An automated feeding system to precisely control feeding in the killifish opens new areas to explore vertebrate lifespan, cognitive decline, dietary interventions, and drug screening in a high-throughput manner.
A single-cell transcriptome atlas reveals meniscal microenvironment variations during degeneration and cellular heterogeneity foundations of the inner–outer zone differences, suggesting novel cell subtypes as potential therapeutic targets.
Scratch-AID, a deep learning-based system for automatic quantification of mouse scratching behavior, could replace labor-intensive manual quantification and facilitate high through-put anti-itch drug screening.
Specific to muscles and essential for their development, desmin is revealed to be fundamental to the mechanics and shape maintenance of model muscle tissue even in early differentiating and unorganized systems through an integrated magnetic tensiometer approach.
Christiane Pleuger, Dingding Ai ... Andreas Meinhardt
A strategical positioning of resident immune cells are key in determining a tightly controlled immune environment in the different regions of the mouse epididymis that creates unique spatial milieus for sperm maturation and combatting invading pathogens.
Daan B Boltje, Jacob P Hoogenboom ... Sander den Hoedt
Integrating a cryogenic light microscope with a focused ion beam/scanning electron microscope, allows directly targeting of fluorescent structure when preparing a frozen-hydrated lamella, without the need for repositioning or fiducial markers.
Johannes Elferich, Giulia Schiroli ... Nikolaus Grigorieff
Cryo-electron microscopy can be used to detect biomolecules in entire 100- to 250-nm-thick cell slices when using a method to collect montages that prevents radiation damage in areas that have not been imaged yet.
Feng Xian, Julia Regina Sondermann ... Manuela Schmidt
Proteomics uncovers thus far unknown age and sex differences in sciatic nerve and skin of naïve mice, highlighting the importance of adequate age matching and parallel investigation of male and female mice in biomedical studies.
GLMsingle is a publicly available toolbox for analyzing fMRI time-series data that leverages data-driven optimization techniques to improve the accuracy of single-trial BOLD response estimates.
