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Page 55 of 94
    1. Cell Biology
    2. Genetics and Genomics

    A high-throughput small molecule screen identifies farrerol as a potentiator of CRISPR/Cas9-mediated genome editing

    Weina Zhang, Yu Chen ... Zhiyong Mao
    Farrerol promotes the efficiency of CRISPR/Cas9-mediated genome editing in both cells and embryos.
    1. Immunology and Inflammation
    2. Neuroscience

    A new genetic strategy for targeting microglia in development and disease

    Gabriel L McKinsey, Carlos O Lizama ... Thomas D Arnold
    P2ry12-CreER robustly and specifically labels microglia in fate-mapping and ribosomal profiling experiments, revealing new markers for myeloid subpopulations in the central nervous system.
    1. Immunology and Inflammation

    Studying the biology of cytotoxic T lymphocytes in vivo with a fluorescent granzyme B-mTFP knock-in mouse

    Praneeth Chitirala, Hsin-Fang Chang ... Jens Rettig
    A new knock-in mouse endogenously labels cytotoxic granules and can be used with all modern super-resolution imaging techniques to study T cell function in vitro and in vivo.
    1. Cancer Biology
    2. Cell Biology

    Tracking cells in epithelial acini by light sheet microscopy reveals proximity effects in breast cancer initiation

    Ashna Alladin, Lucas Chaible ... Martin Jechlinger
    The presented primary epithelial acinus-based platform for studying interactions between normal and malignant cells will enable in depth analysis of the tumor initiation process on a molecular level.
    1. Neuroscience

    Sensory restoration by epidural stimulation of the lateral spinal cord in upper-limb amputees

    Santosh Chandrasekaran, Ameya C Nanivadekar ... Lee E Fisher
    Cervical spinal cord stimulation evokes sensory percepts in the missing hand and arm of people with upper-limb amputation, regardless of amputation level or time post-amputation.
    1. Computational and Systems Biology
    2. Neuroscience

    A taxonomy of seizure dynamotypes

    Maria Luisa Saggio, Dakota Crisp ... William C Stacey
    Taxonomy of seizure dynamics (TSD) provides a rigorous method for classifying and quantifying seizures and a principled framework for understanding seizure initiation and propagation.
    1. Immunology and Inflammation
    2. Neuroscience

    Differential accumulation of storage bodies with aging defines discrete subsets of microglia in the healthy brain

    Jeremy Carlos Burns, Bunny Cotleur ... Michael Mingueneau
    Two novel subsets of microglia identified by their unique autofluorescence profiles differ in their subcellular organization, proteomic signatures and in their response to aging and lysosomal dysfunction.
    1. Cell Biology

    Assigning mitochondrial localization of dual localized proteins using a yeast Bi-Genomic Mitochondrial-Split-GFP

    Gaétan Bader, Ludovic Enkler ... Hubert Dominique Becker
    The Bi-Genomic Mitochondrial-Split-GFP, where both fragments of the Split-GFP are expressed by separated translation machineries, shuts off cytosolic fluorescence of dual-localized proteins, allowing visualization of their mitochondrial echoforms.
    1. Microbiology and Infectious Disease

    Droplet-based high-throughput cultivation for accurate screening of antibiotic resistant gut microbes

    William J Watterson, Melikhan Tanyeri ... Savaş Tay
    High-throughput droplet-based cultivation of gut microbes reduces biases of traditional cultivation strategies and thereby enables detection of difficult-to-culture organisms, which is required in applications such as antibiotic screening.
    1. Neuroscience

    A Matlab-based toolbox for characterizing behavior of rodents engaged in string-pulling

    Samsoon Inayat, Surjeet Singh ... Majid H Mohajerani
    Analysis methods for video data of rodents engaged in string-pulling behavior have been developed and used to highlight differences in motion profiles of Swiss Webster and C57Bl/6 mice.