Fast fluctuations of oxygen, linked to behavioral and neural dynamics in vivo, cause phasic transients is amperometry-measured cholinergic signals via the mechanism of non-steady-state enzyme kinetics in the choline-oxidase-based biosensor.
Structural, biochemical, and cell biology study revealed that Rsu1, through binding to PINCH1, inhibits the actin bundling ability of ILK/PINCH/Parvin to regulate the actin dynamics at focal adhesion.
The structure-based design established a new approach to control pathway-selective activation of opioid receptors, resulting in new dual MOR/KOR G-protein biased agonist analgesics with attenuated liabilities.
Rachel Knoener, Edward Evans III ... Lloyd M Smith
The distinct protein-RNA interactomes of HIV-1 RNA splice forms are revealed using a powerful multiplex strategy for RNA capture and mass spectrometric analysis.
Simultaneous cytokine signaling results in unexpected transcription factor changes that fuel a cellular response divergent from the sum of each cytokine alone.
Aaron Snoberger, Bipasha Barua ... E Michael Ostap
Mechanochemical defects of a β-cardiac myosin mutation that results in severe hypertrophic cardiomyopathy are reported and the heart failure drug omecamtiv mecarbil rescues these defects.
The cotranslational membrane integration of three multispanning Escherichia coli inner membrane proteins is followed using force profile analysis, uncovering unexpected complexities in the membrane integration process.
