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    1. Biochemistry and Chemical Biology
    2. Cell Biology

    Systematic characterisation of site-specific proline hydroxylation using hydrophilic interaction chromatography and mass spectrometry

    Hao Jiang, Jimena Druker ... Angus I Lamond
    A robust workflow for the identification of proline hydroxylation sites in proteins was established, using hydrophilic interaction chromatography enrichment and LC-MS/MS together with refining and filtering parameters during data analysis.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Structural insights into the recruitment of viral type 2 IRES to ribosomal preinitiation complex for protein synthesis

    Deepakash Das, Tanweer Hussain
    The viral type 2 internal ribosome entry site uses a unique mechanism to hijack the host ribosomal machinery by interacting with initiator tRNA and 40S head.
    1. Physics of Living Systems

    Controlling the synchronization and symmetry breaking of coupled bacterial pili on active biofilm carpets

    Baha Altın, Enes Talha Günay ... Askin Kocabas
    Active biofilm carpets generate Mexican-wave-like synchronized dynamics and topological defects through nonreciprocal pili interactions.
    1. Microbiology and Infectious Disease

    Mycobacterium tuberculosis partitions the Krebs cycle under iron starvation

    Agnese Serafini, Acely Garza-Garcia ... Riccardo Manganelli
    Mycobacterium tuberculosis rewires central carbon metabolism to circumvent iron-dependent metabolic routes during iron starvation.
    1. Neuroscience

    Hippocampal neuronal and astrocytic responses to noradrenaline and natural arousal

    Sian N Duss, Maria Wilhelm ... Peter Rupprecht
    Not revised
    Reviewed Preprint v1
    • Important
    • Solid
    1. Biochemistry and Chemical Biology
    2. Microbiology and Infectious Disease

    Acyl Carrier Protein is Essential for Apicoplast Biogenesis in Malaria Parasites Independent of Fatty Acid Synthesis

    Sage WR Geher, Seyi Falekun ... Paul A Sigala
    Not revised
    Reviewed Preprint v1
    • Important
    • Incomplete
    1. Neuroscience

    Feedback control of recurrent circuits imposes dynamical constraints on learning

    Harsha Gurnani, Weixuan Liu, Bingni W Brunton
    Not revised
    Reviewed Preprint v1
    • Important
    • Convincing
    1. Neuroscience

    An applicable and efficient retrograde monosynaptic circuit mapping tool for larval zebrafish

    Tian-Lun Chen, Qiu-Sui Deng ... Xu-Fei Du
    Revised
    Reviewed Preprint v3
    Updated
    • Important
    • Compelling
    1. Genetics and Genomics

    A pilot study for whole proteome tagging in Caenorhabditis elegans

    Matthew Eroglu, Oliver Hobert
    Simultaneous tagging of multiple genes with different fluorophores provides a proof of concept for the scalability of tagging all genes in the Caenorhabditis elegans genome.
    1. Neuroscience

    Arousal modulates functional connectivity through structured and hemispherically asymmetric community architecture during wakefulness

    Xiangyu Kong, Siyu Li, Gaolang Gong
    Rather than exerting a uniform influence, arousal modulates the connectome through a structured, low-dimensional community architecture characterized by discrete topological patterns and intrinsic hemispheric asymmetries.
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