral evasion mechanisms.

In contrast to the effect on NK ligands, we observed minimal downregulation of MHC-I, implying that SARS-CoV-2 cannot prevent peptide presentation to antagonise adaptive cytotoxic cellular immunity. Nor do infected cells bind human IgG from seronegative donors, indicating that it does not encode Fc receptors as ADNKA decoys. These observations are consistent with SARS-CoV-2 acting as an acute ‘hit and run’ virus, which has replicated and transmitted before host adaptive immunity develops. A previous study has demonstrated that SARS-CoV-2 downregulates HLA-A2 via ORF8, which contrasts somewhat with our results (Zhang et al., 2021). This may indicate a HLA- or cell-type-specific effect, it will be interesting to determine whether coronaviruses evade innate and adaptive immunity in their original bat host, and whether this contributes to their persistence in that species.

In animal models of vaccination (Gorman et al., 2021) and monoclonal antibody administration (Chan et al., 2020; Schäfer et al., 2021; Winkler et al., 2020; Suryadevara et al., 2021; Yamin et al., 2021) against SARS-CoV-2, antibody-mediated activation of cellular immunity is a critical component of the protective immune response. However, inflammation dominates severe COVID-19 disease (Horby et al., 2021b; Horby et al., 2021a). The high levels of TNFα and IFNγ observed following ADNKA are likely to exacerbate disease under these circumstances. In support of this, TNFα and IFNγ correlate with severe disease (Karki et al., 2021), and critically ill patients have a higher proportion of afucosylated antibodies that may promote a stronger ADCC response (Larsen et al., 2021). Given that ADCC can potentially contribute to both protection and immunopathology, it is important to understand what determines the magnitude and quality of the ADCC response. Viral entry glycoproteins are a common focus as mediators of ADCC. For example ADCC mediated by Env antibodies have been a major focus in HIV (Forthal and Finzi, 2018), and gB-specific antibodies have been studied in HCMV (Nelson et al., 2018). Although antibodies targeting these antigens can clearly bind infected cells, using similar approaches to the present study we recently showed that non-structural accessory proteins were more potent ADCC targets during HCMV infection (Vlahava et al., 2021). This conclusion is reinforced here, where the neutralising antibody response is dominated by spike, but spike antibodies are poor activators of ADNKA, with other viral antigens driving more efficient ADNKA following natural infection. In support of this data, monoclonal anti-spike antibodies were only effective at activating Fc-mediated control of infection in animal models following engineering to enhance activity (Beaudoin-Bussières et al., 2021). Numerous spike mAbs were capable of potently activating ADNKA against transfected cells, but failed to stimulate NK cells in the presence of infected cells; this may result from differential post-translational modifications of spike between these contexts, the fact that the reduction in activating NK ligands as a result of infection alters the threshold for activation through ADNKA, or the fact that viral CPE affects the formation of an immunological synapse (Stanton et al., 2014). Nevertheless, this underscores the importance of testing immune responses against live virus infected cells in order to assess efficacy. Importantly, the ability of monoclonal spike antibodies to bind to infected cells did not correlate with their ability to mediate ADNKA, implying that the epitope, position, or angle, at which the antibody binds to the cell surface is critical; it’s notable that 4/5 anti-spike antibodies that activated ADNKA bound the NTD, suggesting that this site is structurally advantageous for Fc-mediated responses.

A similar phenomenon may underly the greater ability of non-spike antigens to generate a ADNKA response. Nucleocapsid, ORF3a, and membrane are all present on the infected cell surface, and can promote ADNKA if appropriate antibodies are present. In some studies, antibodies targeting ORF3a and membrane have been reported as present in only a subset of patients, while almost everyone developed antibodies targeting nucleocapsid (Shrock et al., 2020). However, other studies have shown that antibodies targeting ORF3a and membrane are much more common and found in most patients (Heffron et al., 2021). Our functional work is consistent with this latter data, and suggests that antibodies targeting all three proteins may be major targets for ADCC during natural SARS-CoV-2 infection, identifying a potential functional role for antibodies binding these immunodominant targets for the first time. Although it is surprising to observe nucleocapsid on the cell surface, Influenza nucleoprotein is also detected at the plasma membrane (Yewdell et al., 1981; Fujimoto et al., 2016; Virelizier et al., 1977), and antibodies targeting nucleoprotein can mediate ADCC (Vanderven et al., 2016; Jegaskanda et al., 2017; Laidlaw et al., 2013). Structural proteins involved in genome packaging may therefore represent a common target for ADCC across multiple virus families.

The identification of the antigens that drive ADCC is important to our understanding of the mechanisms that underlie pathogenesis and disease. A skewing of the spike:nucleocapsid antibody ratio is associated with severe disease (Röltgen et al., 2020; de Campos Mata et al., 2020; Atyeo et al., 2020), where ADCC-mediating nucleocapsid antibodies might be promoting inflammation. Both the breadth of the nucleocapsid antibody response, and the particular epitopes targeted, have also been correlated with disease severity (Shrock et al., 2020; Voss et al., 2021). These correlations now need to be followed up functionally, to determine how the epitopes bound by anti-nucleocapsid antibodies affect ADCC. Critically, the same surface-bound antibodies that activate NK cells are likely to promote inflammation by activating myeloid cells and complement (Schulte-Schrepping et al., 2020; Szabo et al., 2021; Merad and Martin, 2020), both of which are hallmarks of severe COVID-19.

Although Fc-dependent mechanisms of cellular immune activation could contribute to immunopathology via multiple mechanisms in severe disease, all patients with mild/asymptomatic disease also developed strong and persistent ADNKA responses. Furthermore, lineage defining mutations in the viral variants of concern B.1.1.7, B.1.351, and P.1, include non-synonymous mutations in membrane, ORF3a, and nucleocapsid, and these can result in reduced antibody binding (Moore et al., 2021; Del Vecchio et al., 2021). Virus variants may therefore evolve to evade ADCC responses as well as neutralising responses, supporting an important role for ADCC in protection. It is therefore significant that spike-vaccine generated antibody responses were poor ADNKA inducers when tested against infected cells. The addition of other viral proteins such as nucleocapsid to vaccines would engage a wider range of immune effector pathways. This approach might improve efficacy against both viral transmission and disease, resulting in vaccines that are more resistant to viral variants containing mutations that diminish antibody neutralisation (Madhi et al., 2021).

Materials and methods

Key resources table
Reagent type (species) or resourceDesignationSource or referenceIdentifiersAdditional information
Strain, Strain Background (SARS-CoV-2)England 2Public Health EnglandGenome identical to NC_045512
Cell Line (Human)A549ATCCCCL-185
Cell Line (African Green Monkey)VeroE6ATCCCCL-81
AntibodyMouse Monoclonal anti-CD107a-FITCBiolegendCat No. 328,606Flow cytometry (1:100)
AntibodyMouse monoclonal anti-CD56-BV605BiolegendCat No. 362,538Flow cytometry (1:100)
AntibodyMouse monoclonal anti-CD3-PC7BiolegendCat No. 300,420Flow cytometry (1:100)
AntibodyMouse monoclonal anti-CD57-APCBiolegendCat No. 359,610Flow cytometry (1:100)
AntibodyMouse monoclonal anti-B7-H6BiotechneCat No. MAB7144Flow cytometry (1:50)
AntibodyMouse monoclonal anti-Nectin-1BiolegendCat No. 340,402Flow cytometry (1:200)
AntibodyMouse monoclonal anti-MICABAMOMABCat No. AMO1-100Flow cytometry (1:200)
AntibodyMouse monoclonal anti-ULBP2BAMOMABCat No. BUMO1Flow cytometry (1:200)
AntibodyMouse monoclonal anti-SpikeInsightCat No. GTX632604Flow cytometry (1:500)
AntibodyMouse monoclonal anti-NucleocapsidStratechCat No. BSM-41411MFlow Cytometry (1:300)
AntibodyGoat polyclonal anti-mouse IgG-AF647Thermo FisherCat No. A-21235Flow Cytometry (1:500)
AntibodyMouse monoclonal CD3-BV711BiolegendCat No. 344,837Flow cytometry (1:100)
AntibodyMouse monoclonal CD57-PECy7BiolegendCat No. 359,623Flow cytometry (1:100)
AntibodyMouse monoclonal TNFα–BV421BiolegendCat No. 502,931Flow cytometry (1:100)
AntibodyMouse monoclonal IFNγ–APCBiolegendCat No. 502,511Flow cytometry (1:100)
AntibodyGoat polyclonal Anti-mouse AF594ThermofisherCat No. A48288IFA (1:500)
AntibodyHuman monoclonal anti-nucleocapsidAcroCat No. NUN-S41Flow cytometry (10 μg/ml)
OtherPhalloidin-AF488ThermofisherCat No. A12379IFA (1:100)
Commercial assay, kitAnti-spikeAcroRAS-T025
Commercial assay, kitAnti-Spike Magnetic beadsAcroMBS-K015
Commercial assay, kitAnti-nucleocapsid Magnetic BeadsAcroMBS-K017

