4,893 results found
    1. Structural Biology and Molecular Biophysics
    2. Computational and Systems Biology

    Pi-Pi contacts are an overlooked protein feature relevant to phase separation

    Robert McCoy Vernon, Paul Andrew Chong ... Julie Deborah Forman-Kay
    Statistics on the frequencies of pi interactions in folded protein structures enable successful prediction of intrinsically disordered protein phase separation, with clear implications for a physical understanding of cellular organization.
    1. Biochemistry and Chemical Biology
    2. Microbiology and Infectious Disease

    Dissecting the phase separation and oligomerization activities of the carboxysome positioning protein McdB

    Joseph L Basalla, Claudia A Mak ... Anthony G Vecchiarelli
    Carbon-fixing organelles, called carboxysomes, link to their spatial organization system in the bacterial cell by a hexameric protein that forms pH-dependent condensates via a nuanced multidomain mechanism.
    1. Computational and Systems Biology
    2. Neuroscience

    Narrow equilibrium window for complex coacervation of tau and RNA under cellular conditions

    Yanxian Lin, James McCarty ... Songi Han
    Liquid-liquid phase separation of tau is demonstrated to be an equilibrium state, stable only within a narrow range near physiological conditions, and thus has the capacity to regulate biological processes.
    1. Cell Biology

    Multiple polarity kinases inhibit phase separation of F-BAR protein Cdc15 and antagonize cytokinetic ring assembly in fission yeast

    Rahul Bhattacharjee, Aaron R Hall ... Kathleen L Gould
    Biochemical studies and molecular dynamics simulations indicate that dephosphroylation of F-BAR protein Cdc15 promotes cytokinesis ring assembly in yeast via conformation change of intrinsically disorder regions and condensate formation.
    1. Physics of Living Systems

    A scale-invariant log-normal droplet size distribution below the critical concentration for protein phase separation

    Tommaso Amico, Samuel Toluwanimi Dada ... Amos Maritan
    The scaling invariance of the droplet size distribution as a universal aspect of protein phase separation is reported, providing a quantitative approach to determinate the critical concentration for this process.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Phosphorylation, disorder, and phase separation govern the behavior of Frequency in the fungal circadian clock

    Daniyal Tariq, Nicole Maurici ... Brian R Crane
    The intrinsic disorder of the circadian clock protein Frequency organizes binding partners, facilitates liquid–liquid phase separation, modulates Frequency phosphorylation, and derives from sequence properties conserved with homologous clock components.
    1. Cell Biology
    2. Developmental Biology

    Spatial patterning of P granules by RNA-induced phase separation of the intrinsically-disordered protein MEG-3

    Jarrett Smith, Deepika Calidas ... Geraldine Seydoux
    A competition for RNA drives the localized assembly of RNA granules in the C. elegans embryo.
    1. Structural Biology and Molecular Biophysics

    Peptides that Mimic RS repeats modulate phase separation of SRSF1, revealing a reliance on combined stacking and electrostatic interactions

    Talia Fargason, Naiduwadura Ivon Upekala De Silva ... Jun Zhang
    Short peptides that mimic the regions responsible for phase separation can be used to solubilize phase-separating proteins in their native states and to determine the mechanism of phase separation.
    1. Physics of Living Systems

    Spatial control of irreversible protein aggregation

    Christoph Weber, Thomas Michaels, L Mahadevan
    A theoretical basis for the spatial regulation of protein aggregation shows how aggregates can partition preferentially into intracellular liquid compartments.
    1. Cell Biology
    2. Physics of Living Systems

    Fixation can change the appearance of phase separation in living cells

    Shawn Irgen-Gioro, Shawn Yoshida ... Shasha Chong
    Different fixation protocols can both enhance and diminish the appearance of liquid–liquid phase separation (LLPS) droplets presenting a caveat in using fixed-cell imaging to diagnose LLPS.

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