Johanna Odenwald, Bernardo Gabiatti ... Susanne Kramer
Proteins that are low abundant and/or poorly accessible to antibodies can be readily localised with fluorescent streptavidin, when expressed fused to a biotin ligase.
BoNT intoxication of neurons is revisited using a genome-wide siRNA screen and organelle-specific reporters, suggesting a mechanism of translocation to the cytosol fundamentally different than previously proposed.
The formation of large endolysosomal structures in unfertilized eggs ensures that lysosomes remain dormant before fertilization, and then shift into clean-up mode after the egg-to-embryo transition.
