Stress-regulated secretion of an intestinal peptide positively regulates the antioxidant response by promoting neuropeptide release from the nervous system, defining a gut-to-brain-to-gut endocrine axis in the oxidative stress response.
Anne Drougard, Eric H Ma ... John Andrew Pospisilik
High-fat ingestion triggers microglial metabolic activation within a single feeding cycle, and enhances whole-body metabolism and memory by converting fatty acids into protective substrates for surrounding neurons.
Aging reduces the potassium M current in sympathetic motor neurons, resulting in a hyperactive phenotype that can be reversed by pharmacological activation of these channels.
John H Day, Catherine M Della Santina ... Laurie A Boyer
A simple and accessible method for high-throughput super-resolution fluorescence microscopy that is compatible with commonly used staining practices, 96-well cell culture plates, and confocal microscopes.
Gradual downregulation of each of the subunits of the evolutionary-conserved exocyst holocomplex shows that the complex is required for biogenesis, maturation, and exocytosis of secretory granules in Drosophila salivary glands.
The interaction between the sperm hook and the female reproductive tract, including various sperm dynamics related to their movement through the reproductive tract, has been directly observed for the first time using deep tissue live imaging.
Fatima Tleiss, Martina Montanari ... C Leopold Kurz
Bacterial location quantifications highlights how Drosophila melanogaster larvae discriminate bacteria to isolate and later eliminate pathogens in the anterior midgut through coordinated mechanisms involving reactive oxygen species (ROS) and antimicrobial peptides (AMPs).
The USP-50/USP8 protease removes the Rab5 GEF Rabex5 from endosomes while facilitating recruitment of the Rab7 GEF SAND-1/Mon1, promoting endosome maturation.
The automated workflow significantly enhances the precision and scalability of cell cycle analysis, enabling high-throughput studies in both non-adherent and adherent cells for advanced cancer research and drug development.
