During CRISPR adaptation, Cas4 forms a ternary complex with the Cas1-Cas2 spacer integration complex, an interaction that coordinates substrate hand-off following precise, PAM-dependent prespacer processing prior to integration.
The nutrients available in some tumors and the factors that influence tumor nutrient availability are characterized, which provides insight into the metabolic constraints of the tumor microenvironment.
Both exogenous and endogenous genes can be highly activated in cells by a new dCas9-based activator, CRISPR-assisted trans enhancer, that can be used to activate genes for biomedical applications.
Ruben M Atilho, Gayan Mirihana Arachchilage ... Ronald R Breaker
Many bacteria use a tiny riboswitch aptamer to sense the thiamin precursor HMP-PP to regulate the production of another thiamin precursor HET-P to efficiently biosynthesize this essential coenzyme.
Single molecule DNA-binding trajectories and deterministic modeling analyses demonstrate a functional role for high energy partly folded states in Transcription Activator-Like Effectors that could improve future TALEN design.
Zachary F Hallberg, Chi Ho Chan ... Ming C Hammond
A newfound signaling enzyme that diverged from a protein family ubiquitous in bacteria provides mechanistic insights into how new signaling activity emerges to control distinct cellular function and physiology.
Blake W Tye, Nicoletta Commins ... L Stirling Churchman
Rapidly proliferating cells are at risk of compromised cell fitness due to proteostasis collapse from perturbations that interfere with ribosome biogenesis.
Marijn de Boer, Giorgos Gouridis ... Thorben Cordes
Selective import via bacterial ABC importers is facilitated by a hitherto unrecognized complexity in the conformational dynamics of the substrate-binding proteins.