Cells and viruses

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All cell lines tested negative for mycoplasma, and A549 were authenticated by Short tandem Repeat analysis. A549 were transduced with lentiviruses expressing human ACE2, and TMPRSS2 (AAT cells), as previously described (Rihn et al., 2021). The England2 strain of SARS-CoV-2 was obtained from Public Health England (PHE), and grown on VeroE6 cells. This strain has a genome that is identical to the original Wuhan isolate. A virus lacking ORF3a, along with the parental virus, was a kind gift from Luis Martinez-Sobrido (Texas Biomedical Research Institute; Silvas et al., 2021). Virions were concentrated by pelleting through a 30% sucrose cushion to remove contaminating soluble proteins, and titrated by plaque assay on both VeroE6, AAT, and Caco2, as previously described (Rihn et al., 2021). All cell lines were grown in DMEM containing 10% FCS (Gibco), at 37 °C and in 5% CO2. Throughout the study, multiple batches of virus were used, and no overt differences were noted between them. For assays, cells were plated out the day before, then infected at a multiplicity of infection (MOI) of 5, for 1 hr on a rocking platform. The inoculum was removed, and fresh DMEM containing 2% FCS was added, then cells were incubated until the assay. To confirm that phenotypes were dependent on active virus replication, virus was inactivated by heating to 56 °C for 30 min.

Plasma membrane profiling

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Cell surface proteins were labelled essentially as described (Weekes et al., 2014; Weekes et al., 2012). Briefly, cells were incubated in a solution containing sodium periodate, aniline and aminooxy biotin to label predominantly sialic acid containing glycans at the cell surface. Cell lysates were then enriched for labelled proteins using streptavidin-agarose beads. After extensive washing trypsin was added to liberate peptides of the enriched proteins. The resulting peptide pools were dried prior to labelling with TMT reagents.

TMT labelling and clean-up

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Samples were resuspended in 21 µL 100 mM TEAB pH 8.5. After allowing to come to room temperature, 0.2 mg TMT reagents (Thermo Fisher) were resuspended in 9 µL anhydrous acetonitrile (ACN) which was added to the respective samples and incubated at room temperature for 1 hr. A 3 µL aliquot of each sample was taken and pooled to check TMT labelling efficiency and equality of loading by LC-MS. After checking each sample was at least 98% TMT labelled total reporter ion intensities were used to normalise the pooling of the remaining samples such that the final pool was as close to a 1:1 ratio of total peptide content between samples as possible. This final pool was then dried in a vacuum centrifuge to evaporate the majority of ACN form labelling. The sample was acidified to a final 0.1% Trifluoracetic Acid (TFA) (~200 µL volume) and fluoroacetic acid (FA) was added until the SDC visibly precipitated. Four volumes of ethyl acetate were then added and the sample vortexed vigorously for 10 s. Sample was then centrifuged at 15,000 g for 5 min at RT to effect phase separation. A gel loading pipette tip was used to withdraw the lower (aqueous) phase to a fresh low adhesion microfuge tube. The sample was then partially dried in a vacuum centrifuge and brought up to a final volume of 1 mL with 0.1% TFA. FA was added until the pH was <2, confirmed by spotting onto pH paper. The sample was then cleaned up by SPE using a 50 mg tC18 SepPak cartridge (Waters). The cartridge was wetted with 1 mL 100% Methanol followed by 1 mL ACN, equilibrated with 1 mL 0.1% TFA and the sample loaded slowly. The sample was passed twice over the cartridge. The cartridge was washed 3 x with 1 mL 0.1% TFA before eluting sequentially with 250 µL 40% ACN, 70% ACN and 80% ACN and dried in a vacuum centrifuge.

Basic pH reversed phase fractionation

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TMT labelled samples were resuspended in 40 µL 200 mM Ammonium formate pH10 and transferred to a glass HPLC vial. BpH-RP fractionation was conducted on an Ultimate 3,000 UHPLC system (Thermo Scientific) equipped with a 2.1 mm ×15 cm, 1.7µ Kinetex EVO column (Phenomenex). Solvent A was 3% ACN, Solvent B was 100% ACN, solvent C was 200 mM ammonium formate (pH 10). Throughout the analysis solvent C was kept at a constant 10%. The flow rate was 500 µL/min and UV was monitored at 280 nm. Samples were loaded in 90% A for 10 min before a gradient elution of 0–10% B over 10 min (curve 3), 10–34% B over 21 min (curve 5), 34–50% B over 5 mins (curve 5) followed by a 10 min wash with 90% B. 15 s (100 µL) fractions were collected throughout the run. Fractions containing peptide (as determined by A280) were recombined across the gradient to preserve orthogonality with on-line low pH RP separation. For example, fractions 1, 25, 49, 73, 97 are combined and dried in a vacuum centrifuge and stored at –20 °C until LC-MS analysis. 24 Fractions were generated in this manner.

Mass spectrometry

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Samples were analysed on an Orbitrap Fusion instrument on-line with an Ultimate 3000 RSLC nano UHPLC system (Thermo Fisher). Samples were resuspended in 10 µL 5% DMSO/1% TFA and all sample was injected. Trapping solvent was 0.1% TFA, analytical solvent A was 0.1% FA, solvent B was ACN with 0.1% FA. Samples were loaded onto a trapping column (300 µm x 5 mm PepMap cartridge trap (Thermo Fisher)) at 10 µL/min for 5 min at 60 degrees. Samples were then separated on a 75 cm x 75 µm i.d. 2 µm particle size PepMap C18 column (Thermo Fisher) at 55 degrees. The gradient was 3–10% B over 10 min, 10–35% B over 155 min, 35–45% B over 9 min followed by a wash at 95% B for 5 min and requilibration at 3% B. Eluted peptides were introduced by electrospray to the MS by applying 2.1kV to a stainless-steel emitter (5 cm x 30 µm (PepSep)). During the gradient elution, mass spectra were acquired with the parameters detailed in Figure 1—figure supplement 2 using Tune v3.3 and Xcalibur v4.3 (Thermo Fisher).

Data processing

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Data were processed with PeaksX+, v10.5 (Bioinformatic Solutions). Processing parameters are shown in detail in Figure 1—figure supplement 3. Briefly, raw files were searched iteratively in three rounds, with unmatched de novo spectra (at 1% PSM FDR) from the previous search used as the input for the next. The three iterations were as follows (1) Swissprot Human (27/03/2020)+common contaminants, (2) the same databases as search 1 but permitting semi-specific cleavage, (3) trEMBL Human (27/03/2020), with specific cleavage rules. Proteins were then quantified using the parameters outlined in Figure 1—figure supplement 3. Identified proteins and their abundances were output to.csv format and further subjected to statistical analysis. The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository (Perez-Riverol et al., 2019) with the dataset identifier PXD025000 and 10.6019/PXD025000. Supplementary file 1 contains the analysed data.

Statistical analysis and K-means clustering

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Prior to statistical analysis, proteins were filtered for those quantified across all TMT reporter channels and with more than one unique peptide. Only proteins with a plasma membrane related GO:CC annotation as previously described (Weekes et al., 2010) were then carried forward for statistical analysis. All SARS-CoV-2 proteins quantified with more than one unique peptide were also included. Statistical tests were performed with the aov and p.adjust functions in the R base stats package (version 4.0.3) to calculate Benjamini-Hochberg corrected p-values for changes in protein abundance across the measured time-points.

Proteins with a p-value <0.05 and a maximum fold-change across the time-course of >1.5-fold were selected for k-means clustering. Mean intensities of each time-point were scaled using the scale function in base R and utilised to cluster proteins into five groups using the kmeans function in R base stats package.

Functional annotation clustering

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Assessment of enriched gene annotation terms in temporal cluster one was carried out using the Functional Annotation Clustering tool at DAVID (david.ncifcrf.gov) v6.8 (Huang et al., 2009), using the default clustering settings for medium stringency and the following libraries: Uniprot UP_Keyword, GOTERM:MF_ALL, BIOCARTA, KEGG_PATHWAY and REACTOME_PATHWAY. Output clusters were curated with representative names based on the enriched terms within. The background for enrichment was a list of proteins detected in the PMP dataset with more than one peptides and a plasma membrane associated GO:CC term. Functional annotation clusters with an enrichment score of >1.5 are shown, and any clusters in which no individual annotation term had a Benjamini-Hochberg correct p-value of <0.05 were excluded.

