Soon after fertilisation, a critical portion of the embryonic genome is switched on through the actions of maternally inherited Stella, in part through controlling the activation of transposable elements.
Matthew T Parker, Katarzyna Knop ... Gordon G Simpson
Sequencing mRNA directly with nanopores can reveal the authentic combinations of multiple mRNA processing events in full-length mRNA molecules in addition to identifying base modifications.
A new approach combines guide RNA multiplexing with CRISPR activation and interference to facilitate functional studies of transposable elements present in hundreds of copies throughout the human genome.
Dayana C Farhat, Matthew W Bowler ... Christopher Swale
The epitranscriptomic-driven mRNA polyadenylation pathway protects transcriptome integrity by restricting transcriptional read-throughs and RNA chimera formation in apicomplexan parasites and plants.
Matthew T Parker, Katarzyna Knop ... Gordon G Simpson
The expression of Arabidopsis NLR immune response genes is modulated by premature transcription termination, and this has implications for understanding NLR regulation and evolutionary dynamics.
De novo transcriptome assembly and comprehensive characterization of gene expression in proliferating cells of regeneration-capable flatworm Macrostomum lignano advance this organism as a powerful model for stem cell research.
The first genomic view of beetle luciferase evolution indicates evolutionary independence of luciferase between fireflies and click-beetles, and provide valuable datasets which will accelerate the discovery of new biotechnological tools.