The crystal structures of the intracellular part of the plexin receptor in the active dimer form, and its complex with a key downstream signalling protein Rap, provide insights into how plexin initiates a signalling cascade involved in axon guidance.
Jeremiah Keyes, Ambhighainath Ganesan ... Jin Zhang
An individual extracellular signal regulates multiple cellular actions through differences in the temporal dynamics of spatially distinct populations of the central signaling enzyme, extracellular-signal regulated kinase.
Hemoglobin affinity and cooperativity reveal mechanistic insights in how the relation between physiology and evolutionary variations shape a protein's molecular property.
Plexin controls the spatial distribution of synapses by locally inhibiting Rap2 small GTPase activity along the axon, and a Rap2 effector, TNIK, which also plays a key role in inhibiting synapse number.
Kathleen Greenham, Carmela Rosaria Guadagno ... C Robertson McClung
Coupled physiology and gene expression, measured over a two-day time course, reveals specific time-of-day responses to the early stages of drought in Brassica rapa.
Christian Keine, Mohammed Al-Yaari ... Samuel M Young Jr
A combination of genetic, electrophysiological, and modeling approaches reveals that presynaptic Rac1 is a key molecule that controls synaptic strength and plasticity by regulating the synaptic vesicle cycle steps controlling the number of fusion competent synaptic vesicles.
Kathleen Greenham, Ryan C Sartor ... C Robertson McClung
Whole genome duplication in Brassica rapa is accompanied by both expansion of the circadian transcriptome and widespread temporal reconfiguration of gene regulatory networks consistent with subfunctionalization among pairs of paralogs.
Inactivation of KAP1 by mTOR-mediated phosphorylation releases human cytomegalovirus from latency, and has the potential to be used as a therapy to purge the virus from transplant organs.
Brian A Sosa, F Esra Demircioglu ... Thomas U Schwartz
LAP1 adopts an AAA+ like fold that, while unable to bind nucleotide, can enhance ATPase activity in the neighboring TorsinA protomer in an unusual heterohexameric ring, via an arginine finger.
Simultaneous phosphorylation of multiple substrate motifs drives switch-like target recognition and monoubiquitylation by an E3 ligase during metazoan development.