Biochemical analysis of NK ligands

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Replication-deficient adenovirus vectors (RAd) expressing B7-H6, MICA, and ULBP2, have been described previously (Fielding et al., 2017). To analyse glycosylation of these proteins, cells were infected with RAd-MICA and RAd-GFP at MOI = 100 and RAd-B7-H6 and RAd-ULBP2 at MOI = 500, then 48 hr later cells were either mock-infected or infected with SARS-CoV-2 MOI = 5 for a further 24 hr. For some experiments, lysates were digested overnight at 37 °C with EndoH or PNGaseF (NEB) according to manufacturer’s instructions. In some experiments, MG132 (10 µM), Leupeptin (200 µM), or cycloheximide (CHX, 2.5 µg/ml) inhibitors were added 18 hr prior to harvest. Samples were then analysed by SDS-PAGE followed by western blotting for proteins of interest. Membranes were stained with primary antibodies (anti-MICA/B, BAMOMAB, BAMO1, 1:2000; anti-B7-H6, Abcam, ab121794, 1:2000; anti-ULBP-2, R&D Systems, AF1298, 1:2000; anti-GFP, FL, SC-8334, Santa Cruz Biotechnology, 1:2000; anti-Spike, Genetex, 1A9, 1:2000; anti-Actin, Sigma, 1:2000), followed by anti-mouse, anti-rabbit or anti-goat HRP-conjugated secondary antibody, then developed using Supersignal West Pico, and imaged in a Syngene XX6.

Immunofluorescence

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Cells were plated on coverslips, then infected with SARS-CoV-2. After 24 hr, cells were fixed with 4% paraformaldehyde, then stained with nucleocapsid mAb (1C7), followed by anti-mouse AF594, Phalloidin-AF488, and DAPI. Coverslips were washed, then mounted in prolong gold anti-fade (Thermofisher), before being imaged on a Zeiss LSM800 confocal microscope.

Flow cytometry

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Cells were dissociated using HyQtase, then stained with primary antibody for 30 min at 4 °C. Following washing, they were incubated with secondary antibody, again for 30 min at 4 °C. Cells were washed, fixed in 4% paraformaldehyde, and data collected on an Attune cytometer (Thermofisher). In one experiment (Figure 1A), cells were fixed and permeabilised (Cytofix/Cytoperm, BD) before staining. Antibodies used were against HLA-ABC (W632; AbD Serotec), NCR3LG1/B7-H6 (MAB7144, Biotechne R&D Systems), Nectin 1 (R1.302; Biolegend), MICA (AMO1-100; BAMOMAB), MICB (BMO2-100; BAMOMAB), ULBP2 (BUMO1; BAMOMAB), Spike (1A9; Insight), Nucleocapsid (1C7; Stratech), anti-mouse IgG AF647 (Thermofisher).

ELISA

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To measure levels of spike antibodies, an ELISA using the spike trimer (Acro Biosystems) was used according to manufacturers instructions. Each sample was measured in duplicate, and compared to a standard curve. A pre-pandemic serum was included in each assay to define the cut-off.

Plasmids and transfections

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Lentivirus plasmids encoding each SARS-CoV-2 ORF individually, with a C-terminal twin-strep tag, were obtained from Addgene, and have been validated for expression previously (Gordon et al., 2020). Plasmids were midiprepped (Nucleobond Xtra Midi; Machery-Nagel), and transfected into 293T cells using GeneJuice (Merck) according to manufacturers’ instructions.

NK activation assays

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PBMCs from healthy donors were thawed from liquid N2 storage, rested overnight in RPMI supplemented with 10% FCS, and L-glutamine (2 mM). For assays investigating viral inhibition of NK activation, PBMC were stimulated overnight with IFN-α (1,000 U/ml) to provide a baseline level of activation against which inhibition could be seen. For ADNKA this stimulation is not required, and so cells were used unstimulated (Vlahava et al., 2021). To confirm that phenotypes were due to direct effects on NK cells, NK cells were purified by depletion, using the human NK cell purification kit (Miltenyi) according to manufacturer’s instructions. Target cells were harvested using TrypLE Express (Gibco), preincubated for 30 min with the relevant antibody or serum preparations, then mixed with effectors at an effector:target (E:T) ratio of 10:1 (PBMC) or 1:1 (purified NK cells) in the presence of golgistop (0.7 μl/ml, BD Biosciences), Brefeldin-A (1:1000, Biolegend) and anti-CD107a–FITC (clone H4A3, BioLegend). Cells were incubated for 5 hr, washed in cold PBS, and stained with live/dead Fixable Aqua (Thermo Fisher Scientific), anti-CD3–PECy7 (clone UCHT1, BioLegend) or anti-CD3-BV711 (Clone SK7, Biolegend), anti-CD56–BV605 (clone 5.1H11, BioLegend), anti-CD57–APC (clone HNK-1, BioLegend) or anti-CD57-PECy7 (clone HNK-1, Biolegend), and anti-NKG2C–PE (clone 134591, R&D Systems). In some experiments, cells were also fixed/permeabilized using Cytofix/Cytoperm (BD Biosciences) and stained with anti-TNFα–BV421 (clone MAb11, BioLegend) and anti-IFNγ–APC (clone 4 S.B3, BioLegend). Data were acquired using an AttuneNxT (Thermo Fisher) and analyzed with Attune NxT software or FlowJo software version 10 (Tree Star). Individual assays were run in technical triplicate. To ensure inter-assay variation did not affect results, a donor serum demonstrating moderate ADCC activity against SARS-CoV-2 was included as a positive standard in every assay, while a serum collected before 2020 was included as a negative control in every assay. Where sera were tested at a range of dilutions, the area under the curve (AUC) was calculated using Graphpad Prism 9. This value was then normalised to the AUC for the standard serum in that particular assay. In all experiments, serums were additionally tested against mock infected cells to control for non-specific activation of NK cells by serum components. An example gating strategy is shown in Figure 4—figure supplement 1, and involved gating on NK cells defined as CD56 positive, CD3 negative. This strategy will capture all ‘classical’ NK cells; however, it will miss the recently described CD56-negative NK cell population. Since these are only present at substantial levels in specific conditions such as chronic HIV infection, and older people with HCMV or EBV infection (Müller-Durovic et al., 2019), and have not been described to expand during SARS-CoV-2 infection, they are unlikely to play a major role in the NK response examined herein.

Serums

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Serums were collected from healthy donors vaccinated with the BNT162b2 vaccine, a minimum of 3 weeks after the first dose, or 1 week after the second. A pre-vaccine sample was taken from every donor, and an ELISA for SARS-CoV-2 RBD performed as previously described (Amanat et al., 2020), to determine pre-exposure to live SARS-CoV-2. Longitudinal serums from naturally infected individuals have been described previously, clinical characteristics are in Supplementary file 3; (Seow et al., 2020). Those labelled ‘mild’ had severity scores of 0, 1, 2 or 3, and ‘severe’ was 4 or 5, as described previously (Seow et al., 2020).

Virus neutralisation assays

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600PFU of SARS-CoV-2 was incubated with appropriate dilutions of serum, in duplicate, for 1 hr, at 37 °C. The mixes were then added to pre-plated VeroE6 cells for 48 hr. After this time, monolayers were fixed with 4% PFA, permeabilised for 15 min with 0.5% NP-40, then blocked for 1 hr in PBS containing 0.1% Tween (PBST) and 3% non-fat milk. Primary antibody (anti-nucleocapsid 1C7, Stratech, 1:500 dilution) was added in PBST containing 1% non-fast milk and incubated for 1 hr at room temperature. After washing in PBST, secondary antibody (anti-mouse IgG-HRP, Pierce, 1:3000 dilution) was added in PBST containing 1% non-fat milk and incubated for 1 hr. Monolayers were washed again, developed using Sigmafast OPD according to manufacturers’ instructions, and read on a Clariostar Omega plate reader. Wells containing no virus, virus but no antibody, and a standardised serum displaying moderate activity were included as controls in every experiment. NT50 were calculated in Graphpad Prism 9.

Antibody depletions

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Anti-spike or anti-nucleocapsid antibody was depleted from sera using magnetic bead conjugated spike trimer protein or nucleocapsid protein (Acrobiosystems). Beads were resuspended in PBS +0.05% BSA, then 50 µl serum was mixed with 150 µl beads. Mixtures were incubated on a rotating mixer at 4 °C overnight. Serum diluted in buffer alone was used as a control. Magnetic beads were then removed using a 3D printed magnetic stand. All values given in assays are corrected for this initial fourfold dilution. Levels of anti-spike antibody were measured using a spike trimer ELISA (Acrobiosystems).

