HaloTag becomes resistant to lysosomal proteolysis upon ligand binding, enabling us to measure various types of autophagic flux easily and precisely by using HaloTag-containing reporters for processing assays.
A curated resource to study the brain RNA expression patterns of the genes that regulate palmitoylation is presented, alongside a detailed exploration of how these patterns can inform future research.
A novel approach together with a user-friendly web-based analytic tool, coined 'REAP', was proposed to improve the quantitative assessment of dose-response relationship and drug potency.
Elena Bollati, Niclas H Lyndby ... Daniel Wangpraseurt
Scalar irradiance microsensor measurements performed inside the tissue of living corals show that absorption and fluorescence emission by host pigments produce dramatic spectral alterations in the light environment experienced by the symbionts.
Massimo Andreatta, Ariel Tjitropranoto ... Santiago J Carmona
Description of the transcriptional and clonal landscape of virus-specific CD4+ T cells in acute and chronic viral infections, and a new reference map to interpret CD4 T cell diversity across tissues and biological models.
Brooke D Huisman, Zheng Dai ... Michael E Birnbaum
Yeast surface-displayed libraries, when coupled with pooled oligonucleotide synthesis and next-generation sequencing, can be used as a platform to assess binding of whole viral proteomes to class II major histocompatibility complex proteins.
Henriette Hoffmann-Veltung, Nsoh Godwin Anabire ... Maria del Pilar Quintana
An improved 'plug-and-play' reversed FluoroSpot assay is described and validated that allows the simultaneous assessment of antibody cross-reactivity at the single-cell level against up to four variants of a polymorphic antigen of interest.
