Two alternative models of PKA activation were tested in neurons, and the results support the classical model in which the catalytic subunit dissociated from the regulatory subunit upon activation.
Chromatin immunoprecipitation sequencing and RNA sequencing have elucidated the complex transcript regulation pathways orchestrated by SntB, pivotal for Aspergillus flavus's development, mycotoxin production, and virulence.
SIRT4 regulates cellular communication network factor 2 expression through U2 small nuclear RNA auxiliary factor 2-mediated pre-mRNA splicing, presenting a potential therapeutic target for kidney fibrosis.
Improved technology for counting proteins captures myosin 10 dynamics throughout the filopodial lifecycle, defines requirements for initiating filopodia, and reveals a crowded filopodial tip where motors frequently exceed available actin.
The established triple co-culture blood-–brain barrier (BBB) model could be used for predicting drugs’ BBB permeability, and co-culture with neurons and astrocytes enhanced brain endothelial cells’ integrity by secreting GDNF, which upregulated claudin-5 and VE-cadherin expression.