Data availability

All data generated are presented within the manuscript, with the exception of proteomics data. This has been uploaded to PRIDE (identifier PXD025000).

The following data sets were generated

References

    1. Orange JS
    (2013) Natural killer cell deficiency
    The Journal of Allergy and Clinical Immunology 132:515–525.
    https://doi.org/10.1016/j.jaci.2013.07.020
    1. Weekes MP
    2. Antrobus R
    3. Lill JR
    4. Duncan LM
    5. Hör S
    6. Lehner PJ
    (2010)
    Comparative analysis of techniques to purify plasma membrane proteins
    Journal of Biomolecular Techniques 21:108–115.
    1. Yewdell JW
    2. Frank E
    3. Gerhard W
    (1981)
    Expression of influenza A virus internal antigens on the surface of infected P815 cells
    Journal of Immunology (Baltimore, Md 126:1814–1819.

Decision letter

  1. Stipan Jonjic
    Reviewing Editor; University of Rijeka, Croatia
  2. Betty Diamond
    Senior Editor; The Feinstein Institute for Medical Research, United States
  3. Domenico Mavilio
    Reviewer; Humanitas Clinical and Research Center, Italy

In the interests of transparency, eLife publishes the most substantive revision requests and the accompanying author responses.

Decision letter after peer review:

Thank you for submitting your article "ADNKA overcomes SARS-CoV2-mediated NK cell inhibition through non-spike antibodies" for consideration by eLife. Your article has been reviewed by 3 peer reviewers, and the evaluation has been overseen by a Reviewing Editor and Betty Diamond as the Senior Editor. The following individual involved in review of your submission has agreed to reveal their identity: Domenico Mavilio (Reviewer #2).

The reviewers have discussed their reviews with one another, and the Reviewing Editor has drafted this to help you prepare a revised submission.

Essential revisions:

All three reviewers concluded that the manuscript provides novel insights into the SARS CoV-2 escape from natural killer (NK) cells signaling by downregulating the expression of cellular ligands for activating NK receptors. The authors also showed that strong antibody-dependent NK cell activation (ADNKA) developed later in the disease process and that ADNKA was primarily driven by viral proteins other than Spike. This aspect might be relevant as the COVID-19 vaccine currently in use are based on spike protein. The reviewers agreed that your findings would be of interest to scientists in the field of NK cell biology and SARS CoV-2 pathogenesis. However, although enthusiastic with the bulk results, the reviewers pointed out several specific aspects of the work that need to be addressed experimentally and/or clarified in the revised manuscript.

Reviewer #1:

The study 'ADNKA overcomes SARS-CoV2-mediated NK cell inhibition through non-spike antibodies' by Fielding et al. deals with NK cell response to SARS-CoV-2 infected cells. First, by using mass spectrometry the authors show altered cell surface protein expression on SARS-CoV-2-infected lung epithelial cells and downregulation of activating NK ligands such as B7-H6, MICA, ULBP2, and Nectin1. This regulation of NK cell ligands resulted in impaired NK cell response (reduction in NK cell degranulation, IFNγ, and TNFα production) towards SARS-CoV-2 infected cells. Furthermore, they show strong NK cell activation in the presence of serum containing anti-SARS-CoV2 antibodies, and that such response was sustained for up to 6 months following infection. Based on the plasma membrane profiling and the use of transfectants expressing SARS-CoV-2 proteins, they identified Spike, Nucleocapsid, Membrane, and ORF3a as potential targets for ADCC. Depleting spike-specific antibodies from sera confirmed their dominant role in neutralization, but these antibodies had a minor role in ADNKA compared to antibodies targeting other proteins. In agreement with this observation, ADNKA was weaker following vaccination as compared to natural infection and was not boosted by the second dose of vaccine. Overall, this is an important and well-performed study. However, several questions remained unanswered, which required additional experiments.

1. Only one cell line was used to analyze the regulation of NK cell ligands upon SARS-CoV-2 infection. Are the same NK ligands regulated by SARS-CoV-2 in other permissive cell lines?

2. It was published recently that NK cells can regulate SARS-CoV-2 fitness in cell culture (Witkowski et al. 2021 Nature). This paper should be cited and discussed in the context of the presented results.

3. Antibodies to Spike, Nucleocapsid, Membrane, and ORF3a induced NK cell activation when transfectants expressing respective proteins were used. Depletion of Spike antibodies did not affect NK cell activation when co-incubated with SARS-CoV-2-infected cells, and the conclusion was drawn that non-spike antibodies mediate NK cell activation. However, formal proof for this claim is missing. The experiment should be performed with depletion of some non-spike antibodies (Nucleocapsid, Membrane, or ORF3a) from sera to prove that their elimination will impair NK cell activation. This would be especially important for antibodies targeting Nucleocapsid as they are present in most individuals infected with SARS-CoV-2.

4. Spike antibodies can induce ADNKA when Spike is expressed in the context of transfection. The major question is why these antibodies do not provide ADNKA in the context of infection? They can bind to the surface of infected cells, so something interferes with their function.

5. Panel of Spike-specific monoclonal antibodies was used to show that Spike-directed antibodies poorly activate NK cells when bound to infected cells. However, it is not clear if these antibodies can provide ADCC at all. Were they tested for ADCC when co-incubated with Spike-expressing transfectants?

Reviewer #2:

This paper is of high interest to a broad audience as it provides new knowledge on the role of NK cells in the control of COVID-19 disease progression and vaccine efficacy and design.

First, the authors identified a novel mechanism by which SARS-CoV2 antagonises innate immunity. By performing a proteomic analysis of SARS-CoV-2- infected lung epithelial cells, the authors demonstrated that SARS-CoV-2 infection induced a downregulation of activating NK ligands. in vitro functional assays demonstrated that this correlated with reduction in NK cell activation. Moreover, they showed that strong antibody-dependent NK cell activation (ADNKA) developed later in the disease process. In particular, it was shown that ADNKA is not primarily driven by spike antibody but was dominated by antibody to other viral proteins such as nucleocapsid, membrane and ORF3a. Since the anti-COVID-19 vaccine currently in use is based on spike protein, this last aspect could be highly relevant in vaccine design and improvement of vaccine efficacy.

Although the manuscript is well-structured and the data analysis is rigorous and justify the conclusions, some major points deserve to be better addressed or developed.

To further extend the knowledge provided on the role of NK cells in the context of SARS-CoV-2 infection, the authors should also investigate the function of the different NK subsets. In particular, also CD56-/CD16+ NK cells – which are not included in the present analysis – deserve to be deeply investigated as these cells are greatly expanded during viral infection (Di Vito C, et al. Front Immunol. 2019. Doi:10.3389/fimmu.2019.01812).

Moreover, as at 48h after infection the activating NK ligands are still downregulated whereas HLA molecules increased their expression, functional assay could be performed also with AAT cells infected for 48h to investigate also at this time point the NK activity.

Finally, since NK activation depends also on the expression levels of activating and inhibitory NK cell receptors, it could be interesting to assess whether SARS-CoV-2 infection can modulate receptor expression on NK cells obtained from COVID-19 patients. In particular, it would be interesting to investigate the expression of NKp30 (receptor of B7-H6) and NKG2D (receptor for ULBP2 and MICA). Moreover, as an increased expression of HLA molecules was observed in infected cells at 48h it would be interesting to assess also the expression on KIRs.

– In the present study, NK cells were identified in viable CD3-/CD56+ cells and then the authors focused their attention on CD57+ NK cells (CD3-/CD56+). Nevertheless, this gating strategy has some weakness since: (i) it does not allow a specific exclusion of B cells from the analysis (CD19 should be added in the panel); (ii) it not includes CD16. This last aspect is particularly relevant since the absence of CD16 in the panel used in functional assays precludes the possibility of investigating the function of the different NK subsets. In particular, CD56-/CD16+ NK cells which are greatly expanded during viral infection (Di Vito C, et al. Front Immunol. 2019. doi:10.3389/fimmu.2019.01812) cannot be assessed with the gating strategy used. Considering the role of CD56-/CD16+ NK cells in viral infection and the crucial involvement of CD16 in ADNKA, an additional set of experiments using an improved gating strategy that include at least CD16 should be performed to address these aspects.

– At 48h after infection, activating NK ligands are still downregulated whereas HLA molecules increase their expression. Since these last ones could be either activating or inhibitory, do the Authors assessed the NK activity also in assays performed co-culturing NK cells with AAT cells infected for 48h?

