Patrick C Hoffmann, Stefano L Giandomenico ... Wanda Kukulski
Combining cerebral organoid technology with cryo-correlative microscopy reveals the organization of cytoskeleton, membrane compartments, and protein synthesis machinery contributing to the rapid expansion of developing human axons.
Fluorescence fluctuation spectroscopy is implemented for detection of up to four molecular species, allowing users to quantify molecular interactions and stoichiometry of multicomponent complexes in live cells, in a wide range of biological processes, from membrane signaling to viral assembly.
A statistically principled approach developed to estimate phase of rhythmic signals in real-time shows robustness to multiple sources of error and also provides confidence criteria.
Stephen R Piccolo, Zachary E Ence ... Andrea H Bild
Descriptions, examples, and tools to enable biologists to use the Common Workflow Language for data processing and to facilitate computational reproducibility.
William N Zagotta, Brandon S Sim ... Sharona E Gordon
Time-resolved transition metal ion fluorescence resonance energy transfer using a fluorescent, noncanonical amino acid donor and metal ion acceptor measures distances and distance distributions in proteins, allowing the equilibrium distributions among conformational states to be accurately determined.