Reviewer #3:

Fielding et al. have studied the response of NK cells towards SARS-CoV-2 infected cells. In an unbiased proteomics approach the cell surface proteome was determined by plasma membrane profiling, applying extracellular aminooxy-biotinylation to selectively isolate and measure surface proteins. The authors found regulation of NK cell ligands: ULBP2, Nectin1, MICA and B7-H6. MHC-I proteins, which can act as inhibitory NK cell ligands, were not downregulated. Four viral proteins (S, M, N and ORF3a) were expressed on the plasma membrane of SARS-CoV-2 infected cells. Whereas the authors describe regulation of activating NK cell ligands rather briefly, they found compelling evidence of ADCC forwarded by antibody recognition of surface expressed SARS-CoV-2 antigens. Interestingly, this effect was not primarily conducted by anti-Spike antibodies, but antibodies directed against the other cell surface antigens. This demonstrates that antibodies directed against other proteins than Spike can induce an antiviral response through NK cell mediated ADCC.

Figure 3: MICA, ULBP2, B7-H6 and Nectin1 were found to be downregulated by SARS-CoV-2 infection. The authors suggest that Nectin1 specificity by SARS-CoV-2 could be a preserved function between an original bat virus and the pandemic virus studied here, since Nectin1 from human and a bat species is well conserved. However, the authors did not study at which level the observed regulation of Nectin1 takes place. It is well established that in SARS-CoV-2 infected cells the Nsp1 proteins blocks translational activity of ribosomes. Therefore, the regulation of NK cell ligands might not be protein specific, but the result of a more general mechanism executed e.g. by Nsp1. Therefore, the level (i.e. pre-transcriptional or pre- or post -translational) at which NK cell ligands are regulated should be determined.

Transcriptional analysis would also help to understand the potential role of these NK ligands for the SARS-CoV-2 antiviral response, since neither the non-treated nor the heat-inactivated virus did induce expression of NK cell ligands at any time.

Lastly, to this part, it is not clear which trigger is decisive for reduced NK cell activation upon co-culture with SARS-CoV-2 infected cells. Blocking antibodies should be included to demonstrate the specificity and relation between Figure 3A and 3B.

Figure 5A: Is there a difference between this figure and figure 1C other than the addition of N and M proteins; could these be merged to one figure?

Figure 5B: In this figure the strongest ADNKA responses are directed against Spike using serum from two individuals. This does not fit with the results shown in Figure 7. The authors should discuss this controversy.

A previous study (Hachim, Kavian et al., NatImm 2020) showed robust antibody responses against ORF8. One can guess that ORF8 was excluded from this analysis, because the gene might be disrupted in the strain used here (England2). However, this is not mentioned and no reference for the sequence is given. This information is important and should be included. The issue with ORF8 is also of importance for regulation of MHC-I, since in a previous study ORF8 was shown to block MHC-I by lysosomal degradation (Zhang et al., PNAS 2021).

Figure 5C. The finding that the N protein can be detected on the surface of SARS-CoV-2 infected cells is surprising. Has this been reported of before for SARS-CoV-2 or other corona viruses? Is N surface expression found on all infected cells or a specific population of cells (e.g. dying cells)? If N is distributed to the surface only in dying cells it questions the importance of this antigen in triggering ADCC.

Figure 6. The authors show that recombinant anti-Spike antibodies bind to SARS-CoV-2 infected cells as determined by flow cytometry, but do not induce ADNKA. This could be due to structural hindrance. Is there an association between antibody epitope recognition and induction of ADNKA? Furthermore, is it possible to further induce CD16 activation by combining anti-S antibodies with varying specificities in one sample to mimic the situation in serum?

Figure 7. In this figure it is elegantly demonstrated that non-spike SARS-CoV-2 specific antibodies prime cells for ADNKA. Since new SARS-CoV-2 variants escape neutralization by mutation of the Spike protein, it would be relevant to know whether the ability to induce ADNKA still remains equally effective by sera from individuals infected with previous variants

This study demonstrates the ability of non-Spike SARS-CoV-2 antibodies to induce ADCC. It is indeed surprising that depletion of anti-Spike antibodies has only a modest effect on ADCC. To underline the reduced role of Spike in ADNKA, controls suggested for Figure 6 and 7 should be included. Furthermore, if this is the first report on N protein expression on the cell surface, this point should be strengthened, e.g. by immunofluorescence microscopy.

Premature data in Figure 2-3 appear rather distracting here. In my opinion the manuscript would gain in clarity if the ADNKA part would be handled on its own.

[Editors' note: further revisions were suggested prior to acceptance, as described below.]

Thank you for resubmitting your work entitled "SARS-CoV-2 host-shutoff impacts innate NK cell functions, but antibody-dependent NK activity is strongly activated through non-spike antibodies" for further consideration by eLife. Your revised article has been evaluated by Betty Diamond (Senior Editor) and a Reviewing Editor.

The manuscript has been improved but there are some remaining issues that need to be addressed, as outlined below:

Reviewer #1:

The authors have addressed all of my concerns in the revised version of the manuscript. I would suggest accepting the manuscript for publication.

Reviewer #2:

In this revised version of the manuscript by Fielding and colleagues titled "SARS-CoV-2 host-shutoff impacts innate NK cell functions, but antibody-dependent NK activity is strongly activated through non-spike antibodies", the Authors remarkably improved the quality of their manuscript, providing new data and discussing critical aspects thus addressing the Reviewers' comments and improving the scientific significance of the paper.

Reviewer #3:

The revised version of the manuscript by Fielding et al. has been much improved. It now contains more detailed data on the regulation of NKG2D and Nkp30 ligands. The authors investigated the level of regulation and were able to exclude the degradation and retention of these proteins. Instead, Fielding et al., describe a selective regulation by Nsp14 and also Nsp1. This clearly strengthens the manuscript.

In general, the points raised previously have been approached and discussed appropriately.

I recommend publication of the manuscript after the following change to the manuscript have been made.

One important control is still missing in Figure 6C: a control with uninfected cells should be included.

https://doi.org/10.7554/eLife.74489.sa1

Author response

Reviewer #1:

The study 'ADNKA overcomes SARS-CoV2-mediated NK cell inhibition through non-spike antibodies' by Fielding et al. deals with NK cell response to SARS-CoV-2 infected cells. First, by using mass spectrometry the authors show altered cell surface protein expression on SARS-CoV-2-infected lung epithelial cells and downregulation of activating NK ligands such as B7-H6, MICA, ULBP2, and Nectin1. This regulation of NK cell ligands resulted in impaired NK cell response (reduction in NK cell degranulation, IFNγ, and TNFα production) towards SARS-CoV-2 infected cells. Furthermore, they show strong NK cell activation in the presence of serum containing anti-SARS-CoV2 antibodies, and that such response was sustained for up to 6 months following infection. Based on the plasma membrane profiling and the use of transfectants expressing SARS-CoV-2 proteins, they identified Spike, Nucleocapsid, Membrane, and ORF3a as potential targets for ADCC. Depleting spike-specific antibodies from sera confirmed their dominant role in neutralization, but these antibodies had a minor role in ADNKA compared to antibodies targeting other proteins. In agreement with this observation, ADNKA was weaker following vaccination as compared to natural infection and was not boosted by the second dose of vaccine. Overall, this is an important and well-performed study. However, several questions remained unanswered, which required additional experiments.

1. Only one cell line was used to analyze the regulation of NK cell ligands upon SARS-CoV-2 infection. Are the same NK ligands regulated by SARS-CoV-2 in other permissive cell lines?

We have now investigated the use of Caco2 and Calu3, which are the only other permissive cell types in wide use for SARS-COV2 infection. We were unable to get reliable high MOI infection of Calu3, despite subcloning high ACE2 expressing cells. We have therefore analysed NK ligand downregulation in Caco2 cells. Levels of NK ligands were lower in these cells as compared to A549, nevertheless a similar downregulation was observed (new Figure S3). See also downregulation of NK ligands in 293T cells by viral ORFs that we added in response to reviewer 3’s comments.

2. It was published recently that NK cells can regulate SARS-CoV-2 fitness in cell culture (Witkowski et al. 2021 Nature). This paper should be cited and discussed in the context of the presented results.

We have now added discussion of this point: Recently a study demonstrated that NK cells stimulated with IL-12 and IL-15 were capable of controlling SARS-CoV-2 following infection of VeroE6 or Calu3 cells62. NK ligands are highly diverse between species, thus VeroE6 (i.e. African green monkey) cells cannot inform on the ability of human NK cells to control infection, while the expression levels of NK ligands were not assessed on Calu3 cells, making it difficult to draw comparisons between the studies. Nevertheless, NK cell activity is heavily dependent on stimulation, and it is highly likely that strongly activating cytokines such as IL12/IL-15 are capable of stimulating NK cells sufficiently to contribute to viral control despite viral evasion mechanisms.

3. Antibodies to Spike, Nucleocapsid, Membrane, and ORF3a induced NK cell activation when transfectants expressing respective proteins were used. Depletion of Spike antibodies did not affect NK cell activation when co-incubated with SARS-CoV-2-infected cells, and the conclusion was drawn that non-spike antibodies mediate NK cell activation. However, formal proof for this claim is missing. The experiment should be performed with depletion of some non-spike antibodies (Nucleocapsid, Membrane, or ORF3a) from sera to prove that their elimination will impair NK cell activation. This would be especially important for antibodies targeting Nucleocapsid as they are present in most individuals infected with SARS-CoV-2.

We have now depleted nucleocapsid antibodies, and shown that this impacts ADNKA. We have also used a monoclonal nucleocapsid antibody to show that this antigen is a ADNKA target. We do not have the reagents to deplete ORF3a or Membrane and cannot delete Membrane from the viral genome. However, we were able to delete ORF3a from the viral genome, and demonstrate that this results in a major reduction in ADNKA. This provides formal proof that both Nucleocapsid and ORF3a can act as ADNKA targets, and has been added to the manuscript (Figure 8).

4. Spike antibodies can induce ADNKA when Spike is expressed in the context of transfection. The major question is why these antibodies do not provide ADNKA in the context of infection? They can bind to the surface of infected cells, so something interferes with their function.

We agree that this is an important question, however addressing it is a substantial body of work in its own right, involving extensive structural and immunological work. It is therefore beyond the scope of this paper and does not change our major conclusion – that Spike mAbs are not the main mediators of ADNKA, and that all studies that have used Spike transfected cells to assess function should be re-interpreted in this light. We therefore state in the text ‘Numerous Spike mAbs were capable of potently activating ADNKA against transfected cells, but failed to stimulate NK cells in the presence of infected cells; this may result from differential post-translational modifications of Spike between these contexts, the fact that the reduction in activating NK ligands as a result of infection alters the threshold for activation through ADNKA, or the fact that viral CPE affects the formation of an immunological synapse70. Nevertheless, this underscores the importance of testing immune responses against live virus infected cells in order to assess efficacy’.

5. Panel of Spike-specific monoclonal antibodies was used to show that Spike-directed antibodies poorly activate NK cells when bound to infected cells. However, it is not clear if these antibodies can provide ADCC at all. Were they tested for ADCC when co-incubated with Spike-expressing transfectants?

We have now performed ADNKA assays using Spike transfected cells. This shows that antibodies that mediate ADNKA against infection also act against transfected cells. However numerous antibodies that do not act against infected cells, do act against transfected cells. This strengthens our conclusion that antibodies must be tested against virus, as opposed to transfection systems.

Reviewer #2:

[…]

– In the present study, NK cells were identified in viable CD3-/CD56+ cells and then the authors focused their attention on CD57+ NK cells (CD3-/CD56+). Nevertheless, this gating strategy has some weakness since: (i) it does not allow a specific exclusion of B cells from the analysis (CD19 should be added in the panel); (ii) it not includes CD16. This last aspect is particularly relevant since the absence of CD16 in the panel used in functional assays precludes the possibility of investigating the function of the different NK subsets. In particular, CD56-/CD16+ NK cells which are greatly expanded during viral infection (Di Vito C, et al. Front Immunol. 2019. doi:10.3389/fimmu.2019.01812) cannot be assessed with the gating strategy used. Considering the role of CD56-/CD16+ NK cells in viral infection and the crucial involvement of CD16 in ADNKA, an additional set of experiments using an improved gating strategy that include at least CD16 should be performed to address these aspects.

It is not possible to include a CD16 gate in functional assays, because following Fc engagement CD16 is cleaved from the cell surface in order to permit serial engagement. Thus, CD16 positive cells become CD16 negative following ADNKA. Furthermore, although the CD56-/CD16+ population has been demonstrated to be important in certain situations, this largely involves those with HIV and very elderly HCMV/EBV seropositive individuals. Since our donors do not fall into these groups, the proportion of CD56-/CD16+ cells will be too low to permit effective analysis. As a result, it is not possible to assess the role of this population in our study. We have therefore added to the methods section ‘This strategy will capture all ‘classical’ NK cells, however it will miss the recently described CD56 negative NK cell population. Since these are only present at substantial levels in specific conditions such as chronic HIV infection, and older people with HCMV or EBV infection88, and have not been described to expand during SARS-CoV2 infection, they are unlikely to play a major role in the NK response examined herein.’

– At 48h after infection, activating NK ligands are still downregulated whereas HLA molecules increase their expression. Since these last ones could be either activating or inhibitory, do the Authors assessed the NK activity also in assays performed co-culturing NK cells with AAT cells infected for 48h?

Comparable results were seen at both 24h and 48h post-infection, and we now state as such in the manuscript.

Reviewer #3:

Fielding et al. have studied the response of NK cells towards SARS-CoV-2 infected cells. In an unbiased proteomics approach the cell surface proteome was determined by plasma membrane profiling, applying extracellular aminooxy-biotinylation to selectively isolate and measure surface proteins. The authors found regulation of NK cell ligands: ULBP2, Nectin1, MICA and B7-H6. MHC-I proteins, which can act as inhibitory NK cell ligands, were not downregulated. Four viral proteins (S, M, N and ORF3a) were expressed on the plasma membrane of SARS-CoV-2 infected cells. Whereas the authors describe regulation of activating NK cell ligands rather briefly, they found compelling evidence of ADCC forwarded by antibody recognition of surface expressed SARS-CoV-2 antigens. Interestingly, this effect was not primarily conducted by anti-Spike antibodies, but antibodies directed against the other cell surface antigens. This demonstrates that antibodies directed against other proteins than Spike can induce an antiviral response through NK cell mediated ADCC.

Figure 3: MICA, ULBP2, B7-H6 and Nectin1 were found to be downregulated by SARS-CoV-2 infection. The authors suggest that Nectin1 specificity by SARS-CoV-2 could be a preserved function between an original bat virus and the pandemic virus studied here, since Nectin1 from human and a bat species is well conserved. However, the authors did not study at which level the observed regulation of Nectin1 takes place. It is well established that in SARS-CoV-2 infected cells the Nsp1 proteins blocks translational activity of ribosomes. Therefore, the regulation of NK cell ligands might not be protein specific, but the result of a more general mechanism executed e.g. by Nsp1. Therefore, the level (i.e. pre-transcriptional or pre- or post -translational) at which NK cell ligands are regulated should be determined.

We have now performed biochemical analysis to understand how SARS-CoV2 is manipulating these ligands (Figure 3-4). This revealed that there is likely a block at the stage of synthesis, as opposed to degradation or retention. Further screening of the entire SARS-CoV2 ORFeome identified that Nsp14 is likely the primary driver of this process, with some contribution from Nsp1. Given that these proteins target both transcription and translation of host genes, it seems likely that this is an example of the virus achieving inhibition of NK cell activation through non-specific targeting of host cell gene expression. This is extremely interesting, and explains how small RNA viruses are able to target the NK response without encoding a vast repertoire of specific NK modulators, as the large DNA viruses do. Given these results, we have removed the section on bat ligands from the results.

Transcriptional analysis would also help to understand the potential role of these NK ligands for the SARS-CoV-2 antiviral response, since neither the non-treated nor the heat-inactivated virus did induce expression of NK cell ligands at any time.

As described above, we have now performed extensive biochemical analysis, and screened all SARS-CoV-2 ORFs to identify viral inhibition of host gene expression as the likely mechanism by which this occurs.

Lastly, to this part, it is not clear which trigger is decisive for reduced NK cell activation upon co-culture with SARS-CoV-2 infected cells. Blocking antibodies should be included to demonstrate the specificity and relation between Figure 3A and 3B.

We have attempted to perform these experiments using antibodies to MICA, ULBP2, and B7H6 that have been shown to block NK activation; such blocking antibodies to Nectin1 have not, to our knowledge, been reported in the literature. However, it was not possible to block the activity of activating NK ligands in the presence of virus, since the ligand is already downregulated by the virus, leaving nothing to block. Nor does blocking work in the absence of virus infection; this is in line with our extensive experience analysing the NK response to other virus infections and in other cell types (e.g. 10.1371/journal.ppat.1004058, 10.7554/eLife.22206). We have consistently found that the only way to demonstrate the impact of an activating ligand in the context of virus infection is to delete the viral gene that inhibits it. When you do this, infection occurs in the presence of increased NK activating ligand that hyper-activates the NK cell response, and this can be blocked with blocking antibodies. However, with SARS-CoV-2, we cannot remove Nsp14 without inactivating the virus. At this point, it is therefore not technically possible to formally demonstrate this point, and have therefore been careful to avoid claiming anywhere that we have directly shown that these ligands are responsible for the effects seen.

Figure 5A: Is there a difference between this figure and figure 1C other than the addition of N and M proteins; could these be merged to one figure?

Although the data is the same, we would prefer to avoid introducing the presence of ORF1ab and Nucleocapsid in the earlier figure, because introducing them requires an extensive explanation of their significance on the cell surface. It is therefore easier for the text flow to keep the figures as they are originally.

Figure 5B: In this figure the strongest ADNKA responses are directed against Spike using serum from two individuals. This does not fit with the results shown in Figure 7. The authors should discuss this controversy.

We have now added an additional figure that directly demonstrates the differential impact of ADCC in the context of transfection vs infection (Figure 7C). The differential activity of antibodies against infection vs transfection may be due to glycosylation, viral CPE affecting synapse formation, or be a consequence of the viral manipulation of innate NK ligands that we describe. An extensive program of structural, virological and immunological work will be needed to investigate this, and other, hypotheses properly. At this stage our major conclusion is that other antigens are far more potent than Spike as ADNKA targets, and that immune responses need to be assessed against live virus infected cells. We therefore state

in the text: ‘this may result from differential post-translational modifications of Spike between these contexts, the fact that the reduction in activating NK ligands as a result of infection alters the threshold for activation through ADNKA, or the fact that viral CPE affects the formation of an immunological synapse70. Nevertheless, this underscores the importance of testing immune responses against live virus infected cells in order to assess efficacy.’

A previous study (Hachim, Kavian et al., NatImm 2020) showed robust antibody responses against ORF8. One can guess that ORF8 was excluded from this analysis, because the gene might be disrupted in the strain used here (England2). However, this is not mentioned and no reference for the sequence is given. This information is important and should be included. The issue with ORF8 is also of importance for regulation of MHC-I, since in a previous study ORF8 was shown to block MHC-I by lysosomal degradation (Zhang et al., PNAS 2021).

We have added to the methods ‘This strain has a genome that is identical to the original Wuhan isolate.’ ORF8 is mutated in Α, but not Wave1 viruses such as England 2. We do see a very minor downregulation of MHC-I, but the magnitude is so low that it is unlikely to be biologically significant. Whether this represents cell-type or HLA-type specific effects of ORF8 is currently unclear, therefore we state in the text ‘A previous study has demonstrated that SARS-CoV2 downregulates HLA-A2 via ORF8, which contrasts somewhat with our results63. This may indicate a HLA- or cell-type specific effect’. However, a full exploration of this phenomenon is tangential to the main results of our study, and beyond the scope of our current manuscript. Although ORF8 antibodies may be useful diagnostically, we do not have any evidence that ORF8 is found on the cell surface – it was not detected in our PMP, and does not have a signal peptide or transmembrane domain. Nor does the paper by Hachim et al. show surface expression. Therefore, it does not currently meet the criteria to be considered as a ADNKA target.

Figure 5C. The finding that the N protein can be detected on the surface of SARS-CoV-2 infected cells is surprising. Has this been reported of before for SARS-CoV-2 or other corona viruses? Is N surface expression found on all infected cells or a specific population of cells (e.g. dying cells)? If N is distributed to the surface only in dying cells it questions the importance of this antigen in triggering ADCC.

Our studies exclude dead cells, therefore this staining is found on live, productively infected cells. We agree that it is surprising, and a major result of our work. This has not been reported for other coronaviruses, but this may simply be because everyone has assumed that it is intracellular, rather than testing it empirically. We have now performed immunofluorescence to provide further evidence for the presence of N on the cell surface (Figure 6D). We also note that nucleoprotein of Flu is also found on the cell surface, and is a target for ADCC. Thus, although this phenomenon is novel for coronaviruses, it is not unique to viruses in general, and we make this point in the discussion.

Figure 6. The authors show that recombinant anti-Spike antibodies bind to SARS-CoV-2 infected cells as determined by flow cytometry, but do not induce ADNKA. This could be due to structural hindrance. Is there an association between antibody epitope recognition and induction of ADNKA? Furthermore, is it possible to further induce CD16 activation by combining anti-S antibodies with varying specificities in one sample to mimic the situation in serum?

It is noticeable that all those that induced ADNKA were targeted at the NTD, and we make this point in the discussion. We have also performed an experiment in which we combined the mAbs. ADNKA still fell far short of the levels of activation seen with serum.

Figure 7. In this figure it is elegantly demonstrated that non-spike SARS-CoV-2 specific antibodies prime cells for ADNKA. Since new SARS-CoV-2 variants escape neutralization by mutation of the Spike protein, it would be relevant to know whether the ability to induce ADNKA still remains equally effective by sera from individuals infected with previous variants

This is an extremely interesting point, which we are currently following up. However, it is a massive program of work, which is far beyond the scope of the current paper, which is already extensive. Our initial data using VOCs indicates that we will need to use reverse genetics systems to make hybrid viruses containing known distinct epitopes from different variants in order to dissect the specific impact of antigenic changes in different proteins of the different VOCs. We will also need to source serums from a large number of people infected with known variants, which has become more complicated as successive waves of different variants have passed through the population. It is therefore impractical to add this level of experimentation to the current manuscript, and is not required in order for us to draw the conclusions that we make.

This study demonstrates the ability of non-Spike SARS-CoV-2 antibodies to induce ADCC. It is indeed surprising that depletion of anti-Spike antibodies has only a modest effect on ADCC. To underline the reduced role of Spike in ADNKA, controls suggested for Figure 6 and 7 should be included. Furthermore, if this is the first report on N protein expression on the cell surface, this point should be strengthened, e.g. by immunofluorescence microscopy.

We are unclear which controls the reviewer is suggesting for Figure 6 and 7, however these sections have now been extended to demonstrate that a Nucleocapsid monoclonal can mediate ADNKA, that depletion of Nucleocapsid antibodies impacts ADNKA, that deletion of ORF3a impacts ADNKA, and that combining Spike MAbs is insufficient to recapitulate the effects of serum. As above, we have now also performed immunofluorescence to demonstrate the presence of Nucleocapsid on the cell surface.

Premature data in Figure 2-3 appear rather distracting here. In my opinion the manuscript would gain in clarity if the ADNKA part would be handled on its own.

We respectfully disagree, we would argue that it is not possible to fully understand the ability of NK cells to control SARS-CoV2 without understanding both the antigens that prime activation, and the ability of the virus to antagonise that activation through manipulation of activatory and inhibitory NK ligands on the cell surface. The modulation of innate ligands described in the early part of the paper may well be the reason that Spike monoclonals are significantly less effective against infected, as opposed to transfected, cells.

[Editors' note: further revisions were suggested prior to acceptance, as described below.]

The manuscript has been improved but there are some remaining issues that need to be addressed, as outlined below:

Reviewer #3:

The revised version of the manuscript by Fielding et al. has been much improved. It now contains more detailed data on the regulation of NKG2D and Nkp30 ligands. The authors investigated the level of regulation and were able to exclude the degradation and retention of these proteins. Instead, Fielding et al., describe a selective regulation by Nsp14 and also Nsp1. This clearly strengthens the manuscript.

In general, the points raised previously have been approached and discussed appropriately.

I recommend publication of the manuscript after the following changes to the manuscript have been made.

One important control is still missing in Figure 6C: a control with uninfected cells should be included.

This control was included, however it was not explicitly described in the legend. This has now been corrected.

https://doi.org/10.7554/eLife.74489.sa2

Article and author information

Author details

  1. Ceri Alan Fielding

    Division of Infection and Immunity, School of Medicine, Cardiff University, Cardiff, United Kingdom
    Contribution
    Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Supervision, Validation, Visualization, Writing - original draft, Writing - review and editing
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-5817-3153

  2. Pragati Sabberwal

    Division of Infection and Immunity, School of Medicine, Cardiff University, Cardiff, United Kingdom
    Contribution
    Formal analysis, Investigation, Methodology
    Competing interests
    No competing interests declared

  3. James C Williamson

    Cambridge Institute for Therapeutic Immunology & Infectious Disease, Jeffrey Cheah Biomedical Centre, Cambridge Biomedical Campus, University of Cambridge, Cambridge, United Kingdom
    Contribution
    Formal analysis, Investigation, Methodology, Writing - review and editing
    Competing interests
    No competing interests declared

  4. Edward JD Greenwood

    Cambridge Institute for Therapeutic Immunology & Infectious Disease, Jeffrey Cheah Biomedical Centre, Cambridge Biomedical Campus, University of Cambridge, Cambridge, United Kingdom
    Contribution
    Formal analysis, Investigation, Methodology
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-5224-0263

  5. Thomas WM Crozier

    Cambridge Institute for Therapeutic Immunology & Infectious Disease, Jeffrey Cheah Biomedical Centre, Cambridge Biomedical Campus, University of Cambridge, Cambridge, United Kingdom
    Contribution
    Formal analysis, Investigation, Methodology
    Competing interests
    No competing interests declared

  6. Wioleta Zelek

    Division of Infection and Immunity, School of Medicine, Cardiff University, Cardiff, United Kingdom
    Contribution
    Formal analysis, Investigation, Methodology
    Competing interests
    No competing interests declared

  7. Jeffrey Seow

    Department of Infectious Diseases, School of Immunology & Microbial Sciences, King's College London, London, United Kingdom
    Contribution
    Formal analysis, Investigation, Methodology, Resources
    Competing interests
    No competing interests declared

  8. Carl Graham

    Department of Infectious Diseases, School of Immunology & Microbial Sciences, King's College London, London, United Kingdom
    Contribution
    Methodology, Resources
    Competing interests
    No competing interests declared

  9. Isabella Huettner

    Department of Infectious Diseases, School of Immunology & Microbial Sciences, King's College London, London, United Kingdom
    Contribution
    Methodology, Resources
    Competing interests
    No competing interests declared

  10. Jonathan D Edgeworth

    1. Department of Infectious Diseases, School of Immunology & Microbial Sciences, King's College London, London, United Kingdom
    2. Department of Infectious Diseases, Guy’s and St Thomas’ NHS Foundation Trust, London, United Kingdom
    Contribution
    Methodology, Resources, Writing - review and editing
    Competing interests
    No competing interests declared

  11. David A Price

    Division of Infection and Immunity, School of Medicine, Cardiff University, Cardiff, United Kingdom
    Contribution
    Funding acquisition, Methodology, Resources, Writing - review and editing
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0001-9416-2737

  12. Paul B Morgan

    Division of Infection and Immunity, School of Medicine, Cardiff University, Cardiff, United Kingdom
    Contribution
    Funding acquisition, Resources, Writing - review and editing
    Competing interests
    No competing interests declared

  13. Kristin Ladell

    Division of Infection and Immunity, School of Medicine, Cardiff University, Cardiff, United Kingdom
    Contribution
    Funding acquisition, Investigation, Methodology, Resources
    Competing interests
    No competing interests declared

  14. Matthias Eberl

    Division of Infection and Immunity, School of Medicine, Cardiff University, Cardiff, United Kingdom
    Contribution
    Funding acquisition, Investigation, Methodology, Project administration, Resources
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-9390-5348

  15. Ian R Humphreys

    Division of Infection and Immunity, School of Medicine, Cardiff University, Cardiff, United Kingdom
    Contribution
    Funding acquisition, Methodology, Project administration, Resources
    Competing interests
    No competing interests declared

  16. Blair Merrick

    1. Department of Infectious Diseases, School of Immunology & Microbial Sciences, King's College London, London, United Kingdom
    2. Department of Infectious Diseases, Guy’s and St Thomas’ NHS Foundation Trust, London, United Kingdom
    Contribution
    Funding acquisition, Methodology, Resources, Writing - review and editing
    Competing interests
    No competing interests declared

  17. Katie Doores

    Department of Infectious Diseases, School of Immunology & Microbial Sciences, King's College London, London, United Kingdom
    Contribution
    Methodology, Resources, Writing - review and editing
    Competing interests
    No competing interests declared

  18. Sam J Wilson

    MRC - University of Glasgow Centre for Virus Research, Glasgow, United Kingdom
    Contribution
    Funding acquisition, Methodology, Resources, Writing - review and editing
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-6065-0895

  19. Paul J Lehner

    Cambridge Institute for Therapeutic Immunology & Infectious Disease, Jeffrey Cheah Biomedical Centre, Cambridge Biomedical Campus, University of Cambridge, Cambridge, United Kingdom
    Contribution
    Funding acquisition, Methodology, Resources, Supervision, Writing - review and editing
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0001-9383-1054

  20. Eddie CY Wang

    Division of Infection and Immunity, School of Medicine, Cardiff University, Cardiff, United Kingdom
    Contribution
    Conceptualization, Funding acquisition, Investigation, Methodology, Project administration, Resources, Supervision, Writing - review and editing
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-2243-4964

  21. Richard J Stanton

    Division of Infection and Immunity, School of Medicine, Cardiff University, Cardiff, United Kingdom
    Contribution
    Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Supervision, Validation, Visualization, Writing - original draft, Writing - review and editing
    For correspondence
    stantonrj@cardiff.ac.uk
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-6799-1182

Funding

National Institute for Health Research (MR/V028448/1)

  • Ceri Alan Fielding
  • Pragati Sabberwal
  • Eddie CY Wang
  • Richard J Stanton

Medical Research Council (MR/S00971X/1)

  • Richard J Stanton

Wellcome Trust (204870/Z/16/Z)

  • Ceri Alan Fielding
  • Eddie CY Wang
  • Richard J Stanton

Ser Cymru

  • Ceri Alan Fielding
  • Eddie CY Wang
  • Richard J Stanton

Medical Research Council (MR/P001602/1)

  • Eddie CY Wang

Medical Research Council (MR/V000489/1)

  • Richard J Stanton

Wellcome Trust (210688/Z/18/Z)

  • Paul J Lehner

Medical Research Council (MR/V011561/1)

  • Paul J Lehner

Wellcome Trust (208354/Z/17/Z)

  • Katie Doores

Medical Research Council (MR/N013700/1)

  • Carl Graham

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication. For the purpose of Open Access, the authors have applied a CC BY public copyright license to any Author Accepted Manuscript version arising from this submission.

Acknowledgements

This work was partly funded by the MRC/NIHR through the UK Coronavirus Immunology Consortium (CiC; MR/V028448/1). RS was supported by the MRC (MR/S00971X/1), Wellcome Trust (204870/Z/16/Z), and Ser Cymru. ECYW was funded by the MRC (MR/P001602/1, MR/V000489/1). PJL was funded by the Wellcome Trust through a Principal Research Fellowship (210688/Z/18/Z), the MRC (MR/V011561/1), the Addenbrooke’s Charitable Trust and the NIHR Cambridge Biomedical Research Centre. KLD was supported by King’s Together Rapid COVID-19 Call, Huo Family Foundation Award, and a Wellcome Trust Multi-User Equipment Grant 208354/Z/17/Z. CG was supported by the MRC-KCL Doctoral Training Partnership in Biomedical Sciences (MR/N013700/1). BM was supported by an NIHR Academic Clinical Fellowship in Combined Infection Training

Ethics

Human subjects: PBMC were extracted from apheresis cones obtained from the Welsh Blood Service (WBS) via an ad-hoc agreement or from blood samples from healthy volunteers and stored in liquid N2 until use. Use of healthy volunteer PBMC for this project, including those from WBS, was ethically approved by the Cardiff University School of Medicine Research Ethics Committee (SMREC) nos. 20/55 and 20/101. Recruitment of healthy volunteers after vaccination was covered by the Cardiff University School of Medicine Research Ethics Committee under reference no. 18/04.

Senior Editor

  1. Betty Diamond, The Feinstein Institute for Medical Research, United States

Reviewing Editor

  1. Stipan Jonjic, University of Rijeka, Croatia

Reviewer

  1. Domenico Mavilio, Humanitas Clinical and Research Center, Italy

Version history

  1. Preprint posted: April 6, 2021 (view preprint)
  2. Received: October 6, 2021
  3. Accepted: May 17, 2022
  4. Accepted Manuscript published: May 19, 2022 (version 1)
  5. Accepted Manuscript updated: May 20, 2022 (version 2)
  6. Version of Record published: June 28, 2022 (version 3)

Copyright

© 2022, Fielding et al.

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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  1. Ceri Alan Fielding
  2. Pragati Sabberwal
  3. James C Williamson
  4. Edward JD Greenwood
  5. Thomas WM Crozier
  6. Wioleta Zelek
  7. Jeffrey Seow
  8. Carl Graham
  9. Isabella Huettner
  10. Jonathan D Edgeworth
  11. David A Price
  12. Paul B Morgan
  13. Kristin Ladell
  14. Matthias Eberl
  15. Ian R Humphreys
  16. Blair Merrick
  17. Katie Doores
  18. Sam J Wilson
  19. Paul J Lehner
  20. Eddie CY Wang
  21. Richard J Stanton
(2022)
SARS-CoV-2 host-shutoff impacts innate NK cell functions, but antibody-dependent NK activity is strongly activated through non-spike antibodies
eLife 11:e74489.
https://doi.org/10.7554/eLife.74489

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